PMID- 31821819
OWN - NLM
STAT- MEDLINE
DCOM- 20210528
LR  - 20210528
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 277
DP  - 2020 Mar
TI  - Development and application of a combined molecular and tissue culture-based 
      approach to detect latent infectious laryngotracheitis virus (ILTV) in chickens.
PG  - 113797
LID - S0166-0934(19)30380-5 [pii]
LID - 10.1016/j.jviromet.2019.113797 [doi]
AB  - Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in 
      chickens. ILTV can establish latency and reactivate later in life, but there have 
      been few investigations of ILTV latency. This study aimed to contribute to the 
      methodologies available to detect latent ILTV. A nested PCR was developed which 
      was more sensitive than three other molecular methods investigated in this study. 
      This nested PCR was then used in conjunction with in vitro reactivation culture 
      methods that were optimized and applied to trigeminal ganglia (TG) and tracheal 
      samples from ILTV-vaccinated commercial layer birds (n = 30). ILTV DNA was 
      detected by nested PCR in the upper respiratory tract (URT) or eye of 22 birds. 
      Of the remaining 8 birds, ILTV could be detected by co-culture in TG of 5 birds, 
      with reactivated virus mostly detected 6 days post-explant (dpe). ILTV was also 
      detected in tracheal cultures by 6 dpe. In the ILTV-positive URT samples, the 
      virus could be characterised as vaccine strains SA2 (n = 9) or A20 (n = 5). This 
      study provides evidence for reactivation and shedding of vaccine ILTV in 
      commercial layer birds. Moreover, this study produced a molecular and in-vitro 
      culture method to detect latent viral infection.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Thilakarathne, Dulari S
AU  - Thilakarathne DS
AD  - Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The 
      University of Melbourne, Parkville, Victoria, Australia. Electronic address: 
      dthilakarath@student.unimelb.edu.au.
FAU - Hartley, Carol A
AU  - Hartley CA
AD  - Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The 
      University of Melbourne, Parkville, Victoria, Australia.
FAU - Diaz-Mendez, Andres
AU  - Diaz-Mendez A
AD  - Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The 
      University of Melbourne, Parkville, Victoria, Australia.
FAU - Coppo, Mauricio J C
AU  - Coppo MJC
AD  - Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The 
      University of Melbourne, Parkville, Victoria, Australia.
FAU - Devlin, Joanne M
AU  - Devlin JM
AD  - Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, The 
      University of Melbourne, Parkville, Victoria, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191209
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/*methods
MH  - Chickens/virology
MH  - Herpesviridae Infections/*diagnosis/*veterinary
MH  - Herpesvirus 1, Gallid/genetics/growth & development/*isolation & purification
MH  - Latent Infection/*diagnosis/*veterinary/virology
MH  - Limit of Detection
MH  - Polymerase Chain Reaction
MH  - Poultry Diseases/diagnosis/virology
MH  - Trachea/virology
MH  - Viral Proteins/genetics
MH  - Viral Vaccines/analysis
OTO - NOTNLM
OT  - Culture methods
OT  - ILTV
OT  - Latency
OT  - Molecular detection
COIS- Declaration of Competing Interest No competing interests.
EDAT- 2019/12/11 06:00
MHDA- 2021/05/29 06:00
CRDT- 2019/12/11 06:00
PHST- 2019/08/19 00:00 [received]
PHST- 2019/12/03 00:00 [revised]
PHST- 2019/12/07 00:00 [accepted]
PHST- 2019/12/11 06:00 [pubmed]
PHST- 2021/05/29 06:00 [medline]
PHST- 2019/12/11 06:00 [entrez]
AID - S0166-0934(19)30380-5 [pii]
AID - 10.1016/j.jviromet.2019.113797 [doi]
PST - ppublish
SO  - J Virol Methods. 2020 Mar;277:113797. doi: 10.1016/j.jviromet.2019.113797. Epub 
      2019 Dec 9.