PMID- 31817065
OWN - NLM
STAT- MEDLINE
DCOM- 20200420
LR  - 20200420
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 24
DP  - 2019 Dec 4
TI  - Identification of B Cell Epitopes of Blo t 13 Allergen and Cross-Reactivity with 
      Human Adipocytes and Heart Fatty Acid Binding Proteins.
LID - 10.3390/ijms20246107 [doi]
LID - 6107
AB  - Cross-reactivity between allergens and human proteins could have a clinical 
      impact in allergic diseases. Blo t 13 is an allergen from the mite Blomia 
      tropicalis, which belongs to the fatty acid binding protein (FABP) family and has 
      structural homology with human FABPs. This work aimed to map B cell epitopes on 
      Blo t 13 and to identify epitopes involved in cross-reactivity with human heart 
      FABP (FABP3) and adipocyte FABP (FABP4). Sera from 25 patients with house dust 
      mite (HDM) allergy that were sensitized to Blo t 13 were used for testing the 
      reactivity of immunoglobulin E (IgE) and IgG to FABP. The epitope mapping of Blo 
      t 13 was performed using overlapping peptides, and cross-reactivity between Blo t 
      13 and human FABP was analyzed using human sera and anti-Blo t 13 monoclonal 
      antibodies. IgE antibodies to all FABPs were detected in 14/25 serum samples, and 
      IgG was detected in 25/25 serum samples. The cross-reactivity of Blo t 13 was 42% 
      with FABP3 and 48% with FABP4. Two IgE-binding regions were identified in Blo t 
      13; one between residues 54 and 72 (the main cross-reacting region) and another 
      between residues 111 to 129. Our results suggest that exposure to the Blo t 13 
      allergen could induce an auto-reactive response to endogenous FABP in allergic 
      patients sensitized to Blo t 13.
FAU - Munera, Marlon
AU  - Munera M
AD  - Institute for Immunological Research, University of Cartagena, Cartagena 130000, 
      Colombia.
FAU - Martinez, Dalgys
AU  - Martinez D
AD  - Institute for Immunological Research, University of Cartagena, Cartagena 130000, 
      Colombia.
FAU - Labrada, Alexis
AU  - Labrada A
AUID- ORCID: 0000-0003-0956-7946
AD  - Centro Nacional de Biopreparados (BIOCEN), Bejucal 32600, Cuba.
FAU - Caraballo, Luis
AU  - Caraballo L
AD  - Institute for Immunological Research, University of Cartagena, Cartagena 130000, 
      Colombia.
FAU - Puerta, Leonardo
AU  - Puerta L
AUID- ORCID: 0000-0003-2246-9697
AD  - Institute for Immunological Research, University of Cartagena, Cartagena 130000, 
      Colombia.
LA  - eng
GR  - Grant 368-2011/Colciencias and University of Cartagena, Cartagena. Colombia/
PT  - Journal Article
DEP - 20191204
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Allergens)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Blo t 13 allergen)
RN  - 0 (Epitopes, B-Lymphocyte)
RN  - 0 (FABP3 protein, human)
RN  - 0 (FABP4 protein, human)
RN  - 0 (Fatty Acid Binding Protein 3)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adipocytes/metabolism
MH  - Allergens/genetics/immunology/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Cross Reactions
MH  - Epitope Mapping
MH  - Epitopes, B-Lymphocyte/*immunology
MH  - Fatty Acid Binding Protein 3/chemistry/genetics/*immunology
MH  - Fatty Acid-Binding Proteins/chemistry/genetics/*immunology/*metabolism
MH  - Female
MH  - Humans
MH  - Hypersensitivity/blood/pathology
MH  - Immunoglobulin E/immunology
MH  - Immunoglobulin G/immunology
MH  - Male
MH  - Mites/metabolism
MH  - Myocardium/metabolism
MH  - Protein Structure, Tertiary
MH  - Sequence Alignment
PMC - PMC6940925
OTO - NOTNLM
OT  - Blomia tropicalis
OT  - FABP
OT  - IgE
OT  - IgG
OT  - cross-reactivity
OT  - house dust mites
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/11 06:00
MHDA- 2020/04/21 06:00
PMCR- 2019/12/01
CRDT- 2019/12/11 06:00
PHST- 2019/10/21 00:00 [received]
PHST- 2019/11/29 00:00 [revised]
PHST- 2019/11/29 00:00 [accepted]
PHST- 2019/12/11 06:00 [entrez]
PHST- 2019/12/11 06:00 [pubmed]
PHST- 2020/04/21 06:00 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - ijms20246107 [pii]
AID - ijms-20-06107 [pii]
AID - 10.3390/ijms20246107 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Dec 4;20(24):6107. doi: 10.3390/ijms20246107.