PMID- 31831027 OWN - NLM STAT- MEDLINE DCOM- 20200428 LR - 20200428 IS - 1743-422X (Electronic) IS - 1743-422X (Linking) VI - 16 IP - 1 DP - 2019 Dec 12 TI - Endothelial IL-8 induced by porcine circovirus type 2 affects dendritic cell maturation and antigen-presenting function. PG - 154 LID - 10.1186/s12985-019-1256-z [doi] LID - 154 AB - BACKGROUND: Porcine circovirus (PCV) disease caused by PCV type 2 (PCV2) is mainly attributed to immunosuppression and immune damage. PCV2 can infect vascular endothelial cells and induce high expression of endothelial IL-8. Dendritic cells (DCs), as professional antigen-presenting cells, can not only present antigens but also activate naive T-cells, causing an immune response. METHODS: To demonstrate whether endothelial IL-8 is the main factor inhibiting the maturation and related functions of dendritic cells during PCV2 infection, monocyte-derived DCs (MoDCs) and porcine iliac artery endothelial cells (PIECs) processed by different methods were co-cultured in two ways. Flow cytometry, molecular probe labeling, fluorescence quantitative PCR, and the MTS assay were used to detect the changes in related functions and molecules of MoDCs. RESULTS: Compared to those in the PIEC-DC group, the endothelial IL-8 upregulation co-culture group showed significantly lower double-positive rates for CD80/86 and MHC-II of MoDCs and significantly increased endocytosis of MoDCs. Meanwhile, the adhesion rate and average fluorescence intensity of MoDCs were significantly downregulated in migration and adhesion experiments. Furthermore, the MHC-I and LAMP7 mRNA levels in MoDCs and the proliferation of MoDC-stimulated T-cells were markedly reduced. However, the changes in MoDCs of the endothelial IL-8 downregulation co-culture group were the opposite. CONCLUSIONS: PCV2-induced endothelial IL-8 reduces the adhesion and migration ability of MoDCs, resulting in a decreased maturation rate of MoDCs, and further inhibits antigen presentation by DCs. These results may explain the immunosuppressive mechanism of PCV2 from the perspective of the interaction between endothelial cells and DCs in vitro. FAU - Liu, Shiyu AU - Liu S AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Li, Qiuming AU - Li Q AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Qiao, Jinzeng AU - Qiao J AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Wang, Jianfang AU - Wang J AD - Beijing Key Laboratory of TCVM, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Cui, Defeng AU - Cui D AD - Beijing Key Laboratory of TCVM, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Gu, Kewei AU - Gu K AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. FAU - Zhou, Shuanghai AU - Zhou S AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. shhaizhou@sina.com. FAU - Li, Huanrong AU - Li H AUID- ORCID: 0000-0002-0120-0616 AD - College of Animal Science and Technology, Beijing University of Agriculture, No. 7 Beinong Road, Changping District, Beijing, 102206, China. lihuanrong1@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20191212 PL - England TA - Virol J JT - Virology journal JID - 101231645 RN - 0 (Immunologic Factors) RN - 0 (Interleukin-8) SB - IM MH - Animals MH - Antigen-Presenting Cells/*immunology/physiology MH - *Cell Differentiation MH - Cells, Cultured MH - Circovirus/growth & development/*immunology MH - Coculture Techniques MH - Dendritic Cells/*immunology/physiology MH - Endothelial Cells/metabolism/*virology MH - Immunologic Factors/*metabolism MH - Interleukin-8/*metabolism MH - Swine PMC - PMC6909562 OTO - NOTNLM OT - Antigen presentation OT - Endothelial IL-8 OT - Maturation OT - Monocyte-derived dendritic cells OT - Porcine circovirus type 2 COIS- The authors declare there are no conflicts of interest in relation to this manuscript. EDAT- 2019/12/14 06:00 MHDA- 2020/04/29 06:00 PMCR- 2019/12/12 CRDT- 2019/12/14 06:00 PHST- 2019/07/23 00:00 [received] PHST- 2019/11/21 00:00 [accepted] PHST- 2019/12/14 06:00 [entrez] PHST- 2019/12/14 06:00 [pubmed] PHST- 2020/04/29 06:00 [medline] PHST- 2019/12/12 00:00 [pmc-release] AID - 10.1186/s12985-019-1256-z [pii] AID - 1256 [pii] AID - 10.1186/s12985-019-1256-z [doi] PST - epublish SO - Virol J. 2019 Dec 12;16(1):154. doi: 10.1186/s12985-019-1256-z.