PMID- 31830667
OWN - NLM
STAT- MEDLINE
DCOM- 20200604
LR  - 20200604
IS  - 1878-4291 (Electronic)
IS  - 0968-4328 (Linking)
VI  - 129
DP  - 2020 Feb
TI  - Lippia sidoides and Lippia origanoides essential oils affect the viability, 
      motility and ultrastructure of Trypanosoma cruzi.
PG  - 102781
LID - S0968-4328(19)30264-1 [pii]
LID - 10.1016/j.micron.2019.102781 [doi]
AB  - Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered a public 
      health problem. The current chemotherapy for this illness causes serious side 
      effects and its use in the chronic phase of the disease is still controversial. 
      In this regard, the investigation of novel therapeutic strategies remains a 
      priority. The essential oils (EOs) from aromatic plants emerge as a promising 
      source of bioactive compounds. In a previous work we reported the trypanocidal 
      activity of the essential oils from the medicinal plants Lippia sidoides (LSEO) 
      and Lippia origanoides (LOEO) against T. cruzi. Herein, we aimed to further 
      investigate, in more details, the mode of action of LSEO and LOEO on the 
      different developmental stages of this parasite. We showed that Lippia sidoides 
      (LSEO) and Lippia origanoides (LOEO) induced a significant reduction in the 
      percentage of macrophages infected by T. cruzi and in the number of intracellular 
      parasites. Ultrastructural analysis showed that the treatment with both oils 
      caused morphological changes consistent with loss of viability and cell death. 
      The reduced staining with calcein and the increase in the proportion of 
      HE-positive cells also demonstrated that LSEO and LOEO caused loss of parasite 
      viability and membrane integrity. A considerable decrease in Rhodamine 123 and an 
      increase in fluorescence intensity of MitoSox in LOEO were indicative of loss of 
      mitochondrial potential and generation of reactive oxygen species, which 
      ultimately lead to parasite death. Moreover, the optical tweezer analysis 
      indicated that LOEO was more effective in reducing the motility of the 
      epimastigotes. Taken together, our results demonstrated that the LSEO and LOEO 
      are active against T. cruzi and constitute a promising drugs for the therapy of 
      Chagas disease.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - de Melo, Andrezza Raposo Borges
AU  - de Melo ARB
AD  - Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhaes, FIOCRUZ, PE, 
      Av. Prof. Moraes Rego s/n Cidade Universitaria-Campus da UFPE, Recife, PE, 
      50670-420, Brazil.
FAU - Maciel Higino, Taciana Mirely
AU  - Maciel Higino TM
AD  - Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhaes, FIOCRUZ, PE, 
      Av. Prof. Moraes Rego s/n Cidade Universitaria-Campus da UFPE, Recife, PE, 
      50670-420, Brazil.
FAU - da Rocha Oliveira, Aline Dulce Pitt
AU  - da Rocha Oliveira ADP
AD  - Departamento de Biofisica e Radiobiologia, Centro de Biociencias, Universidade 
      Federal de Pernambuco, Av. Professor Moraes Rego s/n Cidade Universitaria, 
      50670-901, Recife, PE, Brazil.
FAU - Fontes, Adriana
AU  - Fontes A
AD  - Departamento de Biofisica e Radiobiologia, Centro de Biociencias, Universidade 
      Federal de Pernambuco, Av. Professor Moraes Rego s/n Cidade Universitaria, 
      50670-901, Recife, PE, Brazil.
FAU - da Silva, Diego Cesar Nunes
AU  - da Silva DCN
AD  - Colegiado de Ciencias Biologicas, UNIVASF, Av. Jose de Sa Manicoba, S/N CEP 
      56304-917, Petrolina, PE, Brazil.
FAU - de Castro, Maria Carolina Accioly Brelaz
AU  - de Castro MCAB
AD  - Nucleo de Enfermagem, Centro Academico de Vitoria, Universidade Federal de 
      Pernambuco, Vitoria de Santo Antao, Brazil.
FAU - Dantas Lopes, Jose Arimateia
AU  - Dantas Lopes JA
AD  - Departamento de Quimica, Universidade Federal do Piaui, Campus Universitario 
      Ministro Petronio Portela-Ininga, Teresina, PI, 64049-550, Brazil.
FAU - de Figueiredo, Regina Celia Bressan Queiroz
AU  - de Figueiredo RCBQ
AD  - Departamento de Microbiologia, Centro de Pesquisa Aggeu Magalhaes, FIOCRUZ, PE, 
      Av. Prof. Moraes Rego s/n Cidade Universitaria-Campus da UFPE, Recife, PE, 
      50670-420, Brazil. Electronic address: bressan@cpqam.fiocruz.br.
LA  - eng
PT  - Journal Article
DEP - 20191112
PL  - England
TA  - Micron
JT  - Micron (Oxford, England : 1993)
JID - 9312850
RN  - 0 (Oils, Volatile)
RN  - 0 (Plant Oils)
RN  - 0 (Trypanocidal Agents)
SB  - IM
MH  - Cell Survival/drug effects
MH  - Chagas Disease/*drug therapy/parasitology/prevention & control
MH  - Lippia/chemistry
MH  - Oils, Volatile/*pharmacology
MH  - Plant Oils/*pharmacology
MH  - Trypanocidal Agents/*pharmacology
MH  - Trypanosoma cruzi/*drug effects/ultrastructure
OTO - NOTNLM
OT  - Chemotherapy
OT  - Essential oils
OT  - Lippia origanoides
OT  - Lippia sidoides
OT  - Optical tweezer
OT  - Trypanosoma cruzi
OT  - Ultrastructure
EDAT- 2019/12/13 06:00
MHDA- 2020/06/05 06:00
CRDT- 2019/12/13 06:00
PHST- 2019/08/15 00:00 [received]
PHST- 2019/11/11 00:00 [revised]
PHST- 2019/11/12 00:00 [accepted]
PHST- 2019/12/13 06:00 [pubmed]
PHST- 2020/06/05 06:00 [medline]
PHST- 2019/12/13 06:00 [entrez]
AID - S0968-4328(19)30264-1 [pii]
AID - 10.1016/j.micron.2019.102781 [doi]
PST - ppublish
SO  - Micron. 2020 Feb;129:102781. doi: 10.1016/j.micron.2019.102781. Epub 2019 Nov 12.