PMID- 31807152
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 6
DP  - 2019 Dec
TI  - Therapeutic effect of methylprednisolone combined with high frequency 
      electrotherapy on acute spinal cord injury in rats.
PG  - 4682-4688
LID - 10.3892/etm.2019.8147 [doi]
AB  - Acute spinal cord injury (SCI) has a high rate of disability and mortality. 
      Although secondary SCI results in local tissue hypoxia and the release of 
      inflammatory mediators, it is both controllable and reversible. Therefore, timely 
      rehabilitation treatment is beneficial for the partial recovery of patients with 
      SCI. The present study aimed to investigate the use of methylprednisolone 
      combined with high-frequency electrotherapy as a method of rehabilitation 
      treatment in rats with SCI. The rat SCI model was prepared using the modified 
      Allen's method with the animals randomly divided into the following 4 groups 
      (n=10 for each group): SCI; methylprednisolone (300 mg/kg); high-frequency 
      electrotherapy; and combination treatment with electrotherapy combined with 
      methylprednisolone (300 mg/kg). The Basso, Beattie and Bresnahan (BBB) score, 
      somatosensory evoked potential (SEP) and motor evoked potential (MEP) were used 
      to assess spinal function. Brain-derived neurotrophic factor (BDNF) and NF-kappaB 
      expression levels were detected using reverse transcription-quantitative PCR and 
      western blotting. Tumor necrosis factor (TNF)-alpha and IL-2 expression levels were 
      determined by ELISA, and caspase 3 activity was also assessed. In all treatment 
      groups, BDNF mRNA and protein expression levels were significantly increased, 
      whilst those of NF-kappaB were reduced. Additionally, an elevated BBB score, improved 
      SEPs and MEPs, inhibited caspase 3 activity and downregulated TNF-alpha and IL-2 
      expression levels were observed, compared with the SCI group (P<0.05). However, 
      the combination group exhibited more significant effects on SCI. In conclusion, 
      methylprednisolone combined with high frequency electrotherapy may improve the 
      symptoms of SCI by increasing the expression level of BDNF, reducing that of 
      NF-kappaB, and suppressing the secretion of inflammatory factors.
CI  - Copyright: (c) Li et al.
FAU - Li, Shuiqin
AU  - Li S
AD  - Department of Rehabilitation Medicine, The First Affiliated Hospital of Xi'an 
      Medical University, Xi'an, Shaanxi 710077, P.R. China.
FAU - Ou, Yan
AU  - Ou Y
AD  - Department of Nephrology, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710004, P.R. China.
FAU - Li, Chaonan
AU  - Li C
AD  - Department of Rehabilitation Medicine, The First Affiliated Hospital of Xi'an 
      Medical University, Xi'an, Shaanxi 710077, P.R. China.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Rehabilitation Medicine, The First Affiliated Hospital of Xi'an 
      Medical University, Xi'an, Shaanxi 710077, P.R. China.
FAU - Lei, Lei
AU  - Lei L
AD  - Department of Rehabilitation Medicine, The First Affiliated Hospital of Xi'an 
      Medical University, Xi'an, Shaanxi 710077, P.R. China.
FAU - Zhang, Qiaojun
AU  - Zhang Q
AD  - Department of Nephrology, The Second Affiliated Hospital of Xi'an Jiaotong 
      University, Xi'an, Shaanxi 710004, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20191030
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6878885
OTO - NOTNLM
OT  - NF-kappaB
OT  - brain-derived neurotrophic factor
OT  - caspase 3
OT  - high-frequency electrotherapy
OT  - methylprednisolone
OT  - spinal cord injury
EDAT- 2019/12/07 06:00
MHDA- 2019/12/07 06:01
PMCR- 2019/10/30
CRDT- 2019/12/07 06:00
PHST- 2018/09/11 00:00 [received]
PHST- 2019/05/16 00:00 [accepted]
PHST- 2019/12/07 06:00 [entrez]
PHST- 2019/12/07 06:00 [pubmed]
PHST- 2019/12/07 06:01 [medline]
PHST- 2019/10/30 00:00 [pmc-release]
AID - ETM-0-0-8147 [pii]
AID - 10.3892/etm.2019.8147 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Dec;18(6):4682-4688. doi: 10.3892/etm.2019.8147. Epub 2019 Oct 
      30.