PMID- 31827697
OWN - NLM
STAT- MEDLINE
DCOM- 20200504
LR  - 20200505
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2019
DP  - 2019
TI  - Overexpression of Inducible Nitric Oxide Synthase in Allergic and Nonallergic 
      Nasal Polyp.
PG  - 7506103
LID - 10.1155/2019/7506103 [doi]
LID - 7506103
AB  - Sinonasal polyps are very common benign lesions of the nasal mucosa. Most of 
      nasal polyps (NP) are idiopathic, and the pathophysiology of this disease is 
      still incompletely understood. Nitric oxide (NO) is a reactive molecule generated 
      by nitric oxide synthase (NOS). NO has been identified as an important mediator 
      in airway function and pathogenesis of several respiratory system diseases. 
      Histological and genetical expression of iNOS was detected to evaluate the role 
      of NO in the pathogenesis of allergic (ANP) and nonallergic nasal polyps (NANP). 
      Forty patients with nasal polyps (20 allergic and 20 nonallergic) were identified 
      by history, clinical examination, and investigation. NPs were obtained from the 
      middle turbinate (MT) during concha bullosa surgery. Twenty normal MT nasal 
      tissues were taken as the control from patients undergoing concha bullosa 
      surgery, without any evidence of allergy or inflammation. A nasal polyp specimen 
      from each patient was subjected for immune-histochemical study followed by 
      histological examination to detect the expression of iNOS. RT-PCR was used to 
      evaluate the iNOS gene expression in isolated tissues. The expression of iNOS in 
      both epithelial and stromal layers was greater in NP than in MT tissues. The ANP 
      group showed more iNOS expression than those of the NANP group. The relative mRNA 
      levels of iNOS gene were significantly higher in ANP (2.5-fold) compared to the 
      normal (1.02-fold, P < 0.001) and NANP (1.5-fold, P < 0.01) groups. NP exhibited 
      a significantly high expression of iNOS at both histological and genetical 
      levels. NO might be an essential factor in the life history of NP. Further 
      studies in a larger sample size are required to explain the probable mechanisms 
      of NO in pathogenesis of NP.
CI  - Copyright (c) 2019 Ahmed Adel Sadek et al.
FAU - Sadek, Ahmed Adel
AU  - Sadek AA
AD  - Department of Otorhinolaryngology, Faculty of Medicine, Minia University, Minia 
      61511, Egypt.
FAU - Abdelwahab, Soha
AU  - Abdelwahab S
AD  - Department of Histology, Faculty of Medicine, Minia University, Minia 61511, 
      Egypt.
FAU - Eid, Safaa Yehia
AU  - Eid SY
AD  - Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Saudi Arabia.
FAU - Almaimani, Riyad A
AU  - Almaimani RA
AD  - Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Saudi Arabia.
FAU - Althubiti, Mohammad A
AU  - Althubiti MA
AUID- ORCID: 0000-0002-9738-3188
AD  - Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Saudi Arabia.
FAU - El-Readi, Mahmoud Zaki
AU  - El-Readi MZ
AUID- ORCID: 0000-0001-8398-5412
AD  - Department of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah, 
      Saudi Arabia.
AD  - Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University, 71524 
      Assiut, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20191107
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Biomarkers)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers/*metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Hypersensitivity/*diagnosis/genetics/metabolism
MH  - Inflammation/*diagnosis/genetics/metabolism
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/*diagnosis/genetics/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type II/genetics/*metabolism
MH  - Prospective Studies
MH  - Young Adult
PMC - PMC6885221
COIS- The authors stated that there is no conflict of interest.
EDAT- 2019/12/13 06:00
MHDA- 2020/05/06 06:00
PMCR- 2019/11/07
CRDT- 2019/12/13 06:00
PHST- 2019/01/10 00:00 [received]
PHST- 2019/05/15 00:00 [revised]
PHST- 2019/05/27 00:00 [accepted]
PHST- 2019/12/13 06:00 [entrez]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
PHST- 2019/11/07 00:00 [pmc-release]
AID - 10.1155/2019/7506103 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2019 Nov 7;2019:7506103. doi: 10.1155/2019/7506103. 
      eCollection 2019.