PMID- 31828743
OWN - NLM
STAT- MEDLINE
DCOM- 20200113
LR  - 20201220
IS  - 1833-3516 (Print)
IS  - 2209-1491 (Electronic)
IS  - 1833-3516 (Linking)
VI  - 49
IP  - 4
DP  - 2019 Dec 20
TI  - Protective effect of hyperbaric oxygen treatment on rat intestinal mucosa after 
      mesenteric ischaemia and reperfusion.
PG  - 253-258
LID - 10.28920/dhm49.4.253-258 [doi]
AB  - INTRODUCTION: Mesenteric ischaemia results from a lack of adequate blood flow to 
      and oxygenation of the mesentery and intestines. The aim of the present study was 
      to evaluate the effect of hyperbaric oxygen treatment (HBOT) on the healing 
      process in intestinal mucosa of rats undergoing mesenteric ischaemia and 
      reperfusion. METHODS: Thirty-two Wistar-Albino rats were divided into four groups 
      of eight: 1) ischaemia/reperfusion (I/R); 2) sham operation; 3) I/R+HBOT started 
      6 hours after reperfusion; 4) I/R+HBOT started 12 hours after reperfusion. In the 
      I/R groups, a vascular clamp was placed across the superior mesenteric artery to 
      occlude arterial circulation for 60 minutes, followed by reperfusion. A dose of 
      HBOT consisted of 100% oxygen breathing for 90 minutes at 2.5 atmospheres 
      absolute pressure. Thirteen doses of HBOT were administered after ischaemia. The 
      rats were sacrificed on the eighth day, and their intestinal tissues were 
      harvested for histopathologic analysis. The tissue levels of catalase, 
      malondialdehyde, and glutathione were determined. RESULTS: The histopathological 
      scores (HSCORE) were consistent with macroscopic examinations. The scores were 
      significantly higher (worse) in Group 1 compared to Group 2, Group 3, and Group 4 
      (for all comparisons, P < 0.05). Group 4's HSCORE was significantly higher than 
      those of Group 2 and Group 3 (for both comparisons P < 0.05). Group 3's HSCOREs 
      were only marginally higher than Group 2. Group 3 exhibited higher glutathione 
      levels than Group 1 (P < 0.05). There were no significant differences across the 
      groups with respect to malondialdehyde and catalase levels. CONCLUSION: A 
      beneficial effect of HBOT was observed on oxidative stress and inflammation in 
      acute mesenteric ischaemia-reperfusion.
CI  - Copyright: This article is the copyright of the authors who grant Diving and 
      Hyperbaric Medicine a non-exclusive licence to publish the article in electronic 
      and other forms.
FAU - Aciksari, Kurtulus
AU  - Aciksari K
AD  - Department of Emergency Medicine, Faculty of Medicine, Istanbul Medeniyet 
      University, Istanbul, Turkey.
AD  - Corresponding author: Department of Emergency Medicine, Faculty of Medicine, 
      Istanbul Medeniyet University, Istanbul, Turkey, 
      kurtulus.aciksari@medeniyet.edu.tr.
FAU - Egin, Seracettin
AU  - Egin S
AD  - Department of General Surgery, MoH Okmeydani Training and Research Hospital, 
      Istanbul, Turkey.
FAU - Hepgul, Gulcin
AU  - Hepgul G
AD  - Department of General Surgery, Bagcilar Training and Research Hospital, Istanbul, 
      Turkey.
FAU - Mirasoglu, Bengusu
AU  - Mirasoglu B
AD  - Department of Underwater and Hyperbaric Medicine, Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
FAU - Tanriverdi, Gamze
AU  - Tanriverdi G
AD  - Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
FAU - Kanber, Devrim S
AU  - Kanber DS
AD  - Department of Biophysics, Cerrahpasa Faculty of Medicine, Istanbul University, 
      Istanbul, Turkey.
FAU - Demirci, Sibel
AU  - Demirci S
AD  - Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
FAU - Dogan, Halil
AU  - Dogan H
AD  - Department of Emergency Medicine, MoH Bakirkoy Dr. Sadi Konuk Training and 
      Research Hospital, Istanbul, Turkey.
FAU - Ozucelik, Dogac N
AU  - Ozucelik DN
AD  - Health Sciences Faculty, Istanbul University, Istanbul, Turkey.
FAU - Toklu, Akin S
AU  - Toklu AS
AD  - Department of Underwater and Hyperbaric Medicine, Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
FAU - Seckin, Ismail
AU  - Seckin I
AD  - Department of Histology and Embryology, Cerrahpasa Faculty of Medicine, Istanbul 
      University, Istanbul, Turkey.
FAU - Yanar, Hakan T
AU  - Yanar HT
AD  - Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, 
      Istanbul, Turkey.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Diving Hyperb Med
JT  - Diving and hyperbaric medicine
JID - 101282742
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - *Hyperbaric Oxygenation/methods
MH  - Intestinal Mucosa/pathology
MH  - *Mesenteric Ischemia/prevention & control
MH  - Oxygen
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - *Reperfusion Injury/prevention & control
PMC - PMC7039772
OTO - NOTNLM
OT  - Experimental study
OT  - Histology
OT  - Ischaemia-reperfusion injury
OT  - Mesenteric ischaemia
EDAT- 2019/12/13 06:00
MHDA- 2020/01/14 06:00
PMCR- 2020/12/20
CRDT- 2019/12/13 06:00
PHST- 2019/04/06 00:00 [received]
PHST- 2019/07/25 00:00 [accepted]
PHST- 2019/12/13 06:00 [entrez]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2020/01/14 06:00 [medline]
PHST- 2020/12/20 00:00 [pmc-release]
AID - 10.28920/dhm49.4.253-258 [doi]
PST - ppublish
SO  - Diving Hyperb Med. 2019 Dec 20;49(4):253-258. doi: 10.28920/dhm49.4.253-258.