PMID- 31835577
OWN - NLM
STAT- MEDLINE
DCOM- 20200924
LR  - 20200924
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 11
IP  - 12
DP  - 2019 Dec 10
TI  - Fatty Acids Regulate Porcine Reproductive and Respiratory Syndrome Virus 
      Infection via the AMPK-ACC1 Signaling Pathway.
LID - 10.3390/v11121145 [doi]
LID - 1145
AB  - Lipids play a crucial role in the replication of porcine reproductive and 
      respiratory syndrome virus (PRRSV), a porcine virus that is endemic throughout 
      the world. However, little is known about the effect of fatty acids (FAs), a type 
      of vital lipid, on PRRSV infection. In this study, we found that treatment with a 
      FA biosynthetic inhibitor significantly inhibited PRRSV propagation, indicating 
      the necessity of FAs for optimal replication of PRRSV. Further study revealed 
      that 5'-adenosine monophosphate (AMP)-activated protein kinase (AMPK), a key 
      kinase antagonizing FA biosynthesis, was strongly activated by PRRSV and the 
      pharmacological activator of AMPK exhibited anti-PRRSV activity. Additionally, we 
      found that acetyl-CoA carboxylase 1 (ACC1), the first rate-limiting enzyme in the 
      FA biosynthesis pathway, was phosphorylated (inactive form) by PRRSV-activated 
      AMPK, and active ACC1 was required for PRRSV proliferation, suggesting that the 
      PRRSV infection induced the activation of the AMPK-ACC1 pathway, which was not 
      conducive to PRRSV replication. This work provides new evidence about the 
      mechanisms involved in host lipid metabolism during PRRSV infection and 
      identifies novel potential antiviral targets for PRRSV.
FAU - Long, Siwen
AU  - Long S
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Zhou, Yanrong
AU  - Zhou Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Bai, Dongcheng
AU  - Bai D
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Hao, Wanjun
AU  - Hao W
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Zheng, Bohan
AU  - Zheng B
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Xiao, Shaobo
AU  - Xiao S
AUID- ORCID: 0000-0003-0023-9188
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
FAU - Fang, Liurong
AU  - Fang L
AUID- ORCID: 0000-0003-1406-6409
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, China.
AD  - The Key Laboratory of Preventive Veterinary Medicine in Hubei Province, 
      Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, 
      China.
AD  - Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong 
      Agricultural University, 1 Shi-zi-shan Street, Wuhan 430070, Hubei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191210
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Fatty Acids)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
SB  - IM
MH  - AMP-Activated Protein Kinases/*metabolism
MH  - Acetyl-CoA Carboxylase/*metabolism
MH  - Animals
MH  - Biosynthetic Pathways/drug effects
MH  - Fatty Acids/*metabolism
MH  - *Host-Pathogen Interactions
MH  - Models, Biological
MH  - Porcine Reproductive and Respiratory Syndrome/*metabolism/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Signal Transduction
MH  - Swine
MH  - Virus Replication
PMC - PMC6950460
OTO - NOTNLM
OT  - AMP-activated protein kinase (AMPK)
OT  - acetyl-CoA carboxylase 1 (ACC1)
OT  - fatty acid
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
COIS- The authors declare no conflicts of interest.
EDAT- 2019/12/15 06:00
MHDA- 2020/09/25 06:00
PMCR- 2019/12/01
CRDT- 2019/12/15 06:00
PHST- 2019/10/25 00:00 [received]
PHST- 2019/12/06 00:00 [revised]
PHST- 2019/12/08 00:00 [accepted]
PHST- 2019/12/15 06:00 [entrez]
PHST- 2019/12/15 06:00 [pubmed]
PHST- 2020/09/25 06:00 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - v11121145 [pii]
AID - viruses-11-01145 [pii]
AID - 10.3390/v11121145 [doi]
PST - epublish
SO  - Viruses. 2019 Dec 10;11(12):1145. doi: 10.3390/v11121145.