PMID- 31819766
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 18
IP  - 6
DP  - 2019 Dec
TI  - Clinical, microbiological and oxidative stress evaluation of periodontitis 
      patients treated with two regimens of systemic antibiotics, adjunctive to 
      non-surgical therapy: A placebo-controlled randomized clinical trial.
PG  - 5001-5015
LID - 10.3892/etm.2019.7856 [doi]
AB  - Aim of research was to evaluate and compare the clinical and microbiological 
      effects of two different regimens of amoxicillin (AMX) and metronidazole (MTZ) 
      combined with non-surgical periodontal therapy in patients with chronic 
      periodontitis (CP), and identify antibiotic-resistant bacteria and changes in 
      oxidative stress (OS). Forty-six patients with generalized CP were randomly 
      assigned to group A [scaling and root planing within 24 h (SRP) + placebo for 7 
      days), group B [SRP + AMX + MTZ, both 500 mg three times daily (TID), 3 days), 
      and group C [SRP + AMX + MTZ, both 500 mg TID, 7 days). Periodontal pocket depth 
      (PPD-primary outcome), clinical attachment level (CAL), full-mouth bleeding 
      scores (FMBS), plaque scores (FMPS), blood and subgingival plaque were assessed 
      at baseline and after three months. OS was evaluated via derivatives of reactive 
      oxygen metabolites (d-ROMs) and assessments of biological antioxidant potential 
      (BAP). Bacterial profiling was performed by PCR. Antibiotic resistance was 
      evaluated in cultures. PPD, CAL, number of sites with PPD >/=6 mm, their PPD, CAL 
      and FMBS decreased (P<0.05) in all groups, as well as FMPS in groups A and B, and 
      d-ROMs in group C. There were significant differences among groups regarding 
      decreases in the frequency of detection for Aa and Tf. For Aa, there were 
      differences between groups A and C (P=0.048) and between groups B and C 
      (P=0.048), but not between groups A and B; whereas for Tf, groups A and B were 
      different from group C (P<0.001), but not from each other (P=0.920). No 
      resistance to AMX was identified prior to treatment; two strains were resistant 
      after treatment. Before treatment, 13 strains were resistant to MTZ, and 2 were 
      resistant after. One strain in the same patient was sensitive prior to treatment, 
      and later became resistant to both antibiotics. SRP with a 7-day course of 
      antibiotic therapy was more effective for improving clinical parameters, in 
      decrease of detection of several periopathogens, and in improvement of OS when 
      compared to a 3-day regimen. Resistance was found in fewer strains after 
      treatment than before.
CI  - Copyright: (c) Boia et al.
FAU - Boia, Simina
AU  - Boia S
AD  - Department of Periodontology, Faculty of Dental Medicine, 'Victor Babes' 
      University of Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Boariu, Marius
AU  - Boariu M
AD  - Department of Endodontics, Faculty of Dental Medicine, 'Victor Babes' University 
      of Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Baderca, Flavia
AU  - Baderca F
AD  - Department of Histology, Faculty of Medicine, 'Victor Babes' University of 
      Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Rusu, Darian
AU  - Rusu D
AD  - Department of Periodontology, Faculty of Dental Medicine, 'Victor Babes' 
      University of Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Muntean, Delia
AU  - Muntean D
AD  - Department of Microbiology, Faculty of Medicine, 'Victor Babes' University of 
      Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Horhat, Florin
AU  - Horhat F
AD  - Department of Microbiology, Faculty of Medicine, 'Victor Babes' University of 
      Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Boia, Eugen-Radu
AU  - Boia ER
AD  - Department of ENT, Faculty of Medicine, 'Victor Babes' University of Medicine and 
      Pharmacy, 300041 Timisoara, Romania.
FAU - Borza, Claudia
AU  - Borza C
AD  - Department of Pathophysiology, Faculty of Medicine, 'Victor Babes' University of 
      Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Anghel, Andrei
AU  - Anghel A
AD  - Department of Biochemistry, Faculty of Medicine, 'Victor Babes' University of 
      Medicine and Pharmacy, 300041 Timisoara, Romania.
FAU - Stratul, Stefan-Ioan
AU  - Stratul SI
AD  - Department of Periodontology, Faculty of Dental Medicine, 'Victor Babes' 
      University of Medicine and Pharmacy, 300041 Timisoara, Romania.
LA  - eng
PT  - Journal Article
DEP - 20190805
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6895779
OTO - NOTNLM
OT  - antibiotic resistance
OT  - antibiotics
OT  - bacteria
OT  - oxidative stress
OT  - periodontitis
OT  - root planing
EDAT- 2019/12/11 06:00
MHDA- 2019/12/11 06:01
PMCR- 2019/08/05
CRDT- 2019/12/11 06:00
PHST- 2019/05/30 00:00 [received]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/12/11 06:00 [entrez]
PHST- 2019/12/11 06:00 [pubmed]
PHST- 2019/12/11 06:01 [medline]
PHST- 2019/08/05 00:00 [pmc-release]
AID - ETM-0-0-7856 [pii]
AID - 10.3892/etm.2019.7856 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Dec;18(6):5001-5015. doi: 10.3892/etm.2019.7856. Epub 2019 Aug 
      5.