PMID- 31822637
OWN - NLM
STAT- MEDLINE
DCOM- 20201023
LR  - 20201023
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 11
IP  - 23
DP  - 2019 Dec 10
TI  - Nickel induces inflammatory activation via NF-kappaB, MAPKs, IRF3 and NLRP3 
      inflammasome signaling pathways in macrophages.
PG  - 11659-11672
LID - 10.18632/aging.102570 [doi]
AB  - Nickel (Ni), an environmental hazard, widely causes allergic contact 
      hypersensitivity worldwide. Despite that Ni-stimulated pro-inflammatory response 
      is vital in allergy, the underlying molecular mechanisms remain largely unclear. 
      Here, we demonstrated that NiCl(2) activated nuclear factor kappa B (NF-kappaB), 
      mitogen-activated protein kinases (MAPKs) and interferon regulatory factor 3 
      (IRF3) signaling pathways in primary bone marrow-derived macrophages (BMDMs), 
      leading to the altered transcription levels of interleukin-1beta (IL-1beta), -6, -8, 
      -18, tumor necrosis factor-alpha (TNF-alpha) and interferon beta (INF-beta). We also found that 
      nickel chloride (NiCl(2)) activated Nod-like receptor 3 (NLRP3) inflammasome 
      pathway, resulting in the proteolytic cleavage and release of IL-1beta. NiCl(2) 
      induced the accumulation of mitochondrial reactive oxygen species (mtROS) and the 
      release of mitochondrial DNA (mtDNA), thus activating NLRP3 inflammasome pathway. 
      Additionally, NiCl(2)-induced apoptosis was dependent on the generation of mtROS, 
      and caspase-1 activation might also partly contribute to the apoptotic process. 
      Altogether, abovementioned results indicate that NiCl(2) induces inflammatory 
      activation in BMDMs via NF-kappaB, MAPKs, IRF3 signaling pathways as well as NLRP3 
      inflammasome pathway, which provides a mechanism to improve the efficiency of 
      treatment against Ni-induced allergic reactions.
FAU - Guo, Hongrui
AU  - Guo H
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Liu, Huan
AU  - Liu H
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Jian, Zhijie
AU  - Jian Z
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Cui, Hengmin
AU  - Cui H
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
AD  - Key Laboratory of Agricultural Information Engineering of Sichuan Province, 
      Sichuan Agriculture University, Yaan, Sichuan 625014, China.
FAU - Fang, Jing
AU  - Fang J
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Zuo, Zhicai
AU  - Zuo Z
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Deng, Junliang
AU  - Deng J
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Li, Yinglun
AU  - Li Y
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Wang, Xun
AU  - Wang X
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Zhao, Ling
AU  - Zhao L
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
AD  - Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, 
      Sichuan Agriculture University, Wenjiang, Chengdu 611130, China.
FAU - Geng, Yi
AU  - Geng Y
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Ouyang, Ping
AU  - Ouyang P
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Lai, Weiming
AU  - Lai W
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Chen, Zhengli
AU  - Chen Z
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
FAU - Huang, Chao
AU  - Huang C
AD  - College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 
      Chengdu 611130, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191210
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Environmental Pollutants)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Irf3 protein, mouse)
RN  - 0 (NF-kappa B)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 7OV03QG267 (Nickel)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cell Survival/drug effects
MH  - Environmental Pollutants
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Inflammation/*chemically induced/metabolism
MH  - Interferon Regulatory Factor-3/genetics/*metabolism
MH  - Macrophages/*drug effects/metabolism
MH  - Membrane Potential, Mitochondrial
MH  - Mice
MH  - Mitochondria/drug effects/physiology
MH  - Mitogen-Activated Protein Kinase Kinases/genetics/*metabolism
MH  - NF-kappa B/*metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/genetics/metabolism
MH  - Nickel/*pharmacology
MH  - Reactive Oxygen Species
PMC - PMC6932914
OTO - NOTNLM
OT  - BMDMs
OT  - NF-kappaB
OT  - NiCl2
OT  - apoptosis
OT  - inflammasome
COIS- CONFLICTS OF INTEREST: The authors declare that there are no conflicts of 
      interest.
EDAT- 2019/12/12 06:00
MHDA- 2020/10/24 06:00
PMCR- 2019/12/15
CRDT- 2019/12/12 06:00
PHST- 2019/10/11 00:00 [received]
PHST- 2019/11/23 00:00 [accepted]
PHST- 2019/12/12 06:00 [pubmed]
PHST- 2020/10/24 06:00 [medline]
PHST- 2019/12/12 06:00 [entrez]
PHST- 2019/12/15 00:00 [pmc-release]
AID - 102570 [pii]
AID - 10.18632/aging.102570 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2019 Dec 10;11(23):11659-11672. doi: 10.18632/aging.102570. 
      Epub 2019 Dec 10.