PMID- 31833032
OWN - NLM
STAT- MEDLINE
DCOM- 20200603
LR  - 20211204
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 47
IP  - 1
DP  - 2020 Jan
TI  - Frequency of dendritic cell subsets and ILT3, ILT4 gene expression in two 
      different immunosuppressive protocols in kidney transplant recipients. A cohort 
      report.
PG  - 123-128
LID - 10.1007/s11033-019-05113-x [doi]
AB  - Dendritic cells (DCs) have a major role in the initiation of an immune response 
      and Immunoglobulin-like transcript 3&4 (ILT3&ILT4) are inhibitory receptors that 
      induce tolerance in DCs. Recent studies show that immunosuppressive agents affect 
      frequency of DCs. Herein, we compared the effect of mycophenolate mofetil (MMF) 
      and sirolimus (SRL) in tacrolimus (TAC)-based immunosuppression on DC subsets 
      frequency and ILT3/ILT4 gene expression in kidney transplant recipients. We 
      enrolled 24 adult transplant recipients who received MMF/TAC (n = 14) or SRL/TAC 
      (n = 10). Peripheral blood samples were obtained from recipients, 24-48 h before 
      transplantation and 4 months after transplantation. The frequency of DC subsets 
      was analyzed by flow cytometry and gene expression of ILT3/ILT4 were estimated by 
      real-time PCR. Our results showed that MMF vs. SRL treated recipient showed an 
      increase in pDC % with increased in the expression of ILT3/ILT4 which is in favor 
      of better allograft survival; However, for confirming the results of this 
      preliminary study, a cohort study with larger sample size is necessary.
FAU - Karimi, Mozhdeh
AU  - Karimi M
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
AD  - Molecular Immunology Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Ahmadpoor, Pedram
AU  - Ahmadpoor P
AD  - Chronic Kidney Disease Research Center, Labbafinejad Medical Center, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Nafar, Mohsen
AU  - Nafar M
AD  - Chronic Kidney Disease Research Center, Labbafinejad Medical Center, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Pourrezagholi, Fatemeh
AU  - Pourrezagholi F
AD  - Chronic Kidney Disease Research Center, Labbafinejad Medical Center, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran.
FAU - Jamali, Saeideh
AU  - Jamali S
AD  - Molecular Immunology Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Eteghadi, Atefeh
AU  - Eteghadi A
AD  - Molecular Immunology Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Yekaninejad, Mir Saeed
AU  - Yekaninejad MS
AD  - Department of Epidemiology and Biostatistics, School of Public Health, Tehran 
      University of Medical Sciences, Tehran, Iran.
FAU - Amirzargar, Ali Akbar
AU  - Amirzargar AA
AD  - Department of Immunology, School of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran. amirzara@sina.tums.ac.ir.
AD  - Molecular Immunology Research Center, Tehran University of Medical Sciences, 
      Tehran, Iran. amirzara@sina.tums.ac.ir.
LA  - eng
GR  - 30074/tehran university of medical sciences/
PT  - Journal Article
DEP - 20191213
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Immunosuppressive Agents)
RN  - 0 (LILRB2 protein, human)
RN  - 0 (LILRB4 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Immunologic)
RN  - HU9DX48N0T (Mycophenolic Acid)
RN  - W36ZG6FT64 (Sirolimus)
RN  - WM0HAQ4WNM (Tacrolimus)
SB  - IM
MH  - Adult
MH  - Blood Cell Count
MH  - Cohort Studies
MH  - Dendritic Cells/*cytology/drug effects/metabolism
MH  - Female
MH  - Gene Expression/drug effects
MH  - Humans
MH  - Immunosuppression Therapy/adverse effects/methods
MH  - Immunosuppressive Agents/*pharmacology/therapeutic use
MH  - Kidney Failure, Chronic/blood/immunology/therapy
MH  - *Kidney Transplantation/methods
MH  - Male
MH  - Membrane Glycoproteins/*genetics/metabolism
MH  - Middle Aged
MH  - Mycophenolic Acid/pharmacology/therapeutic use
MH  - Receptors, Immunologic/*genetics/metabolism
MH  - Sirolimus/pharmacology/therapeutic use
MH  - Tacrolimus/pharmacology/therapeutic use
MH  - *Transplant Recipients
MH  - *Transplantation Conditioning/adverse effects/methods
MH  - Young Adult
OTO - NOTNLM
OT  - Dendritic cells
OT  - ILT3
OT  - ILT4
OT  - Immunoglobulin-like transcript
OT  - Kidney transplantation
OT  - Micofenolat mofetil
OT  - Sirolimus
OT  - Tacrolimus
EDAT- 2019/12/14 06:00
MHDA- 2020/06/04 06:00
CRDT- 2019/12/14 06:00
PHST- 2019/05/20 00:00 [received]
PHST- 2019/09/26 00:00 [accepted]
PHST- 2019/12/14 06:00 [pubmed]
PHST- 2020/06/04 06:00 [medline]
PHST- 2019/12/14 06:00 [entrez]
AID - 10.1007/s11033-019-05113-x [pii]
AID - 10.1007/s11033-019-05113-x [doi]
PST - ppublish
SO  - Mol Biol Rep. 2020 Jan;47(1):123-128. doi: 10.1007/s11033-019-05113-x. Epub 2019 
      Dec 13.