PMID- 31818980
OWN - NLM
STAT- MEDLINE
DCOM- 20200810
LR  - 20200810
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 204
IP  - 2
DP  - 2020 Jan 15
TI  - Borrelia miyamotoi Activates Human Dendritic Cells and Elicits T Cell Responses.
PG  - 386-393
LID - 10.4049/jimmunol.1801589 [doi]
AB  - The spirochete Borrelia miyamotoi has recently been shown to cause relapsing 
      fever. Like the Lyme disease agent, Borrelia burgdorferi, B. miyamotoi is 
      transmitted through the bite of infected ticks; however, little is known about 
      the response of the immune system upon infection. Dendritic cells (DCs) play a 
      central role in the early immune response against B. burgdorferi We investigated 
      the response of DCs to two different strains of B. miyamotoi using in vitro and 
      ex vivo models and compared this to the response elicited by B. burgdorferi. Our 
      findings show that B. miyamotoi is phagocytosed by monocyte-derived DCs, causing 
      upregulation of activation markers and production of proinflammatory cytokines in 
      a similar manner to B. burgdorferi. Recognition of B. miyamotoi was demonstrated 
      to be partially mediated by TLR2. DCs migrated out of human skin explants upon 
      inoculation of the skin with B. miyamotoi. Finally, we showed that B. 
      miyamotoi-stimulated DCs induced proliferation of naive CD4(+) and CD8(+) T cells 
      to a larger extent than B. burgdorferi. In conclusion, we show in this study that 
      DCs respond to and mount an immune response against B. miyamotoi that is similar 
      to the response to B. burgdorferi and is able to induce T cell proliferation.
CI  - Copyright (c) 2020 by The American Association of Immunologists, Inc.
FAU - Mason, Lauren M K
AU  - Mason LMK
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands; l.m.mason@amc.uva.nl.
FAU - Koetsveld, Joris
AU  - Koetsveld J
AUID- ORCID: 0000-0002-4604-5111
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Trentelman, Jos J A
AU  - Trentelman JJA
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Kaptein, Tanja M
AU  - Kaptein TM
AD  - Department of Experimental Immunology, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Hoornstra, Dieuwertje
AU  - Hoornstra D
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Wagemakers, Alex
AU  - Wagemakers A
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Fikrig, Michelle M
AU  - Fikrig MM
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Ersoz, Jasmin I
AU  - Ersoz JI
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Oei, Anneke
AU  - Oei A
AD  - Department of Medical Microbiology, Amsterdam Infection and Immunity, Amsterdam 
      University Medical Center, University of Amsterdam, 1105AZ Amsterdam, the 
      Netherlands; and.
FAU - Geijtenbeek, Teunis B H
AU  - Geijtenbeek TBH
AD  - Department of Experimental Immunology, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
FAU - Hovius, Joppe W R
AU  - Hovius JWR
AD  - Center for Experimental and Molecular Medicine, Amsterdam Infection and Immunity, 
      Amsterdam University Medical Center, University of Amsterdam, 1105AZ Amsterdam, 
      the Netherlands.
AD  - Division of Infectious Diseases, Amsterdam Multidisciplinary Lyme Borreliosis 
      Center, Department of Internal Medicine, Amsterdam University Medical Center, 
      University of Amsterdam, 1105AZ Amsterdam, the Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191209
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (TLR2 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - Borrelia miyamotoi
SB  - IM
MH  - Animals
MH  - Borrelia/*physiology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Insect Bites and Stings/*immunology
MH  - Lymphocyte Activation
MH  - Phagocytosis
MH  - Relapsing Fever/*immunology
MH  - Skin/*pathology
MH  - T-Lymphocytes/*immunology
MH  - Ticks/*immunology/microbiology
MH  - Toll-Like Receptor 2/metabolism
EDAT- 2019/12/11 06:00
MHDA- 2020/08/11 06:00
CRDT- 2019/12/11 06:00
PHST- 2018/12/17 00:00 [received]
PHST- 2019/11/07 00:00 [accepted]
PHST- 2019/12/11 06:00 [pubmed]
PHST- 2020/08/11 06:00 [medline]
PHST- 2019/12/11 06:00 [entrez]
AID - jimmunol.1801589 [pii]
AID - 10.4049/jimmunol.1801589 [doi]
PST - ppublish
SO  - J Immunol. 2020 Jan 15;204(2):386-393. doi: 10.4049/jimmunol.1801589. Epub 2019 
      Dec 9.