PMID- 31806443
OWN - NLM
STAT- MEDLINE
DCOM- 20201230
LR  - 20201230
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 388
DP  - 2020 Apr 15
TI  - Identification of adverse outcome pathway related to high-density polyethylene 
      microplastics exposure: Caenorhabditis elegans transcription factor RNAi 
      screening and zebrafish study.
PG  - 121725
LID - S0304-3894(19)31679-6 [pii]
LID - 10.1016/j.jhazmat.2019.121725 [doi]
AB  - To gain insight into the human health implications of microplastics, in this 
      study, we investigated the possible mechanisms affecting the toxicity of 
      high-density polyethylene (HDPE) in the nematode Caenorhabditis elegans using 
      RNAi screening and a bioinformatics-based unbiased approach. The candidate 
      pathways identified from C. elegans study were also confirmed using vertebrate 
      model, zebrafish, Danio rerio and human relevance was then inferred using 
      Comparative Toxicogenomics Database (CTD) analysis. Prior to evaluating the 
      toxicity, label-free Raman mapping was conducted to investigate whether or not 
      the organisms could uptake HDPE. C. elegans transcription factor RNAi screening 
      results showed that the nucleotide excision repair (NER) and transforming growth 
      factor-beta (TGF-beta) signaling pathways were significantly associated with HDPE 
      exposure, which was also confirmed in zebrafish model. Gene-disease interaction 
      analysis using the CTD revealed the possible human health implications of 
      microplastics. Finally, based on this finding, related AOPs were identified from 
      AOP Wiki (http://aopwiki.org), which are "Peroxisome proliferator-activated 
      receptors gamma inactivation leading to lung fibrosis" and "AFB1: Mutagenic 
      Mode-of-Action leading to Hepatocellular Carcinoma". Further studies are needed 
      for the validation of these AOPs with various microplastics.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Kim, Youngho
AU  - Kim Y
AD  - School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, 
      Dongdaemun-gu, Seoul, 02504, Republic of Korea.
FAU - Jeong, Jaeseong
AU  - Jeong J
AD  - School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, 
      Dongdaemun-gu, Seoul, 02504, Republic of Korea.
FAU - Lee, Seungki
AU  - Lee S
AD  - Department of Life Science, University of Seoul, 163 Seoulsiripdae-ro, 
      Dongdaemun-gu, Seoul, 02504, Republic of Korea.
FAU - Choi, Inhee
AU  - Choi I
AD  - Department of Life Science, University of Seoul, 163 Seoulsiripdae-ro, 
      Dongdaemun-gu, Seoul, 02504, Republic of Korea.
FAU - Choi, Jinhee
AU  - Choi J
AD  - School of Environmental Engineering, University of Seoul, 163 Seoulsiripdae-ro, 
      Dongdaemun-gu, Seoul, 02504, Republic of Korea. Electronic address: 
      jinhchoi@uos.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191120
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Microplastics)
RN  - 0 (Transcription Factors)
RN  - 0 (Zebrafish Proteins)
SB  - IM
MH  - Adverse Outcome Pathways
MH  - Animals
MH  - Caenorhabditis elegans/*drug effects/genetics
MH  - Caenorhabditis elegans Proteins/genetics
MH  - Embryo, Nonmammalian/drug effects
MH  - Environmental Pollutants/*toxicity
MH  - Gene Expression Profiling
MH  - Humans
MH  - Microplastics/*toxicity
MH  - RNA Interference/*drug effects
MH  - Transcription Factors/*genetics
MH  - Transcriptome
MH  - Zebrafish/*genetics
MH  - Zebrafish Proteins/genetics
OTO - NOTNLM
OT  - Adverse outcome pathway (AOP)
OT  - Caenorhabditis elegans
OT  - High-density polyethylene
OT  - Nucleotide excision repair (NER)
OT  - Transforming growth factor-beta (TGF-beta)
COIS- Declaration of Competing Interest None.
EDAT- 2019/12/07 06:00
MHDA- 2020/12/31 06:00
CRDT- 2019/12/07 06:00
PHST- 2019/08/31 00:00 [received]
PHST- 2019/11/19 00:00 [revised]
PHST- 2019/11/19 00:00 [accepted]
PHST- 2019/12/07 06:00 [pubmed]
PHST- 2020/12/31 06:00 [medline]
PHST- 2019/12/07 06:00 [entrez]
AID - S0304-3894(19)31679-6 [pii]
AID - 10.1016/j.jhazmat.2019.121725 [doi]
PST - ppublish
SO  - J Hazard Mater. 2020 Apr 15;388:121725. doi: 10.1016/j.jhazmat.2019.121725. Epub 
      2019 Nov 20.