PMID- 31818667 OWN - NLM STAT- MEDLINE DCOM- 20201231 LR - 20201231 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 388 DP - 2020 Apr 15 TI - Exposure to acrylamide inhibits uterine decidualization via suppression of cyclin D3/p21 and apoptosis in mice. PG - 121785 LID - S0304-3894(19)31739-X [pii] LID - 10.1016/j.jhazmat.2019.121785 [doi] AB - Acrylamide (ACR), a neurotoxicity and carcinogenic chemical, has attracted considerable attention since it is present at high concentrations in thermally cooked carbohydrate-rich foods. ACR exposure significantly increased rate of fetal resorption, and decreased fetal body weights in mice. However, no detailed information is available about the effect of ACR on uterine decidualization, which is a vital process in the establishment of successful pregnancy. Thus, our aim of this study was to explore the effect and mechanism of ACR on uterine decidualization in vivo during mice pregnancy. Mice were gavaged with 0, 10, and 50 mg ACR /kg/day from gestational days (GD) 1 until GD 8, whereas pseudopregnant mice from pseudopregnant day (PPD) 4 until PPD 8. Results indicated ACR treatment dramatically reduced numbers of implanted embryos, and decreased the weights of implantation site and oil-induced uterus. Nevertheless, no significant difference was observed in the weights of no oil-induced uterus between control and ACR-treated group. Furthermore, ACR significantly reduced numbers of polyploidy and PCNA-positive decidual cells and expression of cyclin D3 and p21 proteins, and induced apoptosis of decidua, as presented by up-regulation of Bax and cleaved-caspase-3, and decreased Bcl-2 protein during normal pregnant and pseudopregnant process. In summary, ACR exposure significantly inhibited uterine endometrial decidualization via the apoptosis and suppression of cyclin D3/p21 in mice. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Yu, Dainan AU - Yu D AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: 1747060467@qq.com. FAU - Liu, Qingyun AU - Liu Q AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China; The Second Clinical Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: 1277587270@qq.com. FAU - Qiao, Bo AU - Qiao B AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: qiaoxiaogu@163.com. FAU - Jiang, Wenyu AU - Jiang W AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: 275560237@qq.com. FAU - Zhang, Lixia AU - Zhang L AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Gynecology and Children, Jiaxing University, Jiaxing, Zhejiang, 314001, PR China. Electronic address: 1257648202@qq.com. FAU - Shen, Xin AU - Shen X AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: 1694915656@qq.com. FAU - Xie, Liping AU - Xie L AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: 1433115927@qq.com. FAU - Liu, Hui AU - Liu H AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: hhcomeon@126.com. FAU - Zhang, Dalei AU - Zhang D AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: zhangdalei@ncu.edu.cn. FAU - Yang, Bei AU - Yang B AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: yangbei@ncu.edu.cn. FAU - Kuang, Haibin AU - Kuang H AD - Department of Physiology, Basic Medical College, Nanchang University, Nanchang, Jiangxi, 330006, PR China; Jiangxi Provincial Key Laboratory of Reproductive Physiology and Pathology, Medical Experimental Teaching Center, Nanchang University, Nanchang, Jiangxi, 330006, PR China. Electronic address: kuanghaibin@ncu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191128 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 RN - 0 (Cyclin D3) RN - 0 (Cyclin-Dependent Kinase Inhibitor p21) RN - 20R035KLCI (Acrylamide) SB - IM MH - Acrylamide/*toxicity MH - Animals MH - Apoptosis/drug effects MH - Cyclin D3/*metabolism MH - Cyclin-Dependent Kinase Inhibitor p21/*metabolism MH - Down-Regulation MH - Embryo Implantation MH - Female MH - Mice MH - Pregnancy MH - Uterus/*drug effects/growth & development/metabolism OTO - NOTNLM OT - Acrylamide OT - Apoptosis OT - Decidualization OT - Pregnancy OT - Reproduction COIS- Declaration of Competing Interest The authors declared that there are no conflicts of interests of this work. EDAT- 2019/12/11 06:00 MHDA- 2021/01/01 06:00 CRDT- 2019/12/11 06:00 PHST- 2019/08/05 00:00 [received] PHST- 2019/11/25 00:00 [revised] PHST- 2019/11/27 00:00 [accepted] PHST- 2019/12/11 06:00 [pubmed] PHST- 2021/01/01 06:00 [medline] PHST- 2019/12/11 06:00 [entrez] AID - S0304-3894(19)31739-X [pii] AID - 10.1016/j.jhazmat.2019.121785 [doi] PST - ppublish SO - J Hazard Mater. 2020 Apr 15;388:121785. doi: 10.1016/j.jhazmat.2019.121785. Epub 2019 Nov 28.