PMID- 31835644
OWN - NLM
STAT- MEDLINE
DCOM- 20200427
LR  - 20200427
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 24
DP  - 2019 Dec 10
TI  - Effect of Methionine Diet on Metabolic and Histopathological Changes of Rat 
      Hippocampus.
LID - 10.3390/ijms20246234 [doi]
LID - 6234
AB  - Hyperhomocysteinemia (hHcy) is regarded as an independent and strong risk factor 
      for cerebrovascular diseases, stroke, and dementias. The hippocampus has a 
      crucial role in spatial navigation and memory processes and is being constantly 
      studied for neurodegenerative disorders. We used a moderate methionine (Met) diet 
      at a dose of 2 g/kg of animal weight/day in duration of four weeks to induce mild 
      hHcy in adult male Wistar rats. A novel approach has been used to explore the 
      hippocampal metabolic changes using proton magnetic resonance spectroscopy (1H 
      MRS), involving a 7T MR scanner in combination with histochemical and 
      immunofluorescence analysis. We found alterations in the metabolic profile, as 
      well as remarkable histo-morphological changes such as an increase of hippocampal 
      volume, alterations in number and morphology of astrocytes, neurons, and their 
      processes in the selective vulnerable brain area of animals treated with a 
      Met-enriched diet. Results of both methodologies suggest that the mild hHcy 
      induced by Met-enriched diet alters volume, histo-morphological pattern, and 
      metabolic profile of hippocampal brain area, which might eventually endorse the 
      neurodegenerative processes.
FAU - Kovalska, Maria
AU  - Kovalska M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Hnilicova, Petra
AU  - Hnilicova P
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Kalenska, Dagmar
AU  - Kalenska D
AD  - Department of Anatomy, Jessenius Faculty of Medicine, Comenius University in 
      Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Tothova, Barbara
AU  - Tothova B
AD  - Department of Molecular Medicine, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
FAU - Adamkov, Marian
AU  - Adamkov M
AD  - Department of Histology and Embryology, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
FAU - Lehotsky, Jan
AU  - Lehotsky J
AD  - Department of Neuroscience, Biomedical Center Martin, Jessenius Faculty of 
      Medicine, Comenius University in Bratislava, 03601 Martin, Slovakia.
AD  - Department of Medical Biochemistry, Jessenius Faculty of Medicine, Comenius 
      University in Bratislava, 03601 Martin, Slovakia.
LA  - eng
GR  - APVV 15/0107/Agentura na Podporu Vyskumu a Vyvoja/
GR  - No.128/16/Vedecka Grantova Agentura MSVVaS SR a SAV/
GR  - "Identification of novel markers in diagnostic panel of neurological diseases" 
      code: 26220220114/ASFEU/
PT  - Journal Article
DEP - 20191210
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Hippocampus/*diagnostic imaging/drug effects/metabolism
MH  - Hyperhomocysteinemia/chemically induced/*diagnostic imaging/metabolism
MH  - Male
MH  - Metabolome/*drug effects
MH  - Methionine/*adverse effects
MH  - Organ Size/drug effects
MH  - Proton Magnetic Resonance Spectroscopy
MH  - Rats
MH  - Rats, Wistar
PMC - PMC6941024
OTO - NOTNLM
OT  - 1H MRS
OT  - hippocampus
OT  - hyperhomocysteinemia
OT  - methionine diet
OT  - neurodegeneration
COIS- The authors declare no conflict of interest.
EDAT- 2019/12/15 06:00
MHDA- 2020/04/28 06:00
PMCR- 2019/12/01
CRDT- 2019/12/15 06:00
PHST- 2019/11/14 00:00 [received]
PHST- 2019/12/05 00:00 [revised]
PHST- 2019/12/06 00:00 [accepted]
PHST- 2019/12/15 06:00 [entrez]
PHST- 2019/12/15 06:00 [pubmed]
PHST- 2020/04/28 06:00 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - ijms20246234 [pii]
AID - ijms-20-06234 [pii]
AID - 10.3390/ijms20246234 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Dec 10;20(24):6234. doi: 10.3390/ijms20246234.