PMID- 31833096
OWN - NLM
STAT- MEDLINE
DCOM- 20200213
LR  - 20200213
IS  - 1520-6327 (Electronic)
IS  - 0739-4462 (Linking)
VI  - 103
IP  - 3
DP  - 2020 Mar
TI  - Development of efficient RNAi in Nezara viridula for use in insecticide target 
      discovery.
PG  - e21650
LID - 10.1002/arch.21650 [doi]
AB  - Stink bugs are an emerging pest in many regions of the world but their molecular 
      biology is still poorly understood. While several transcriptomes are available, 
      the lack of validated gene manipulation tools like RNA interference (RNAi) in 
      species such as the southern green stinkbug Nezara viridula precludes the 
      characterization of individual genes in vivo. Such tools are particularly useful 
      in performing high-throughput screens to search for essential genes that can be 
      prioritized as potential insecticide targets. Here, we developed and optimized an 
      efficient RNAi in N. viridula for use in insecticide target discovery and beyond. 
      The visible marker Sex combs reduced and the essential gene Actin were used to 
      verify the usability and efficiency of RNAi by microinjection at both the adult 
      and nymphal stages, respectively, with nymphal approach presenting significant 
      advantages. Following validation, RNAi was then used to measure lethality 
      following the knockdown (KD) of two genes that are known insecticide targets, 
      Chitin synthase, and Acetyl-CoA carboxylase. The KD of each gene resulted in >75% 
      corrected mortality. These results indicate that RNAi is an effective tool in N. 
      viridula and set a benchmark to evaluate potential targets in future RNAi screens 
      aimed at insecticide target discovery.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Riga, Maria
AU  - Riga M
AD  - Institute of Molecular Biology and Biotechnology, Foundation for Research and 
      Technology-Hellas, Heraklion, Greece.
FAU - Denecke, Shane
AU  - Denecke S
AUID- ORCID: 0000-0002-7291-1394
AD  - Institute of Molecular Biology and Biotechnology, Foundation for Research and 
      Technology-Hellas, Heraklion, Greece.
FAU - Livadaras, Ioannis
AU  - Livadaras I
AD  - Institute of Molecular Biology and Biotechnology, Foundation for Research and 
      Technology-Hellas, Heraklion, Greece.
FAU - Geibel, Sven
AU  - Geibel S
AD  - Division Crop Science, Research and Development, Bayer AG, Monheim, Germany.
FAU - Nauen, Ralf
AU  - Nauen R
AD  - Division Crop Science, Research and Development, Bayer AG, Monheim, Germany.
FAU - Vontas, John
AU  - Vontas J
AUID- ORCID: 0000-0002-8704-2574
AD  - Institute of Molecular Biology and Biotechnology, Foundation for Research and 
      Technology-Hellas, Heraklion, Greece.
AD  - Department of Crop Science, Pesticide Science Laboratory, Agricultural University 
      of Athens, Athens, Greece.
LA  - eng
GR  - Bayer AG/
PT  - Journal Article
DEP - 20191213
PL  - United States
TA  - Arch Insect Biochem Physiol
JT  - Archives of insect biochemistry and physiology
JID - 8501752
RN  - 0 (Insect Proteins)
RN  - 0 (Insecticides)
SB  - IM
MH  - Animals
MH  - Gene Expression Regulation/drug effects
MH  - Heteroptera/*drug effects/*metabolism
MH  - Insect Proteins/genetics/metabolism
MH  - Insecticides/*pharmacology
MH  - *RNA Interference
OTO - NOTNLM
OT  - Nezara viridula
OT  - RNAi
OT  - insecticide
OT  - target
EDAT- 2019/12/14 06:00
MHDA- 2020/02/14 06:00
CRDT- 2019/12/14 06:00
PHST- 2019/10/03 00:00 [received]
PHST- 2019/11/19 00:00 [revised]
PHST- 2019/11/21 00:00 [accepted]
PHST- 2019/12/14 06:00 [pubmed]
PHST- 2020/02/14 06:00 [medline]
PHST- 2019/12/14 06:00 [entrez]
AID - 10.1002/arch.21650 [doi]
PST - ppublish
SO  - Arch Insect Biochem Physiol. 2020 Mar;103(3):e21650. doi: 10.1002/arch.21650. 
      Epub 2019 Dec 13.