PMID- 31824393 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1664-2295 (Print) IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 10 DP - 2019 TI - Normative Data and Minimally Detectable Change for Inner Retinal Layer Thicknesses Using a Semi-automated OCT Image Segmentation Pipeline. PG - 1117 LID - 10.3389/fneur.2019.01117 [doi] LID - 1117 AB - Neurodegenerative and neuroinflammatory diseases regularly cause optic nerve and retinal damage. Evaluating retinal changes using optical coherence tomography (OCT) in diseases like multiple sclerosis has thus become increasingly relevant. However, intraretinal segmentation, a necessary step for interpreting retinal changes in the context of these diseases, is not standardized and often requires manual correction. Here we present a semi-automatic intraretinal layer segmentation pipeline and establish normative values for retinal layer thicknesses at the macula, including dependencies on age, sex, and refractive error. Spectral domain OCT macular 3D volume scans were obtained from healthy participants using a Heidelberg Engineering Spectralis OCT. A semi-automated segmentation tool (SAMIRIX) based on an interchangeable third-party segmentation algorithm was developed and employed for segmentation, correction, and thickness computation of intraretinal layers. Normative data is reported from a 6 mm Early Treatment Diabetic Retinopathy Study (ETDRS) circle around the fovea. An interactive toolbox for the normative database allows surveying for additional normative data. We cross-sectionally evaluated data from 218 healthy volunteers (144 females/74 males, age 36.5 +/- 12.3 years, range 18-69 years). Average macular thickness (MT) was 313.70 +/- 12.02 mum, macular retinal nerve fiber layer thickness (mRNFL) 39.53 +/- 3.57 mum, ganglion cell and inner plexiform layer thickness (GCIPL) 70.81 +/- 4.87 mum, and inner nuclear layer thickness (INL) 35.93 +/- 2.34 mum. All retinal layer thicknesses decreased with age. MT and GCIPL were associated with sex, with males showing higher thicknesses. Layer thicknesses were also positively associated with each other. Repeated-measurement reliability for the manual correction of automatic intraretinal segmentation results was excellent, with an intra-class correlation coefficient >0.99 for all layers. The SAMIRIX toolbox can simplify intraretinal segmentation in research applications, and the normative data application may serve as an expandable reference for studies, in which normative data cannot be otherwise obtained. CI - Copyright (c) 2019 Motamedi, Gawlik, Ayadi, Zimmermann, Asseyer, Bereuter, Mikolajczak, Paul, Kadas and Brandt. FAU - Motamedi, Seyedamirhosein AU - Motamedi S AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Gawlik, Kay AU - Gawlik K AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Ayadi, Noah AU - Ayadi N AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Zimmermann, Hanna G AU - Zimmermann HG AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Asseyer, Susanna AU - Asseyer S AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Bereuter, Charlotte AU - Bereuter C AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Mikolajczak, Janine AU - Mikolajczak J AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Paul, Friedemann AU - Paul F AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Experimental and Clinical Research Center, Max Delbruck Center for Molecular Medicine and Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Department of Neurology, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Kadas, Ella Maria AU - Kadas EM AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. FAU - Brandt, Alexander Ulrich AU - Brandt AU AD - NeuroCure Clinical Research Center, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health, Berlin, Germany. AD - Department of Neurology, University of California, Irvine, Irvine, CA, United States. LA - eng PT - Journal Article DEP - 20191125 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 PMC - PMC6886563 OTO - NOTNLM OT - healthy population OT - inner retinal layer OT - macula OT - minimally detectable change OT - normative data OT - optical coherence tomography (OCT) OT - retina OT - segmentation EDAT- 2019/12/12 06:00 MHDA- 2019/12/12 06:01 PMCR- 2019/11/25 CRDT- 2019/12/12 06:00 PHST- 2019/05/08 00:00 [received] PHST- 2019/10/07 00:00 [accepted] PHST- 2019/12/12 06:00 [entrez] PHST- 2019/12/12 06:00 [pubmed] PHST- 2019/12/12 06:01 [medline] PHST- 2019/11/25 00:00 [pmc-release] AID - 10.3389/fneur.2019.01117 [doi] PST - epublish SO - Front Neurol. 2019 Nov 25;10:1117. doi: 10.3389/fneur.2019.01117. eCollection 2019.