PMID- 31815137
OWN - NLM
STAT- MEDLINE
DCOM- 20200420
LR  - 20200420
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2019
DP  - 2019
TI  - Identification of Circular RNAs Regulating Islet beta-Cell Autophagy in Type 2 
      Diabetes Mellitus.
PG  - 4128315
LID - 10.1155/2019/4128315 [doi]
LID - 4128315
AB  - This study is to identify the circular RNA (circRNA) expression profile that is 
      functionally related to pancreatic islet beta-cell autophagy and their potential 
      regulation mechanisms in type 2 diabetes mellitus (T2DM). T2DM rat model was 
      constructed by administration of high-fat and high-sugar diet. beta-cells were 
      isolated from islets by flow cytometry. CircRNA expression profile in beta-cells was 
      detected by circRNA microarrays, and the differentially expressed circRNAs were 
      identified and validated by qRT-PCR. MicroRNA (miRNA) target prediction software 
      and multiple bioinformatic approaches were used to construct a map of 
      circRNA-miRNA interactions for the differentially expressed circRNAs. A total of 
      825 differentially expressed circular transcripts were identified in T2DM rats 
      compared with control rats, among which 388 were upregulated and 437 were 
      downregulated. Ten circRNAs were identified to have significant differences by 
      qRT-PCR. GO analysis enriched terms such as organelle membrane and protein 
      binding and the top enriched pathways for the circRNAs included MAPK signaling 
      pathway. The differentially expressed circRNAs might involve in MAPK signaling 
      pathway, apoptosis, and Ras signaling pathway. We speculate that these circRNAs, 
      especially rno_circRNA_008565, can regulate the autophagy of islet beta-cells via 
      interactions with miRNA. Dysregulation of several circRNAs may play a role in 
      T2DM development, and rno_circRNA_008565 may be a potential regulator of beta-cell 
      autophagy.
CI  - Copyright (c) 2019 Chao Bai et al.
FAU - Bai, Chao
AU  - Bai C
AUID- ORCID: 0000-0002-6507-4954
AD  - Department of Vascular Thyroid Surgery, First Hospital Affiliated to Xinjiang 
      Medical University, Urumqi 830054, China.
FAU - Yang, Wenwen
AU  - Yang W
AUID- ORCID: 0000-0002-0367-0128
AD  - Department of Endocrinology, Second Hospital Affiliated to Xinjiang Medical 
      University, Urumqi 830063, China.
FAU - Lu, Yao
AU  - Lu Y
AD  - Department of Endocrinology, Second Hospital Affiliated to Xinjiang Medical 
      University, Urumqi 830063, China.
FAU - Wei, Wei
AU  - Wei W
AUID- ORCID: 0000-0002-8678-0989
AD  - Department of Endocrinology, Second Hospital Affiliated to Xinjiang Medical 
      University, Urumqi 830063, China.
FAU - Li, Zongbao
AU  - Li Z
AUID- ORCID: 0000-0001-6755-3038
AD  - Department of Endocrinology, Sanya Central Hospital, Sanya 572000, China.
FAU - Zhang, Li
AU  - Zhang L
AUID- ORCID: 0000-0003-2028-8243
AD  - Department of Endocrinology, Sanya Central Hospital, Sanya 572000, China.
LA  - eng
PT  - Journal Article
DEP - 20191107
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Animals
MH  - Autophagy/genetics/*physiology
MH  - Computational Biology
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Diet, High-Fat/adverse effects
MH  - Dietary Carbohydrates/adverse effects
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Regulatory Networks
MH  - Islets of Langerhans/*metabolism
MH  - Male
MH  - MicroRNAs/metabolism
MH  - Protein Binding
MH  - RNA, Circular/genetics/*isolation & purification/*metabolism
MH  - Rats
MH  - Signal Transduction
MH  - Transcriptome
MH  - Up-Regulation
PMC - PMC6878796
COIS- The authors declare that there are no conflicts of interest regarding the 
      publication of this paper.
EDAT- 2019/12/10 06:00
MHDA- 2020/04/21 06:00
PMCR- 2019/11/07
CRDT- 2019/12/10 06:00
PHST- 2019/05/23 00:00 [received]
PHST- 2019/08/30 00:00 [revised]
PHST- 2019/09/29 00:00 [accepted]
PHST- 2019/12/10 06:00 [entrez]
PHST- 2019/12/10 06:00 [pubmed]
PHST- 2020/04/21 06:00 [medline]
PHST- 2019/11/07 00:00 [pmc-release]
AID - 10.1155/2019/4128315 [doi]
PST - epublish
SO  - Biomed Res Int. 2019 Nov 7;2019:4128315. doi: 10.1155/2019/4128315. eCollection 
      2019.