PMID- 31814902
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 11
IP  - 11
DP  - 2019
TI  - Extracellular vesicles from human umbilical cord mesenchymal stem cells treated 
      with siRNA against ELFN1-AS1 suppress colon adenocarcinoma proliferation and 
      migration.
PG  - 6989-6999
AB  - Recent evidence has shown that long noncoding RNAs (lncRNAs) play major roles in 
      tumorigenesis and cancer progression. The cancer genome atlas program (TCGA) 
      database was used to screen colon adenocarcinoma (COAD)-related differentially 
      expressed lncRNAs, which revealed that lncRNA ELFN1-AS1 was highly expressed in 
      COAD. This study aimed to explore the regulatory role of ELFN1-AS1 in COAD and 
      construct a gene delivery system based on extracellular vesicles (EVs). We found 
      that ELFN1-AS1 levels were obviously increased in COAD patients and COAD tumor 
      cells. Knockdown of ELFN1-AS1 expression by siRNA inhibited COAD cell 
      proliferation and migration. Moreover, silencing ELFN1-AS1 significantly reduced 
      the activation of extracellular signal-regulated protein kinase (Erk), 
      up-regulated the protein expression of E-cadherin and down-regulated vimentin. In 
      addition, we treated human umbilical cord mesenchymal stem cells (hUCMSCs) with 
      siRNA-ELFN1-AS1 and found that EVs from siRNA-ELFN1-AS1-treated hUCMSCs could 
      inhibit COAD cell proliferation and migration in vitro. These findings suggested 
      that ELFN1-AS1 could promote the progression of COAD and that hUCMSC-EVs might be 
      an attractive vehicle for the clinical administration of lncRNA-specific siRNAs 
      in patients with COAD.
CI  - AJTR Copyright (c) 2019.
FAU - Dong, Liyang
AU  - Dong L
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Ding, Chao
AU  - Ding C
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Zheng, Tingting
AU  - Zheng T
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Pu, Yanan
AU  - Pu Y
AD  - State Key Lab of Reproductive Medicine, Jiangsu Key Laboratory of Pathogen 
      Biology, Department of Pathogen Biology and Immunology, Nanjing Medical 
      University Nanjing 211166, Jiangsu, China.
FAU - Liu, Jiameng
AU  - Liu J
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Zhang, Wenzhe
AU  - Zhang W
AD  - Department of Central Laboratory, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Xue, Fei
AU  - Xue F
AD  - Department of Central Laboratory, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Kang, Ping
AU  - Kang P
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Ma, Yongbin
AU  - Ma Y
AD  - Department of Neurology Laboratory, Jintan Hospital, Jiangsu University Jintan 
      213200, Jiangsu, China.
FAU - Wang, Xuefeng
AU  - Wang X
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
AD  - Department of Central Laboratory, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
FAU - Mao, Chaoming
AU  - Mao C
AD  - Department of Nuclear Medicine, The Affiliated Hospital of Jiangsu University 
      Zhenjiang 212000, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20191115
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC6895508
OTO - NOTNLM
OT  - ELFN1-AS1
OT  - colon adenocarcinoma
OT  - extracellular vesicles
OT  - human umbilical cord mesenchymal stem cells
COIS- None.
EDAT- 2019/12/10 06:00
MHDA- 2019/12/10 06:01
PMCR- 2019/11/15
CRDT- 2019/12/10 06:00
PHST- 2019/08/01 00:00 [received]
PHST- 2019/10/29 00:00 [accepted]
PHST- 2019/12/10 06:00 [entrez]
PHST- 2019/12/10 06:00 [pubmed]
PHST- 2019/12/10 06:01 [medline]
PHST- 2019/11/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2019 Nov 15;11(11):6989-6999. eCollection 2019.