PMID- 31835792
OWN - NLM
STAT- MEDLINE
DCOM- 20201026
LR  - 20201026
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 11
IP  - 12
DP  - 2019 Dec 11
TI  - A Rapid Extraction Method Combined with a Monoclonal Antibody-Based Immunoassay 
      for the Detection of Amatoxins.
LID - 10.3390/toxins11120724 [doi]
LID - 724
AB  - Amatoxins (AMAs) are lethal toxins found in a variety of mushroom species. 
      Detection methods are needed to determine the occurrence of AMAs in mushroom 
      species suspected in mushroom poisonings. In this manuscript, we report the 
      generation of novel monoclonal antibodies (mAbs, AMA9G3 and AMA9C12) and the 
      development of a competitive, enzyme-linked immunosorbent assay (cELISA) that is 
      sensitive at 1 ng mL(-1) and shows selectivity for alpha-amanitin (alpha-AMA) and 
      gamma-amanitin (gamma-AMA), and less for beta-amanitin (beta-AMA). In order to decrease the 
      overall time needed for analysis, the extraction procedure for mushrooms was also 
      simplified. A rapid (1 min) extraction procedure of AMAs using solvents as simple 
      as water alone was successfully demonstrated using Amanita mushrooms. Together, 
      the extraction method and the mAb-based ELISA represent a simple and rapid method 
      that readily detects AMAs extracted from mushroom samples.
FAU - Bever, Candace S
AU  - Bever CS
AD  - Foodborne Toxin Detection and Prevention Unit, Western Regional Research Center, 
      Agricultural Research Service, United States Department of Agriculture, 800 
      Buchanan Street, Albany, CA 94710, USA.
FAU - Hnasko, Robert M
AU  - Hnasko RM
AD  - Produce Safety and Microbiology Unit, Western Regional Research Center, 
      Agricultural Research Service, United States Department of Agriculture, 800 
      Buchanan Street, Albany, CA 94710, USA.
FAU - Cheng, Luisa W
AU  - Cheng LW
AD  - Foodborne Toxin Detection and Prevention Unit, Western Regional Research Center, 
      Agricultural Research Service, United States Department of Agriculture, 800 
      Buchanan Street, Albany, CA 94710, USA.
FAU - Stanker, Larry H
AU  - Stanker LH
AD  - Foodborne Toxin Detection and Prevention Unit, Western Regional Research Center, 
      Agricultural Research Service, United States Department of Agriculture, 800 
      Buchanan Street, Albany, CA 94710, USA.
LA  - eng
GR  - 60-2030-5-004/DHS/DHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20191211
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 0 (Amanitins)
RN  - 0 (Antibodies, Monoclonal)
RN  - 10450-60-9 (Periodic Acid)
RN  - 58250-15-0 (amatoxin)
RN  - 9013-72-3 (Hemocyanins)
RN  - B45A1BUM4Q (metaperiodate)
RN  - FV4Y0JO2CX (keyhole-limpet hemocyanin)
SB  - IM
MH  - Amanita
MH  - Amanitins/*analysis/immunology
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Female
MH  - Hemocyanins/immunology
MH  - Mice, Inbred BALB C
MH  - Periodic Acid/immunology
PMC - PMC6950293
OTO - NOTNLM
OT  - ELISA
OT  - amanitins
OT  - amatoxins
OT  - death cap mushrooms
OT  - immunoassay
OT  - monoclonal antibodies
COIS- The authors declare a competing interest. C.S.B., R.M.H., L.W.C. and L.H.S. are 
      named as inventors on a provisional patent application filed by ARS-USDA related 
      to the data presented in this work.
EDAT- 2019/12/15 06:00
MHDA- 2020/10/27 06:00
PMCR- 2019/12/01
CRDT- 2019/12/15 06:00
PHST- 2019/11/22 00:00 [received]
PHST- 2019/12/05 00:00 [revised]
PHST- 2019/12/09 00:00 [accepted]
PHST- 2019/12/15 06:00 [entrez]
PHST- 2019/12/15 06:00 [pubmed]
PHST- 2020/10/27 06:00 [medline]
PHST- 2019/12/01 00:00 [pmc-release]
AID - toxins11120724 [pii]
AID - toxins-11-00724 [pii]
AID - 10.3390/toxins11120724 [doi]
PST - epublish
SO  - Toxins (Basel). 2019 Dec 11;11(12):724. doi: 10.3390/toxins11120724.