PMID- 31830839
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1473-2300 (Electronic)
IS  - 0300-0605 (Print)
IS  - 0300-0605 (Linking)
VI  - 48
IP  - 6
DP  - 2020 Jun
TI  - Tetrahydropalmatine has a therapeutic effect in a lipopolysaccharide-induced 
      disseminated intravascular coagulation model.
PG  - 300060519889430
LID - 10.1177/0300060519889430 [doi]
LID - 0300060519889430
AB  - OBJECTIVES: The aim of this study was to determine the therapeutic effects of 
      tetrahydropalmatine (Tet) on disseminated intravascular coagulation (DIC) by 
      exploring the role of Tet using a lipopolysaccharide (LPS)-induced DIC model. 
      Methods/Materials: We established a mouse DIC model by injecting LPS. 
      Hematoxylin-eosin (HE) staining was performed to detect liver and kidney damage. 
      Blood samples were obtained to determine liver and kidney injury indexes, 
      coagulation indexes, and inflammatory cytokines. An in vitro cell inflammation 
      model was also established. Tumor necrosis factor-alpha (TNF-alpha) levels and nuclear 
      factor kappa B (NF-kappaB) signaling pathway activation were determined by western 
      blot. RESULT: Tet ameliorated the damage to organ tissues, improved coagulation 
      indexes, and reduced the inflammatory cytokine production in LPS-induced mouse 
      DIC. Tet also inhibited TNF-alpha expression by suppressing NF-kappaB signaling pathway 
      activation in an in vitro LPS model using RAW 264.7 macrophages. CONCLUSIONS: Tet 
      has a mitigating and therapeutic effect on the LPS-induced DIC model via 
      anticoagulant and anti-inflammatory effects, showing its potential as an adjunct 
      to DIC treatment.
FAU - Zhi, Lin
AU  - Zhi L
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Yang, Shangqi
AU  - Yang S
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Chen, Jiekun
AU  - Chen J
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Lu, Yuli
AU  - Lu Y
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Chen, Jiahong
AU  - Chen J
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Qin, Zixi
AU  - Qin Z
AD  - Department of Pharmacology, Medical College, Jinan University, Guangzhou, China.
FAU - Tang, Xiao-Mei
AU  - Tang XM
AUID- ORCID: 0000-0001-8652-3942
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20191212
PL  - England
TA  - J Int Med Res
JT  - The Journal of international medical research
JID - 0346411
RN  - 0 (Berberine Alkaloids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 3X69CO5I79 (tetrahydropalmatine)
SB  - IM
MH  - Animals
MH  - *Berberine Alkaloids/pharmacology/therapeutic use
MH  - *Disseminated Intravascular Coagulation/chemically induced/drug therapy
MH  - Lipopolysaccharides/toxicity
MH  - Mice
MH  - NF-kappa B/genetics
MH  - Tumor Necrosis Factor-alpha
PMC - PMC7327433
OTO - NOTNLM
OT  - Disseminated intravascular coagulation (DIC)
OT  - NF-kappaB
OT  - anti-inflammatory
OT  - lipopolysaccharide (LPS)
OT  - macrophages
OT  - tetrahydropalmatine (Tet)
OT  - tumor necrosis factor-alpha (TNF-alpha)
EDAT- 2019/12/14 06:00
MHDA- 2021/05/15 06:00
PMCR- 2019/12/12
CRDT- 2019/12/14 06:00
PHST- 2019/12/14 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2019/12/14 06:00 [entrez]
PHST- 2019/12/12 00:00 [pmc-release]
AID - 10.1177_0300060519889430 [pii]
AID - 10.1177/0300060519889430 [doi]
PST - ppublish
SO  - J Int Med Res. 2020 Jun;48(6):300060519889430. doi: 10.1177/0300060519889430. 
      Epub 2019 Dec 12.