PMID- 31806438 OWN - NLM STAT- MEDLINE DCOM- 20201230 LR - 20221207 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 388 DP - 2020 Apr 15 TI - High-rate anaerobic decolorization of methyl orange from synthetic azo dye wastewater in a methane-based hollow fiber membrane bioreactor. PG - 121753 LID - S0304-3894(19)31707-8 [pii] LID - 10.1016/j.jhazmat.2019.121753 [doi] AB - Anaerobic biological techniques are widely used in the reductive decolorization of textile wastewater. However, the decolorization efficiency of textile wastewater by conventional anaerobic biological techniques is generally limited due to the low biomass retention capacity and short hydraulic retention time (HRT). In this study, a methane-based hollow fiber membrane bioreactor (HfMBR) was initially inoculated with an enriched anaerobic methane oxidation (AOM) culture to rapidly form an anaerobic biofilm. Then, synthetic azo dye wastewater containing methyl orange (MO) was fed into the HfMBR. MO decolorization efficiency of approximately 100 % (HRT = 2 to 1.5 days) and maximum decolorization rate of 883 mg/L/day (HRT = 0.5 day) were obtained by the stepwise increase of the MO loading rate into the methane-based HfMBR. Scanning electron microscopy (SEM) and fluorescence in situ hybridization (FISH) analysis visually revealed that archaea clusters formed synergistic consortia with adjacent bacteria. Quantitative PCR (qPCR), phylogenetic and high-throughput sequencing analysis results further confirmed the biological consortia formation of methane-related archaea and partner bacteria, which played a synergistic role in MO decolorization. The high removal efficiency and stable microbial structure in HfMBR suggest it is a potentially effective technique for high-toxic azo dyes removal from textile wastewater. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Bai, Ya-Nan AU - Bai YN AD - Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou, PR China. FAU - Wang, Xiu-Ning AU - Wang XN AD - CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. FAU - Zhang, Fang AU - Zhang F AD - Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China. Electronic address: zhfang@fafu.edu.cn. FAU - Wu, Jun AU - Wu J AD - CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. FAU - Zhang, Wei AU - Zhang W AD - Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. FAU - Lu, Yong-Ze AU - Lu YZ AD - CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. FAU - Fu, Liang AU - Fu L AD - CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. FAU - Lau, Tai-Chu AU - Lau TC AD - Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou, PR China; State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hong Kong. FAU - Zeng, Raymond J AU - Zeng RJ AD - Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, PR China; Advanced Laboratory for Environmental Research and Technology, USTC-CityU, Suzhou, PR China; CAS Key Laboratory of Urban Pollutant Conversion, Department of Applied Chemistry, University of Science and Technology of China, Hefei 230026, PR China. Electronic address: rzeng@ustc.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191125 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 RN - 0 (Azo Compounds) RN - 0 (Membranes, Artificial) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Waste Water) RN - 0 (Water Pollutants, Chemical) RN - 6B4TC34456 (methyl orange) RN - OP0UW79H66 (Methane) SB - IM MH - Anaerobiosis MH - Azo Compounds/*analysis MH - Biofilms/growth & development MH - Bioreactors/*microbiology MH - *Membranes, Artificial MH - Methane/*metabolism MH - Methanosarcinaceae/genetics/growth & development MH - Phylogeny MH - Proteobacteria/genetics/growth & development MH - RNA, Ribosomal, 16S MH - Wastewater/*chemistry MH - Water Decolorization/*methods MH - Water Pollutants, Chemical/*analysis OTO - NOTNLM OT - Anaerobic methane oxidation OT - Archaea and bacteria OT - Decolorization OT - Hollow fiber membrane bioreactor OT - Methyl orange COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2019/12/07 06:00 MHDA- 2020/12/31 06:00 CRDT- 2019/12/07 06:00 PHST- 2019/10/22 00:00 [received] PHST- 2019/11/23 00:00 [revised] PHST- 2019/11/23 00:00 [accepted] PHST- 2019/12/07 06:00 [pubmed] PHST- 2020/12/31 06:00 [medline] PHST- 2019/12/07 06:00 [entrez] AID - S0304-3894(19)31707-8 [pii] AID - 10.1016/j.jhazmat.2019.121753 [doi] PST - ppublish SO - J Hazard Mater. 2020 Apr 15;388:121753. doi: 10.1016/j.jhazmat.2019.121753. Epub 2019 Nov 25.