PMID- 31833006
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20201212
IS  - 1678-4405 (Electronic)
IS  - 1517-8382 (Print)
IS  - 1517-8382 (Linking)
VI  - 51
IP  - 1
DP  - 2020 Mar
TI  - Antifungal activity of Copaiba resin oil in solution and nanoemulsion against 
      Paracoccidioides spp.
PG  - 125-134
LID - 10.1007/s42770-019-00201-3 [doi]
AB  - Paracoccidioidomycosis (PCM) is a disease caused by fungi of the genus 
      Paracoccidioides. The disease is responsible for high rates of premature deaths 
      and socioeconomic repercussions. The limitations of antifungal agents against PCM 
      have motivated the search for new compounds. In our ongoing exploration of 
      Cerrado plants as potential sources of new antifungal agents, we selected 
      Copaifera langsdorffii oil (Copaiba resin oil) in order to explore its bioactive 
      potential and test a formulation to increase oil stability and solubilization 
      employing Pluronic F-127 to obtain the nanoemulsion of the oil. We aim at testing 
      both Copaiba resin oil and its nanoemulsion against four species of the 
      Paracoccidioides genus. We performed cytotoxicity test in Balb/C3T3 cells, 
      hemolytic activity and interaction of Copaiba resin oil and Copaiba resin oil 
      nanoemulsion (CopaPlu) with the antifungal agents such as amphotericin B, 
      co-trimoxazole, and itraconazole. Moreover, the Copaiba resin oil was analyzed by 
      mass spectrometry to identify its chemical profile. Eventually, a new methodology 
      to prepare the nanoemulsion is presented. The Copaiba resin oil and CopaPlu 
      nanoemulsion inhibited Paracoccidioides sp. growth efficiently, and no 
      cytotoxicity or hemolytic effect was observed at minimum inhibitory concentration 
      (MIC). When combined with amphotericin B, Copaiba resin oil and its nanoemulsion 
      showed an additive effect with reduction of MIC values. The Copaiba resin oil and 
      CopaPlu nanoemulsion is a promising antifungal agent against Paracoccidioides.
FAU - do Carmo Silva, Livia
AU  - do Carmo Silva L
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Goiania, Goias, 74690-900, Brazil.
FAU - Miranda, Meire Ane Costa Miguel
AU  - Miranda MACM
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Goiania, Goias, 74690-900, Brazil.
FAU - de Freitas, Julianna Veiga
AU  - de Freitas JV
AD  - Laboratory of Colloids and Nanostructured Materials, Institute of Chemistry, 
      Federal University of Goias, Goiania, Goias, Brazil.
FAU - Ferreira, Sarah Fernanda Araujo
AU  - Ferreira SFA
AD  - Laboratory of Colloids and Nanostructured Materials, Institute of Chemistry, 
      Federal University of Goias, Goiania, Goias, Brazil.
FAU - de Oliveira Lima, Emilia Celma
AU  - de Oliveira Lima EC
AD  - Laboratory of Colloids and Nanostructured Materials, Institute of Chemistry, 
      Federal University of Goias, Goiania, Goias, Brazil.
FAU - de Oliveira, Cecilia Maria Alves
AU  - de Oliveira CMA
AD  - Laboratory of Natural Products, Institute of Chemistry, Federal University of 
      Goias, Goiania, Goias, Brazil.
FAU - Kato, Lucilia
AU  - Kato L
AD  - Laboratory of Natural Products, Institute of Chemistry, Federal University of 
      Goias, Goiania, Goias, Brazil.
FAU - Terezan, Ana Paula
AU  - Terezan AP
AD  - Laboratory of Natural Products, Institute of Chemistry, Federal University of 
      Goias, Goiania, Goias, Brazil.
FAU - Rodriguez, Anselmo Fortunato Ruiz
AU  - Rodriguez AFR
AD  - Laboratory of Nanobiotechnology, Federal University of Acre, Rio Branco, Acre, 
      Brazil.
FAU - Faria, Fernando Sergio Escocio Drumond Viana
AU  - Faria FSEDV
AD  - Laboratory of Nanobiotechnology, Federal University of Acre, Rio Branco, Acre, 
      Brazil.
FAU - de Almeida Soares, Celia Maria
AU  - de Almeida Soares CM
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Goiania, Goias, 74690-900, Brazil.
FAU - Pereira, Maristela
AU  - Pereira M
AUID- ORCID: 0000-0002-5482-8036
AD  - Laboratory of Molecular Biology, Institute of Biological Sciences, Federal 
      University of Goias, Goiania, Goias, 74690-900, Brazil. maristela.ufg@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20191212
PL  - Brazil
TA  - Braz J Microbiol
JT  - Brazilian journal of microbiology : [publication of the Brazilian Society for 
      Microbiology]
JID - 101095924
RN  - 0 (Antifungal Agents)
RN  - 0 (Emulsions)
RN  - 0 (Oils, Volatile)
RN  - 0 (Plant Oils)
SB  - IM
MH  - Animals
MH  - Antifungal Agents/*pharmacology
MH  - Cell Line
MH  - Emulsions/chemistry/*pharmacology
MH  - Fabaceae/*chemistry
MH  - Fibroblasts/drug effects
MH  - Mass Spectrometry
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - Nanoparticles/*chemistry
MH  - Oils, Volatile/pharmacology
MH  - Paracoccidioides/*drug effects
MH  - Plant Oils/chemistry/*pharmacology
PMC - PMC7058720
OTO - NOTNLM
OT  - Antifungal
OT  - Copaifera langsdorffii
OT  - Copaiba resin oil
OT  - Nanoemulsion
OT  - Paracoccidioides spp.
EDAT- 2019/12/14 06:00
MHDA- 2020/11/11 06:00
PMCR- 2020/12/12
CRDT- 2019/12/14 06:00
PHST- 2019/05/10 00:00 [received]
PHST- 2019/11/27 00:00 [accepted]
PHST- 2019/12/14 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2019/12/14 06:00 [entrez]
PHST- 2020/12/12 00:00 [pmc-release]
AID - 10.1007/s42770-019-00201-3 [pii]
AID - 201 [pii]
AID - 10.1007/s42770-019-00201-3 [doi]
PST - ppublish
SO  - Braz J Microbiol. 2020 Mar;51(1):125-134. doi: 10.1007/s42770-019-00201-3. Epub 
      2019 Dec 12.