PMID- 31811221
OWN - NLM
STAT- MEDLINE
DCOM- 20201111
LR  - 20210110
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Dec 6
TI  - Mitigation of acyl-homoserine lactone (AHL) based bacterial quorum sensing, 
      virulence functions, and biofilm formation by yttrium oxide core/shell 
      nanospheres: Novel approach to combat drug resistance.
PG  - 18476
LID - 10.1038/s41598-019-53920-w [doi]
LID - 18476
AB  - The present study evaluated the efficacy of Y(2)O(3):Tb (core) and 
      Y(2)O(3):Tb@SiO(2) nanospheres (core/shell NSs) against virulence functions 
      regulated by quorum sensing (QS) and biofilm formation in pathogenic bacteria. 
      Scanning electron microscope (SEM) images were used to study the size, shape, and 
      morphology. The images clearly displayed spherical shaped, mono-dispersed 
      particles with narrow size distribution and an average grain size of 110-130 nm. 
      The chemical composition of the samples was determined by using energy dispersive 
      X-ray (EDX) and X-ray photoelectron spectroscopy (XPS). We determined the impact 
      of core and core/shell NSs on QS using sensor strains of Chromobacterium 
      violaceum CVO26 and Pseudomonas aeruginosa PAO1 in a comparative study. Sub-MICs 
      of core and core/shell NSs substantially suppressed QS-controlled violacein 
      production in C. violaceum. Similar concentration-dependent effect of sub-MICs of 
      synthesized core and core/shell NSs was observed in the QS-regulated virulence 
      functions (elastase, total protease, pyocyanin production, swarming motility, and 
      exopolysaccharide production) in PAO1. A concentration-dependent decrease 
      (14-60%) was recorded in the biofilm forming capability of PAO1, upon treatment 
      with core and core/shell NSs. Moreover, core/shell NSs were more effective in 
      inhibiting biofilm at higher tested concentrations as compared to core-NSs. The 
      synthesized NSs demonstrated significantly impaired attachment of cells to the 
      microtiter plate indicating that NSs target biofilm inhibition at the attachment 
      stage. Based on these results, we predict that core and core/shell NSs may be an 
      alternative to combat the threat of drug-resistant pathogenic bacteria.
FAU - Husain, Fohad Mabood
AU  - Husain FM
AD  - Department of Food Science and Nutrition, College of Food and Agriculture 
      Sciences, King Saud University, Riyadh, 11451, Saudi Arabia. fhusain@ksu.edu.sa.
FAU - Ansari, Anees A
AU  - Ansari AA
AD  - King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, 11451, 
      Saudi Arabia. amustaqeemahmad@ksu.edu.sa.
FAU - Khan, Aslam
AU  - Khan A
AD  - King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, 11451, 
      Saudi Arabia.
FAU - Ahmad, Naushad
AU  - Ahmad N
AD  - Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, 
      Saudi Arabia.
FAU - Albadri, Abdulrahman
AU  - Albadri A
AD  - National Center for Nanotechnology and Advanced Materials, King Abdulaziz City 
      for Science & Technology, Riyadh, 11442, Saudi Arabia.
FAU - Albalawi, Thamer H
AU  - Albalawi TH
AD  - Department of Biology, College of Science and Humanities, Prince Sattam bin 
      Abdulaziz University, Alkharj, 11942, Kingdom of Saudi Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191206
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Acyl-Butyrolactones)
RN  - 0 (Anti-Bacterial Agents)
RN  - 58784XQC3Y (Yttrium)
RN  - X8071685XT (yttria)
SB  - IM
MH  - *Acyl-Butyrolactones
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Biofilms/*drug effects/growth & development
MH  - Chromobacterium/drug effects/growth & development
MH  - Drug Resistance/*drug effects
MH  - Nanospheres
MH  - Pseudomonas aeruginosa/drug effects/growth & development
MH  - Quorum Sensing/*drug effects
MH  - Virulence/drug effects
MH  - *Yttrium
PMC - PMC6898131
COIS- The authors declare no competing interests.
EDAT- 2019/12/08 06:00
MHDA- 2020/11/12 06:00
PMCR- 2019/12/06
CRDT- 2019/12/08 06:00
PHST- 2019/05/21 00:00 [received]
PHST- 2019/10/28 00:00 [accepted]
PHST- 2019/12/08 06:00 [entrez]
PHST- 2019/12/08 06:00 [pubmed]
PHST- 2020/11/12 06:00 [medline]
PHST- 2019/12/06 00:00 [pmc-release]
AID - 10.1038/s41598-019-53920-w [pii]
AID - 53920 [pii]
AID - 10.1038/s41598-019-53920-w [doi]
PST - epublish
SO  - Sci Rep. 2019 Dec 6;9(1):18476. doi: 10.1038/s41598-019-53920-w.