PMID- 31810025 OWN - NLM STAT- MEDLINE DCOM- 20210823 LR - 20210823 IS - 1096-0023 (Electronic) IS - 1043-4666 (Linking) VI - 127 DP - 2020 Mar TI - Multiple cytokine analyses of aqueous humor from the patients with retinitis pigmentosa. PG - 154943 LID - S1043-4666(19)30372-2 [pii] LID - 10.1016/j.cyto.2019.154943 [doi] AB - PURPOSE: Cataracts are the most common eye complications of retinitis pigmentosa (RP). This study aimed to investigate the cytokine profiles of the aqueous humor of RP with cataracts. METHODS: The aqueous humor was collected from RP eyes with cataract (RP group, n = 20) and age-related cataract eyes (ARC group, n = 20) during cataract surgery. The levels of 37 mediators were measured with multiplex fluorescent bead-based immunoassay and compared across groups. The correlation among chemokines, growth factors, and cytokines was analyzed with Spearman's rank correlation coefficient. RESULTS: Twelve cytokines (IL-1alpha, IL-1beta, IL-4, IL-10, TNF-alpha, IFN-gamma, EGF, GM-CSF, PDGF-AB/BB, TGF-alpha, BMP-9, and E-selection) were below the limit of detection, and the detection rate of IL-6 was significantly higher in RP group than in the ARC group (P < 0.01). Compared with those in the control group, the aqueous humor levels of monocyte chemoattractant protein-1 (MCP-1), interleukin-(IL-)8, interferon gamma-induced protein (IP)-10, hepatocyte growth factor (HGF), platelet-derived growth factor AA (PDGF-AA), matrix metalloproteinase-2 (MMP-2), MMP3, MMP-7, MMP-8, plasminogen activator inhibitor-1 (PAI-1), and thrombospondin-2 (TSP-2) in the RP group increased significantly (P < 0.01). A lower level of BMP-4 in the aqueous humor was observed in the RP patients than in the controls (P < 0.05). CONCLUSIONS: Significantly increased levels of PDGF-AA, MMP2, MMP3, MMP-7, MMP-8, PAI-1, and TSP-2 and lower levels of BMP-4 were found in the aqueous humor of RP patients. This result indicates a disturbance of the extracellular matrix (ECM) and cytokines in RP patients and suggests a possible role of these cytokines in the pathogenesis of capsular contraction syndrome (CCS) in RP patients. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - Lu, Bing AU - Lu B AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Yin, Houfa AU - Yin H AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Tang, Qiaomei AU - Tang Q AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Wang, Wei AU - Wang W AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Luo, Chenqi AU - Luo C AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Chen, Xinyi AU - Chen X AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Zhang, Xiaobo AU - Zhang X AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Lai, Kairan AU - Lai K AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Xu, Jingjie AU - Xu J AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Chen, Xiangjun AU - Chen X AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. FAU - Yao, Ke AU - Yao K AD - Eye Center of the 2nd Affiliated Hospital, Medical College of Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Key Lab of Ophthalmology, Hangzhou, Zhejiang Province, China. Electronic address: xlren@zju.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191203 PL - England TA - Cytokine JT - Cytokine JID - 9005353 RN - 0 (Cytokines) RN - 0 (Platelet-Derived Growth Factor) RN - 0 (Transforming Growth Factor beta1) RN - 0 (Transforming Growth Factor beta2) RN - 0 (platelet-derived growth factor A) RN - EC 3.4.24.- (Matrix Metalloproteinases) SB - IM MH - Adult MH - Aged MH - Aqueous Humor/*metabolism MH - Cataract/complications/*metabolism MH - Cytokines/*metabolism MH - Female MH - Humans MH - Male MH - Matrix Metalloproteinases/metabolism MH - Middle Aged MH - Platelet-Derived Growth Factor/metabolism MH - Retinitis Pigmentosa/complications/*metabolism MH - Transforming Growth Factor beta1/metabolism MH - Transforming Growth Factor beta2/metabolism OTO - NOTNLM OT - Aqueous humor OT - Capsular contraction syndrome OT - Cytokine OT - Extracellular matrix OT - Retinitis pigmentosa COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2019/12/07 06:00 MHDA- 2021/08/24 06:00 CRDT- 2019/12/07 06:00 PHST- 2019/03/25 00:00 [received] PHST- 2019/11/21 00:00 [revised] PHST- 2019/11/22 00:00 [accepted] PHST- 2019/12/07 06:00 [pubmed] PHST- 2021/08/24 06:00 [medline] PHST- 2019/12/07 06:00 [entrez] AID - S1043-4666(19)30372-2 [pii] AID - 10.1016/j.cyto.2019.154943 [doi] PST - ppublish SO - Cytokine. 2020 Mar;127:154943. doi: 10.1016/j.cyto.2019.154943. Epub 2019 Dec 3.