PMID- 31830656
OWN - NLM
STAT- MEDLINE
DCOM- 20200205
LR  - 20200205
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 704
DP  - 2020 Feb 20
TI  - Biodegradation of polyethylene microplastic particles by the fungus Aspergillus 
      flavus from the guts of wax moth Galleria mellonella.
PG  - 135931
LID - S0048-9697(19)35926-1 [pii]
LID - 10.1016/j.scitotenv.2019.135931 [doi]
AB  - Polyethylene (PE) products are widely used in daily life, agriculture, and 
      industry because of their convenience and economic value. However, PE is one of 
      the polymer materials remarkably resistant to degradation. Current methods of 
      plastic waste disposal pose a threat to the environment and produce microplastic 
      particles (MPP), which becomes a global environmental concern because of its 
      accumulation. In this study, a PE-degrading fungus Aspergillus flavus named 
      PEDX3, was isolated from the gut contents of wax moth Galleria mellonella. The 
      results indicated that high-density polyethylene (HDPE) MPP was degraded into the 
      MPP with a lower molecular weight by strain PEDX3 after 28 days incubation. In 
      addition, Fourier Transform - Infrared Spectroscopy (FT-IR) results showed the 
      appearance of carbonyl groups and ether groups of MPP, which also validated the 
      degradation of PE. Furthermore, the potential degradation enzymes were 
      investigated by Reverse Transcription-Polymerase Chain Reaction (RT-PCR). 
      Finally, two laccase-like multicopper oxidases (LMCOs) genes, AFLA_006190 and 
      AFLA_053930, displayed up-regulated expression during the degradation process, 
      which may be the candidate PE-degrading enzymes. These results have demonstrated 
      that the A. flavus strain PEDX3 has an ability to degrade microplastic particles 
      and the two PE-degrading enzymes provide a promising application for the PE MPP 
      remediation.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Zhang, Junqing
AU  - Zhang J
AD  - Department of Resources Science of Traditional Chinese Medicines and State Key 
      Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China 
      Pharmaceutical University, Nanjing, Jiangsu, PR China; School of Pharmacy, China 
      Pharmaceutical University, Nanjing, Jiangsu, PR China.
FAU - Gao, Danling
AU  - Gao D
AD  - School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, 
      Jiangsu, PR China.
FAU - Li, Quanhao
AU  - Li Q
AD  - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, PR China.
FAU - Zhao, Yixuan
AU  - Zhao Y
AD  - School of Life Science and Technology, China Pharmaceutical University, Nanjing, 
      Jiangsu, PR China.
FAU - Li, Li
AU  - Li L
AD  - School of Life Science and Technology, China Pharmaceutical University, Nanjing, 
      Jiangsu, PR China.
FAU - Lin, Hanfeng
AU  - Lin H
AD  - School of Life Science and Technology, China Pharmaceutical University, Nanjing, 
      Jiangsu, PR China.
FAU - Bi, Qirui
AU  - Bi Q
AD  - Department of TCMs Pharmaceuticals, School of Traditional Chinese Pharmacy, China 
      Pharmaceutical University, Nanjing 211198, PR China. Electronic address: 
      biqirui@cpu.edu.cn.
FAU - Zhao, Yucheng
AU  - Zhao Y
AD  - Department of Resources Science of Traditional Chinese Medicines and State Key 
      Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China 
      Pharmaceutical University, Nanjing, Jiangsu, PR China. Electronic address: 
      zhaoyucheng1986@126.com.
LA  - eng
PT  - Journal Article
DEP - 20191205
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Environmental Pollutants)
RN  - 0 (Microplastics)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Aspergillus flavus/*metabolism
MH  - Biodegradation, Environmental
MH  - Environmental Pollutants/*metabolism
MH  - Gastrointestinal Contents
MH  - Microplastics/*metabolism
MH  - Moths/*microbiology
MH  - Polyethylene/*metabolism
OTO - NOTNLM
OT  - Aspergillus flavus
OT  - Biodegradation
OT  - Galleria mellonella
OT  - Microplastic particles
OT  - Polyethylene degrading enzyme
EDAT- 2019/12/13 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/12/13 06:00
PHST- 2019/09/26 00:00 [received]
PHST- 2019/11/20 00:00 [revised]
PHST- 2019/12/02 00:00 [accepted]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/12/13 06:00 [entrez]
AID - S0048-9697(19)35926-1 [pii]
AID - 10.1016/j.scitotenv.2019.135931 [doi]
PST - ppublish
SO  - Sci Total Environ. 2020 Feb 20;704:135931. doi: 10.1016/j.scitotenv.2019.135931. 
      Epub 2019 Dec 5.