PMID- 31827695
OWN - NLM
STAT- MEDLINE
DCOM- 20200504
LR  - 20200505
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2019
DP  - 2019
TI  - CD47 Deficiency Attenuates Isoproterenol-Induced Cardiac Remodeling in Mice.
PG  - 7121763
LID - 10.1155/2019/7121763 [doi]
LID - 7121763
AB  - In this study, we investigated whether CD47 deficiency attenuates isoproterenol- 
      (ISO-) induced cardiac remodeling in mice. Cardiac remodeling was induced by 
      intraperitoneal (i.p.) injection of ISO (60 mg.kg(-1).d(-1) in 100 mul of sterile 
      normal saline) daily for 14 days and was confirmed by increased levels of lactate 
      dehydrogenase (LDH) and creatine kinase MB (CK-MB), increased heart weight to 
      body weight (HW/BW) ratios, and visible cardiac fibrosis. Apoptosis was evaluated 
      by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      staining. Levels of malondialdehyde (MDA) and reactive oxygen species (ROS) were 
      found to be significantly higher in the ISO group than in the control group, 
      while superoxide dismutase (SOD) levels were suppressed in the ISO group. 
      However, CD47 knockout significantly limited ISO-induced increases in LDH, CK-MB, 
      and HW/BW ratios, cardiac fibrosis, oxidative stress, and apoptosis in the heart. 
      In addition, CD47 deficiency also increased p-AMPK and LAMP2 expression and 
      decreased HDAC3, cleaved Caspase-3, cleaved Caspase-9, LC3II, and p62 expression 
      in cardiac tissues. In conclusion, CD47 deficiency reduced i.p. ISO-induced 
      cardiac remodeling probably by inhibiting the HDAC3 pathway, improving AMPK 
      signaling and autophagy flux, and rescuing autophagic clearance.
CI  - Copyright (c) 2019 Zhi Zuo et al.
FAU - Zuo, Zhi
AU  - Zuo Z
AUID- ORCID: 0000-0001-8827-3676
AD  - Department of Cardiovascular, Zhongda Hospital Affiliated to Southeast 
      University, No. 87 Dingjiaqiao, Nanjing, Jiangsu, China.
FAU - Ji, Ming-Yue
AU  - Ji MY
AD  - Department of Cardiovascular, Lianshui People's Hospital, No. 6, East Hongri 
      Avenue, Huaian, Jiangsu, China.
FAU - Zhao, Kun
AU  - Zhao K
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing, Jiangsu, China.
FAU - Su, Zhong-Ping
AU  - Su ZP
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing, Jiangsu, China.
FAU - Li, Peng
AU  - Li P
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing, Jiangsu, China.
FAU - Hou, Dao-Rong
AU  - Hou DR
AUID- ORCID: 0000-0002-7755-9326
AD  - Key Laboratory of Model Animal Research, Animal Core Facility of Nanjing Medical 
      University, Nanjing Medical University, 101 Longmian Avenue, Nanjing, Jiangsu, 
      China.
FAU - Li, Yong
AU  - Li Y
AUID- ORCID: 0000-0002-9619-1694
AD  - Department of Cardiology, The First Affiliated Hospital of Nanjing Medical 
      University, 300 Guangzhou Road, Nanjing, Jiangsu, China.
LA  - eng
PT  - Journal Article
DEP - 20191119
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (CD47 Antigen)
RN  - 0 (Cardiotonic Agents)
RN  - 0 (Cd47 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - L628TT009W (Isoproterenol)
SB  - IM
MH  - Animals
MH  - CD47 Antigen/*physiology
MH  - Cardiomegaly/chemically induced/pathology/*prevention & control
MH  - Cardiotonic Agents/*toxicity
MH  - Isoproterenol/*toxicity
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oxidative Stress/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Ventricular Remodeling/drug effects/*physiology
PMC - PMC6885801
COIS- All authors declare that they have no competing interests.
EDAT- 2019/12/13 06:00
MHDA- 2020/05/06 06:00
PMCR- 2019/11/19
CRDT- 2019/12/13 06:00
PHST- 2019/03/17 00:00 [received]
PHST- 2019/09/11 00:00 [accepted]
PHST- 2019/12/13 06:00 [entrez]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
PHST- 2019/11/19 00:00 [pmc-release]
AID - 10.1155/2019/7121763 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2019 Nov 19;2019:7121763. doi: 10.1155/2019/7121763. 
      eCollection 2019.