PMID- 31809637
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 2164-554X (Electronic)
IS  - 2164-5515 (Print)
IS  - 2164-5515 (Linking)
VI  - 16
IP  - 7
DP  - 2020 Jul 2
TI  - Polysaccharide PCP-I isolated from Poria cocos enhances the immunogenicity and 
      protection of an anthrax protective antigen-based vaccine.
PG  - 1699-1707
LID - 10.1080/21645515.2019.1675457 [doi]
AB  - Polysaccharides isolated from natural plants may represent a novel source of 
      vaccine adjuvants. In this research, we focused on a natural plant 
      polysaccharide, PCP-I, which is derived from Poria cocos, a Chinese traditional 
      herbal medicine. We chose the anthrax protective antigen (PA) as a model to 
      evaluate the adjuvant ability of PCP-I in enhancing the immunogenicity and 
      protection of a PA-based anthrax vaccine. According to our results, PCP-I could 
      significantly enhance anthrax specific anti-PA antibodies, toxin-neutralizing 
      antibodies, anti-PA antibody affinity, as well as IgG1 and IgG2a levels. Besides, 
      PCP-I increased the frequency of PA-specific memory B cells, increased the 
      proliferation of PA-specific splenocytes, significantly stimulated the secretion 
      of IL-4, and enhanced the activation of Dendritic cells (DCs) in vitro. The 
      combination of PCP-I and CpG significantly enhanced the level of anti-PA 
      antibodies and neutralizing antibodies, particularly PA-specific IgG2a, and 
      shifted the Th2-bias to a Th1/Th2 balanced response. In addition, PCP-I with or 
      without CpG could significantly improve the survival rate of immunized mice 
      following challenge with the anthrax lethal toxin. These findings suggest that 
      PCP-I may be a promising vaccine adjuvant that warrants further study.
FAU - Liu, Kun
AU  - Liu K
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Yin, Ying
AU  - Yin Y
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Zai, Xiaodong
AU  - Zai X
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Li, Ruihua
AU  - Li R
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Ma, Hao
AU  - Ma H
AD  - Institute of Pharmacology and Toxicology , Academy of Military Medical Sciences, 
      Beijing, China.
FAU - Xu, Junjie
AU  - Xu J
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
FAU - Shan, Junjie
AU  - Shan J
AD  - Institute of Pharmacology and Toxicology , Academy of Military Medical Sciences, 
      Beijing, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Laboratory of Vaccine and Antibody Engineering, Beijing Institute of 
      Biotechnology , Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191206
PL  - United States
TA  - Hum Vaccin Immunother
JT  - Human vaccines & immunotherapeutics
JID - 101572652
RN  - 0 (Anthrax Vaccines)
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Polysaccharides)
RN  - 0 (anthrax toxin)
SB  - IM
MH  - Animals
MH  - *Anthrax/prevention & control
MH  - *Anthrax Vaccines
MH  - Antibodies, Bacterial
MH  - Antigens, Bacterial
MH  - *Bacillus anthracis
MH  - Bacterial Toxins
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Polysaccharides
MH  - *Wolfiporia
PMC - PMC7482710
OTO - NOTNLM
OT  - Poria cocos
OT  - Polysaccharide
OT  - anthrax
OT  - protective antigen
OT  - vaccine adjuvant
EDAT- 2019/12/07 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/12/06
CRDT- 2019/12/07 06:00
PHST- 2019/12/07 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/12/07 06:00 [entrez]
PHST- 2020/12/06 00:00 [pmc-release]
AID - 1675457 [pii]
AID - 10.1080/21645515.2019.1675457 [doi]
PST - ppublish
SO  - Hum Vaccin Immunother. 2020 Jul 2;16(7):1699-1707. doi: 
      10.1080/21645515.2019.1675457. Epub 2019 Dec 6.