PMID- 31812810
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210110
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Linking)
VI  - 22
DP  - 2019 Dec 20
TI  - High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium 
      Ion Indicators.
PG  - 400-408
LID - S2589-0042(19)30484-5 [pii]
LID - 10.1016/j.isci.2019.11.034 [doi]
AB  - Functional optoacoustic (OA) imaging assisted with genetically encoded calcium 
      ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at 
      depths and spatiotemporal resolutions not attainable with existing optical 
      microscopic techniques. However, currently available GECIs optimized for 
      fluorescence (FL) imaging lack sufficient contrast for OA imaging and respond at 
      wavelengths having limited penetration into the mammalian brain. Here we present 
      an imaging platform capable of rapid assessment and cross-validation between OA 
      and FL responses of sensor proteins expressed in Escherichia coli colonies. The 
      screening system features optimized pulsed light excitation combined with 
      ultrasensitive ultrasound detection to mitigate photobleaching while further 
      allowing the dynamic characterization of calcium ion responses with millisecond 
      precision. Targeted probing of up to six individual colonies per second in both 
      calcium-loaded and calcium-unloaded states was possible with the system. The new 
      platform greatly facilitates optimization of absorption-based labels, thus 
      setting the stage for directed evolution of OA GECIs.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Hofmann, Urs A T
AU  - Hofmann UAT
AD  - Institute of Pharmacology and Toxicology and Faculty of Medicine, University of 
      Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Institute for 
      Biomedical Engineering and Department of Information Technology and Electrical 
      Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
FAU - Fabritius, Arne
AU  - Fabritius A
AD  - Tools for Bio-Imaging, Max Planck Institute, Am Klopferspitz 18, 82152 
      Martinsried, Germany.
FAU - Rebling, Johannes
AU  - Rebling J
AD  - Institute of Pharmacology and Toxicology and Faculty of Medicine, University of 
      Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Institute for 
      Biomedical Engineering and Department of Information Technology and Electrical 
      Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
FAU - Estrada, Hector
AU  - Estrada H
AD  - Institute of Pharmacology and Toxicology and Faculty of Medicine, University of 
      Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Institute for 
      Biomedical Engineering and Department of Information Technology and Electrical 
      Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
FAU - Dean-Ben, X Luis
AU  - Dean-Ben XL
AD  - Institute of Pharmacology and Toxicology and Faculty of Medicine, University of 
      Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Institute for 
      Biomedical Engineering and Department of Information Technology and Electrical 
      Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.
FAU - Griesbeck, Oliver
AU  - Griesbeck O
AD  - Tools for Bio-Imaging, Max Planck Institute, Am Klopferspitz 18, 82152 
      Martinsried, Germany.
FAU - Razansky, Daniel
AU  - Razansky D
AD  - Institute of Pharmacology and Toxicology and Faculty of Medicine, University of 
      Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland; Institute for 
      Biomedical Engineering and Department of Information Technology and Electrical 
      Engineering, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland. Electronic 
      address: daniel.razansky@uzh.ch.
LA  - eng
PT  - Journal Article
DEP - 20191125
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC6911978
OTO - NOTNLM
OT  - Analytical Chemistry
OT  - Bioengineering
OT  - Biomaterials
COIS- The authors declare no competing interests.
EDAT- 2019/12/10 06:00
MHDA- 2019/12/10 06:01
PMCR- 2019/11/25
CRDT- 2019/12/09 06:00
PHST- 2019/04/15 00:00 [received]
PHST- 2019/11/15 00:00 [revised]
PHST- 2019/11/19 00:00 [accepted]
PHST- 2019/12/10 06:00 [pubmed]
PHST- 2019/12/10 06:01 [medline]
PHST- 2019/12/09 06:00 [entrez]
PHST- 2019/11/25 00:00 [pmc-release]
AID - S2589-0042(19)30484-5 [pii]
AID - 10.1016/j.isci.2019.11.034 [doi]
PST - ppublish
SO  - iScience. 2019 Dec 20;22:400-408. doi: 10.1016/j.isci.2019.11.034. Epub 2019 Nov 
      25.