PMID- 31811113
OWN - NLM
STAT- MEDLINE
DCOM- 20200521
LR  - 20200521
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Dec 7
TI  - Protective Effect of beta-Glucogallin on Damaged Cataract Against Methylglyoxal 
      Induced Oxidative Stress in Cultured Lens Epithelial Cells.
PG  - 9310-9318
LID - 10.12659/MSM.917869 [doi]
AB  - BACKGROUND ss-glucogallin (GG) is one of the major plant polyphenolic antioxidants 
      that have been associated with positive effects on human health and are crucial 
      in the developing defense mechanism against the risk of diseases. However, 
      reports on the protective mechanism of GG in lens epithelial cells are limited. 
      MATERIAL AND METHODS ARPE-19 cells (a human retinal epithelial cell line) were 
      exposed to methylglyoxal (MG) with or without GG to illuminate the protective 
      role of GG in counteracting the cataract signaling. RESULTS Cells predisposed to 
      MG demonstrated an increase in oxidative stress with augmented (P<0.01) 
      inflammatory cytokines such as cyclooxygenase (COX)-2, chemokine receptor CXCR4, 
      interleukin (IL)-6, IL-8, monocyte chemoattractant protein-1 (MCP-1), and 
      intercellular adhesion molecule 1 (ICAM-1) genes. In addition, the expression of 
      aldose reductase (AR) was increased to 2-fold with accumulated sorbitol in MG 
      exposed cells compared to control. On the other hand, cells exposed to MG 
      evidenced a 3-fold increase in RAGE (receptor for advanced glycation end 
      products) and a 2-fold increase in NF-kappaB (nuclear factor 
      kappa-light-chain-enhancer of activated B cells) expression compared to control 
      cells. Intriguingly, lens epithelial cells pre-treated with GG attenuated the 
      reactive oxygen species levels with improved antioxidant enzymes. Simultaneously, 
      the levels of AR and other inflammatory cytokines were observed in the levels 
      closer to control cells in GG pre-treated cells. CONCLUSIONS Thus, the results of 
      the present investigation show that GG may be a potential drug for the prevention 
      of cataract development and progression.
FAU - Ma, Ying
AU  - Ma Y
AD  - Department of Ophthalmology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China (mainland).
FAU - Liu, Fei
AU  - Liu F
AD  - Department of Ophthalmology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China (mainland).
FAU - Xu, Yanli
AU  - Xu Y
AD  - Department of Ophthalmology, The Second Hospital of Dalian Medical University, 
      Dalian, Liaoning, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20191207
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (Antioxidants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Hydrolyzable Tannins)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 58511-73-2 (glucogallin)
RN  - 722KLD7415 (Pyruvaldehyde)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Cataract/*drug therapy
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/metabolism
MH  - China
MH  - Cytokines/metabolism
MH  - Epithelial Cells/metabolism
MH  - Glycation End Products, Advanced
MH  - Humans
MH  - Hydrolyzable Tannins/metabolism/*pharmacology
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - Interleukin-6/metabolism
MH  - Lens, Crystalline/*drug effects/metabolism
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Oxidative Stress/drug effects/physiology
MH  - Pyruvaldehyde/pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
PMC - PMC6916131
COIS- Conflicts of interest None.
EDAT- 2019/12/08 06:00
MHDA- 2020/05/22 06:00
PMCR- 2019/12/07
CRDT- 2019/12/08 06:00
PHST- 2019/12/08 06:00 [entrez]
PHST- 2019/12/08 06:00 [pubmed]
PHST- 2020/05/22 06:00 [medline]
PHST- 2019/12/07 00:00 [pmc-release]
AID - 917869 [pii]
AID - 10.12659/MSM.917869 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Dec 7;25:9310-9318. doi: 10.12659/MSM.917869.