PMID- 31825195
OWN - NLM
STAT- MEDLINE
DCOM- 20201211
LR  - 20240329
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Print)
IS  - 1944-8244 (Linking)
VI  - 12
IP  - 7
DP  - 2020 Feb 19
TI  - A New Strategy for Reporting Specific Protein Binding Events at Aqueous-Liquid 
      Crystal Interfaces in the Presence of Non-Specific Proteins.
PG  - 7869-7878
LID - 10.1021/acsami.9b16867 [doi]
AB  - Aqueous-liquid crystal (LC) interfaces offer promise as responsive interfaces at 
      which biomolecular recognition events can be amplified into macroscopic signals. 
      However, the design of LC interfaces that distinguish between specific and 
      non-specific protein interactions remains an unresolved challenge. Herein, we 
      report the synthesis of amphiphilic monomers, dimers, and trimers conjugated to 
      sulfonamide ligands via triazole rings, their assembly at aqueous-LC interfaces, 
      and the orientational response of LCs to the interactions of carbonic anhydrase 
      II (CAII) and serum albumin with the oligomer-decorated LC interfaces. Of six 
      oligomers synthesized, only dimers without amide methylation were found to 
      assemble at aqueous interfaces of nematic 4-cyano-4'-pentylbiphenyl (5CB) to 
      induce perpendicular LC orientations. At dimer-decorated LC interfaces, we found 
      that concentrations of CAII less than 4 muM did not measurably perturb the LC but 
      prevented non-specific adsorption and penetration of serum albumin into the 
      dimer-decorated interface that otherwise triggered bright, globular LC optical 
      domains. These experiments and others (including competitive adsorption of CAII, 
      BSA, and lysozyme) support our hypothesis that specific binding of CAII to the 
      dimer prevents LC anchoring transitions triggered by non-specific adsorption of 
      serum albumin. We illustrate the utility of the approach by reporting (i) the 
      relative activity of two small-molecule inhibitors 
      (6-ethoxy-2-benzothiazolesulfonamide and benzenesulfonamide) of CAII to 
      sulfonamide and (ii) proteolytic digestion of a protein (CAII) by thermolysin. 
      Overall, the results in this paper provide new insight into the interactions of 
      proteins at aqueous-LC interfaces and fresh ideas for either blocking 
      non-specific interactions of proteins at surfaces or reporting specific binding 
      events at LC interfaces in the presence of non-specific proteins.
FAU - Park, Chul Soon
AU  - Park CS
AD  - Department of Chemical and Biological Engineering , University of 
      Wisconsin-Madison , Madison , Wisconsin 53706 , United States.
FAU - Iwabata, Kazuki
AU  - Iwabata K
AD  - Department of Chemical and Biological Engineering , University of 
      Wisconsin-Madison , Madison , Wisconsin 53706 , United States.
FAU - Sridhar, Uma
AU  - Sridhar U
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
FAU - Tsuei, Michael
AU  - Tsuei M
AD  - Smith School of Chemical and Biomolecular Engineering , Cornell University , 
      Ithaca , New York 14853 , United States.
FAU - Singh, Khushboo
AU  - Singh K
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
FAU - Kim, Young-Ki
AU  - Kim YK
AUID- ORCID: 0000-0002-4420-3489
AD  - Smith School of Chemical and Biomolecular Engineering , Cornell University , 
      Ithaca , New York 14853 , United States.
AD  - Department of Chemical Engineering , Pohang University of Science and Technology 
      , Pohang , Gyeongbuk 37673 , Korea.
FAU - Thayumanavan, S
AU  - Thayumanavan S
AUID- ORCID: 0000-0002-6475-6726
AD  - Department of Chemistry , University of Massachusetts Amherst , Amherst , 
      Massachusetts 01003 , United States.
FAU - Abbott, Nicholas L
AU  - Abbott NL
AUID- ORCID: 0000-0002-9653-0326
AD  - Smith School of Chemical and Biomolecular Engineering , Cornell University , 
      Ithaca , New York 14853 , United States.
LA  - eng
GR  - R01 GM065255/GM/NIGMS NIH HHS/United States
GR  - T32 GM108556/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20200207
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Biphenyl Compounds)
RN  - 0 (Ethyl Ethers)
RN  - 0 (Ligands)
RN  - 0 (Nitriles)
RN  - 0 (Polymers)
RN  - 0 (Proteins)
RN  - 0 (Serum Albumin)
RN  - 0 (Sulfonamides)
RN  - 0 (Triazoles)
RN  - 059QF0KO0R (Water)
RN  - 40817-08-1 (4-cyano-4'-pentylbiphenyl)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.4.24.27 (Thermolysin)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Adsorption
MH  - Biphenyl Compounds/*chemistry
MH  - Carbonic Anhydrase II/chemistry
MH  - Ethyl Ethers/chemistry
MH  - Ligands
MH  - Liquid Crystals/*chemistry
MH  - Microscopy
MH  - Molecular Structure
MH  - Muramidase/chemistry
MH  - Nitriles/*chemistry
MH  - Polymers/*chemistry
MH  - Protein Binding
MH  - Proteins/*chemistry
MH  - Serum Albumin/chemistry
MH  - Sulfonamides/*chemistry
MH  - Surface Properties
MH  - Thermolysin/chemistry
MH  - Triazoles/chemistry
MH  - Water/*chemistry
MH  - Benzenesulfonamides
PMC - PMC7368459
MID - NIHMS1606139
OTO - NOTNLM
OT  - inhibitors
OT  - liquid crystal
OT  - non-specific
OT  - oligomers
OT  - proteins
OT  - specific binding
COIS- CONFLICT OF INTEREST Nicholas Abbott has a financial interest in Platypus 
      Technologies LLC, a company that has commercialized liquid crystal-based 
      analytical technology The authors declare no competing financial interest
EDAT- 2019/12/12 06:00
MHDA- 2020/12/15 06:00
PMCR- 2021/02/19
CRDT- 2019/12/12 06:00
PHST- 2019/12/12 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2019/12/12 06:00 [entrez]
PHST- 2021/02/19 00:00 [pmc-release]
AID - 10.1021/acsami.9b16867 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2020 Feb 19;12(7):7869-7878. doi: 
      10.1021/acsami.9b16867. Epub 2020 Feb 7.