PMID- 31827524
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2019
DP  - 2019
TI  - Shear Stress Promotes Arterial Endothelium-Oriented Differentiation of 
      Mouse-Induced Pluripotent Stem Cells.
PG  - 1847098
LID - 10.1155/2019/1847098 [doi]
LID - 1847098
AB  - Establishment of a functional vascular network, which is required in tissue 
      repair and regeneration, needs large-scale production of specific arterial or 
      venous endothelial cells (ECs) from stem cells. Previous in vitro studies by us 
      and others revealed that shear stress induces EC differentiation of bone 
      marrow-derived mesenchymal stem cells and embryonic stem cells. In this study, we 
      focused on the impact of different magnitudes of shear stress on the 
      differentiation of mouse-induced pluripotent stem cells (iPSCs) towards arterial 
      or venous ECs. When iPSCs were exposed to shear stress (5, 10, and 15 dyne/cm(2)) 
      with 50 ng/mL vascular endothelial growth factor and 10 ng/mL fibroblast growth 
      factor, the expression levels of the general EC markers and the arterial markers 
      increased, and the stress amplitude of 10 dyne/cm(2) could be regarded as a 
      proper promoter, whereas the venous and lymphatic markers had little or no 
      expression. Further, shear stress caused cells to align parallel to the direction 
      of the flow, induced cells forming functional tubes, and increased the secretion 
      of nitric oxide. In addition, Notch1 was significantly upregulated, and the Notch 
      ligand Delta-like 4 was activated in response to shear stress, while inhibition 
      of Notch signaling by DAPT remarkably abolished the shear stress-induced arterial 
      epithelium differentiation. Taken together, our results indicate that exposure to 
      appropriate shear stress facilitated the differentiation of mouse iPSCs towards 
      arterial ECs via Notch signaling pathways, which have potential applications for 
      both disease modeling and regenerative medicine.
CI  - Copyright (c) 2019 Yan Huang et al.
FAU - Huang, Yan
AU  - Huang Y
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
AD  - Beijing Advanced Innovation Center for Biomedical Engineering, Beihang 
      University, Beijing 100083, China.
FAU - Chen, Xiaofang
AU  - Chen X
AUID- ORCID: 0000-0003-2058-5731
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
AD  - Beijing Advanced Innovation Center for Biomedical Engineering, Beihang 
      University, Beijing 100083, China.
FAU - Che, Jifei
AU  - Che J
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
FAU - Zhan, Qi
AU  - Zhan Q
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
FAU - Ji, Jing
AU  - Ji J
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
AD  - Beijing Advanced Innovation Center for Biomedical Engineering, Beihang 
      University, Beijing 100083, China.
FAU - Fan, Yubo
AU  - Fan Y
AUID- ORCID: 0000-0002-3480-4395
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, China.
AD  - Beijing Advanced Innovation Center for Biomedical Engineering, Beihang 
      University, Beijing 100083, China.
AD  - Beijing Key Laboratory of Rehabilitation Technical Aids for Old-Age Disability, 
      National Research Center for Rehabilitation Technical Aids, Beijing 100176, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20191115
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC6881757
COIS- The authors declare to have no conflicts of interests.
EDAT- 2019/12/13 06:00
MHDA- 2019/12/13 06:01
PMCR- 2019/11/15
CRDT- 2019/12/13 06:00
PHST- 2019/01/31 00:00 [received]
PHST- 2019/09/05 00:00 [revised]
PHST- 2019/10/17 00:00 [accepted]
PHST- 2019/12/13 06:00 [entrez]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2019/12/13 06:01 [medline]
PHST- 2019/11/15 00:00 [pmc-release]
AID - 10.1155/2019/1847098 [doi]
PST - epublish
SO  - Stem Cells Int. 2019 Nov 15;2019:1847098. doi: 10.1155/2019/1847098. eCollection 
      2019.