PMID- 31828107 OWN - NLM STAT- MEDLINE DCOM- 20200427 LR - 20220411 IS - 2314-6141 (Electronic) IS - 2314-6133 (Print) VI - 2019 DP - 2019 TI - Cell-to-Cell Culture Inhibits Dedifferentiation of Chondrocytes and Induces Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells. PG - 5871698 LID - 10.1155/2019/5871698 [doi] LID - 5871698 AB - BACKGROUND: Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) possess great promise as a therapeutic to repair damaged cartilage. Direct intra-articular injection of mesenchymal stem cells has been shown to reduce cartilage damage and is advantageous as surgical implantation and associated side effects can be avoided using this approach. However, the efficacy of stem cell-based therapy for cartilage repair depends highly on the direct interactions of these stem cells with chondrocytes in the joint. In this study, we have carried out an in vitro cell-to-cell contact coculture study with human articular chondrocytes (hACs) and hUC-MSCs, with the goal of this study being to evaluate interactions between hACs and hUC-MSCs. METHODS: Low-density monolayer cultures of hUC-MSCs and hACs were mixed at a ratio of 1 : 1 in direct cell-to-cell contact groups. Results were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blot, enzyme-linked immunosorbent assay (ELISA), and immunofluorescence. RESULTS: A mixed coculture of hUC-MSCs and hACs was found to exhibit synergistic interactions with enhanced differentiation of hUC-MSCs and reduced dedifferentiation of chondrocytes. Mixed cultures after 21 days were found to exhibit sufficient chondrogenic induction. CONCLUSIONS: The results from this study suggest the presence of mutual effects between hUC-MSCs and hACs even culture at low density and provide further support for the use of intra-articular injection strategies for cartilage defect treatment. CI - Copyright (c) 2019 Xingfu Li et al. FAU - Li, Xingfu AU - Li X AD - Guangzhou Medical University, Guangzhou, Guangdong Province, China. AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. FAU - Liang, Yujie AU - Liang Y AD - Department of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China. FAU - Xu, Xiao AU - Xu X AD - Guangzhou Medical University, Guangzhou, Guangdong Province, China. AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. FAU - Xiong, Jianyi AU - Xiong J AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. FAU - Ouyang, Kan AU - Ouyang K AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. FAU - Duan, Li AU - Duan L AUID- ORCID: 0000-0002-3638-8102 AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. FAU - Wang, Daping AU - Wang D AUID- ORCID: 0000-0002-2402-8134 AD - Guangzhou Medical University, Guangzhou, Guangdong Province, China. AD - Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopedic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Affiliated Hospital of Shenzhen University, Health Science Center), Shenzhen 518035, Guangdong Province, China. LA - eng PT - Journal Article DEP - 20191116 PL - United States TA - Biomed Res Int JT - BioMed research international JID - 101600173 RN - 9007-34-5 (Collagen) SB - IM MH - Adult MH - Cell Differentiation/*physiology MH - Cells, Cultured MH - Chondrocytes/*cytology/metabolism MH - Coculture Techniques/*methods MH - Collagen/analysis/genetics/metabolism MH - Human Umbilical Vein Endothelial Cells/*cytology/metabolism MH - Humans MH - Mesenchymal Stem Cells/*cytology/metabolism PMC - PMC6885164 COIS- The authors declare that they have no conflicts of interest. EDAT- 2019/12/13 06:00 MHDA- 2020/04/28 06:00 PMCR- 2019/11/16 CRDT- 2019/12/13 06:00 PHST- 2019/07/14 00:00 [received] PHST- 2019/09/13 00:00 [revised] PHST- 2019/10/08 00:00 [accepted] PHST- 2019/12/13 06:00 [entrez] PHST- 2019/12/13 06:00 [pubmed] PHST- 2020/04/28 06:00 [medline] PHST- 2019/11/16 00:00 [pmc-release] AID - 10.1155/2019/5871698 [doi] PST - epublish SO - Biomed Res Int. 2019 Nov 16;2019:5871698. doi: 10.1155/2019/5871698. eCollection 2019.