PMID- 31811921
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 152
DP  - 2020 May 20
TI  - Protective effect of Prunella vulgaris var. L extract against blue light induced 
      damages in ARPE-19 cells and mouse retina.
PG  - 622-631
LID - S0891-5849(19)31257-2 [pii]
LID - 10.1016/j.freeradbiomed.2019.12.003 [doi]
AB  - Age-related macular degeneration (AMD) is one of leading causes that induce 
      severe visual impairment and loss in the elderly. Previous studies have suggested 
      that blue light (BL) could induce retinal degeneration, which is a major cause of 
      the onset and development of severe AMD. In the retinal pigment epithelium (RPE) 
      cells, A2E, a lipofuscin fluorophore, is accumulated with aging. When A2E is 
      exposed to BL, it is easily oxidized to A2E-epoxides, leading to oxidative stress 
      and inflammatory response in retina. The aim of this study was to investigate 
      protective effect of Prunella vulagris (P.V) extract against oxidative stress and 
      inflammation caused by BL, and to elucidate the underlying mechanisms in the 
      cultured RPE cells and balb-c mice. In both model studies, P.V extract activated 
      NF-E2 related factor 2 (Nrf-2)/hemeoxygenase-1 (HO-1) signaling pathway, followed 
      by inhibition of ROS/MDA production, GSH depletion and reduction in SOD activity. 
      Furthermore, P.V extract inhibited upregulation of inflammatory related genes 
      (interlukin (IL)-1beta, IL-6, monocyte chemoattractant protein-1 (MCP-1), 
      vascular endothelial growth factor A (VEGF A)) and BL induced RPE cell death, 
      determined by cell viability and histological analyses. The mechanism of 
      protection against inflammation by P.V extract involves inhibition of nuclear 
      translocation of nuclear factor kappa beta (NF-kB) along with degradation of 
      NF-kB inhibitor alpha (IkB alpha). The results suggest that P.V extract could be 
      a potential intervention to prevent the onset and development of severe AMD.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Kim, Jun
AU  - Kim J
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea. 
      Electronic address: Jun2@khu.ac.kr.
FAU - Cho, Kyoungwon
AU  - Cho K
AD  - Chong Kun Dang Healthcare Corporation, 47, Beodeunaru-ro, Yeongdeungpo-gu, Seoul, 
      07249, Republic of Korea.
FAU - Choung, Se-Young
AU  - Choung SY
AD  - Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee 
      University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, Republic of Korea. 
      Electronic address: sychoung@khu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20191205
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Plant Extracts)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Light
MH  - Oxidative Stress
MH  - Plant Extracts/pharmacology
MH  - *Prunella
MH  - Retina
MH  - *Retinal Pigment Epithelium
MH  - Vascular Endothelial Growth Factor A
OTO - NOTNLM
OT  - Age-related macular degeneration
OT  - Blue light
OT  - Inflammation
OT  - Oxidative stress
OT  - Prunella vulgaris
OT  - Retinal degeneration
EDAT- 2019/12/08 06:00
MHDA- 2021/06/22 06:00
CRDT- 2019/12/08 06:00
PHST- 2019/07/29 00:00 [received]
PHST- 2019/11/19 00:00 [revised]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2019/12/08 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/12/08 06:00 [entrez]
AID - S0891-5849(19)31257-2 [pii]
AID - 10.1016/j.freeradbiomed.2019.12.003 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2020 May 20;152:622-631. doi: 
      10.1016/j.freeradbiomed.2019.12.003. Epub 2019 Dec 5.