PMID- 31809738
OWN - NLM
STAT- MEDLINE
DCOM- 20200914
LR  - 20201207
IS  - 1875-9777 (Electronic)
IS  - 1934-5909 (Print)
IS  - 1875-9777 (Linking)
VI  - 25
IP  - 6
DP  - 2019 Dec 5
TI  - Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct 
      Functions and Fates in Skeletal Muscle Regeneration.
PG  - 797-813.e9
LID - S1934-5909(19)30459-X [pii]
LID - 10.1016/j.stem.2019.11.004 [doi]
AB  - Many adult tissues contain resident stem cells, such as the Pax7(+) satellite 
      cells within skeletal muscle, that regenerate parenchymal elements following 
      damage. Tissue-resident mesenchymal progenitors (MPs) also participate in 
      regeneration, although their function and fate in this process are unclear. Here, 
      we identify Hypermethylated in cancer 1 (Hic1) as a marker of MPs in skeletal 
      muscle and further show that Hic1 deletion leads to MP hyperplasia. Single-cell 
      RNA-seq and ATAC-seq analysis of Hic1(+) MPs in skeletal muscle shows multiple 
      subpopulations, which we further show have distinct functions and lineage 
      potential. Hic1(+) MPs orchestrate multiple aspects of skeletal muscle 
      regeneration by providing stage-specific immunomodulation and trophic and 
      mechanical support. During muscle regeneration, Hic1(+) derivatives directly 
      contribute to several mesenchymal compartments including Col22a1-expressing cells 
      within the myotendinous junction. Collectively, these findings demonstrate that 
      HIC1 regulates MP quiescence and identifies MP subpopulations with transient and 
      enduring roles in muscle regeneration.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Scott, R Wilder
AU  - Scott RW
AD  - Department of Cellular and Physiological Sciences, 2222 Health Sciences Mall, 
      University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; School of 
      Biomedical Engineering and the Biomedical Research Centre, 2222 Health Sciences 
      Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
FAU - Arostegui, Martin
AU  - Arostegui M
AD  - Department of Cellular and Physiological Sciences, 2222 Health Sciences Mall, 
      University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
FAU - Schweitzer, Ronen
AU  - Schweitzer R
AD  - Research Division, Shriners Hospital for Children, Portland, OR 97239, USA.
FAU - Rossi, Fabio M V
AU  - Rossi FMV
AD  - Department of Medical Genetics, 2222 Health Sciences Mall, University of British 
      Columbia, Vancouver, BC, V6T 1Z3, Canada; School of Biomedical Engineering and 
      the Biomedical Research Centre, 2222 Health Sciences Mall, University of British 
      Columbia, Vancouver, BC, V6T 1Z3, Canada.
FAU - Underhill, T Michael
AU  - Underhill TM
AD  - Department of Cellular and Physiological Sciences, 2222 Health Sciences Mall, 
      University of British Columbia, Vancouver, BC, V6T 1Z3, Canada; School of 
      Biomedical Engineering and the Biomedical Research Centre, 2222 Health Sciences 
      Mall, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada. Electronic 
      address: tunderhi@brc.ubc.ca.
LA  - eng
GR  - U54 CA231652/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Cell Stem Cell
JT  - Cell stem cell
JID - 101311472
RN  - 0 (Hic1 protein, mouse)
RN  - 0 (Kruppel-Like Transcription Factors)
SB  - IM
CIN - Cell Stem Cell. 2020 Feb 6;26(2):129-130. PMID: 32032520
MH  - Animals
MH  - Cell Cycle/genetics/physiology
MH  - Cell Differentiation/genetics/physiology
MH  - Cell Proliferation/genetics/physiology
MH  - Cells, Cultured
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Kruppel-Like Transcription Factors/genetics/*metabolism
MH  - Male
MH  - Mice
MH  - Muscle, Skeletal/*metabolism/*physiology
MH  - Regeneration/genetics/*physiology
MH  - Satellite Cells, Skeletal Muscle/*metabolism
MH  - Wound Healing/genetics/physiology
PMC - PMC6941576
MID - NIHMS1545049
OTO - NOTNLM
OT  - lineage tracing
OT  - mesenchymal progenitors
OT  - myotendinous junction
OT  - pericytes
OT  - quiescence
OT  - scATAC-seq
OT  - scRNA-seq
OT  - skeletal muscle
OT  - tendon
OT  - tissue regeneration
COIS- DECLARATION OF INTERESTS The authors have no competing interests to declare.
EDAT- 2019/12/07 06:00
MHDA- 2020/09/15 06:00
PMCR- 2020/12/05
CRDT- 2019/12/07 06:00
PHST- 2019/02/22 00:00 [received]
PHST- 2019/07/10 00:00 [revised]
PHST- 2019/11/11 00:00 [accepted]
PHST- 2019/12/07 06:00 [entrez]
PHST- 2019/12/07 06:00 [pubmed]
PHST- 2020/09/15 06:00 [medline]
PHST- 2020/12/05 00:00 [pmc-release]
AID - S1934-5909(19)30459-X [pii]
AID - 10.1016/j.stem.2019.11.004 [doi]
PST - ppublish
SO  - Cell Stem Cell. 2019 Dec 5;25(6):797-813.e9. doi: 10.1016/j.stem.2019.11.004.