PMID- 31836166
OWN - NLM
STAT- MEDLINE
DCOM- 20200224
LR  - 20200224
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 254
DP  - 2019 Dec
TI  - Serum concentration of homocysteine in spontaneous feline chronic kidney disease.
PG  - 105358
LID - S1090-0233(18)30368-X [pii]
LID - 10.1016/j.tvjl.2019.105358 [doi]
AB  - Serum homocysteine (Hcy) increases in people and dogs with chronic kidney disease 
      (CKD). Hyperhomocysteinemia (HHcy) has also been associated with CKD-related 
      hypertension and proteinuria. The aims of this study were to: (1) validate an 
      enzymatic method for quantification of Hcy in feline serum; (2) evaluate whether 
      HHcy was associated with the presence and severity of CKD, proteinuria or 
      hypertension; and (3) determine whether HHcy could predict disease progression. 
      The intra- and inter-assay coefficients of variation (CVs) and the recovery rates 
      of linearity under dilution and spiking recovery tests of the enzymatic method 
      were 3.1-6.7%, 11.6-12.5%, 96.9+/-5.4% and 96.9+/-5.4%, respectively. Healthy cats at 
      risk of CKD (n=17) and cats with CKD (n=19) were sampled over a 6-month period 
      (63 samples in total). Cats with CKD had significantly higher Hcy concentrations 
      (P=0.005) than cats at risk. The concentration of Hcy was higher (P=0.002) in 
      moderate-severe CKD than in mild CKD and correlated moderately with serum 
      creatinine (P<0.0001; r=0.51). The concentration of Hcy increased with the 
      magnitude of proteinuria and correlated weakly with urinary protein to creatinine 
      ratio (P=0.045; r=0.26). HHcy was not associated with hypertension. At the time 
      of enrollment, Hcy concentration was significantly higher (P=0.046) in cats that 
      developed CKD compared to cats that remained stable. The enzymatic method for Hcy 
      measurement in feline serum was precise and accurate. HHcy was relatively common 
      in cats with advanced CKD and seemed to predict disease progression, but further 
      studies are warranted.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Giraldi, M
AU  - Giraldi M
AD  - Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 
      Milan, Italy; Veterinary Teaching Hospital, University of Milan, Via 
      dell'Universita 6, 26900 Lodi, Italy. Electronic address: 
      m.giraldi.vet@gmail.com.
FAU - Paltrinieri, S
AU  - Paltrinieri S
AD  - Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 
      Milan, Italy; Veterinary Teaching Hospital, University of Milan, Via 
      dell'Universita 6, 26900 Lodi, Italy.
FAU - Curcio, C
AU  - Curcio C
AD  - Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 
      Milan, Italy.
FAU - Scarpa, P
AU  - Scarpa P
AD  - Department of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 
      Milan, Italy; Veterinary Teaching Hospital, University of Milan, Via 
      dell'Universita 6, 26900 Lodi, Italy.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20190822
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - 0LVT1QZ0BA (Homocysteine)
SB  - IM
MH  - Animals
MH  - Azotemia/blood/veterinary
MH  - Cat Diseases/*blood
MH  - Cats
MH  - Enzyme Assays/methods/*veterinary
MH  - Female
MH  - Homocysteine/*blood
MH  - Hyperhomocysteinemia/blood/*veterinary
MH  - Hypertension/blood/veterinary
MH  - Longitudinal Studies
MH  - Male
MH  - Proteinuria/blood/veterinary
MH  - Renal Insufficiency, Chronic/blood/*veterinary
OTO - NOTNLM
OT  - Cats
OT  - Chronic kidney disease
OT  - Homocysteine
OT  - Hypertension
OT  - Proteinuria
EDAT- 2019/12/15 06:00
MHDA- 2020/02/25 06:00
CRDT- 2019/12/15 06:00
PHST- 2018/07/11 00:00 [received]
PHST- 2019/08/18 00:00 [revised]
PHST- 2019/08/20 00:00 [accepted]
PHST- 2019/12/15 06:00 [entrez]
PHST- 2019/12/15 06:00 [pubmed]
PHST- 2020/02/25 06:00 [medline]
AID - S1090-0233(18)30368-X [pii]
AID - 10.1016/j.tvjl.2019.105358 [doi]
PST - ppublish
SO  - Vet J. 2019 Dec;254:105358. doi: 10.1016/j.tvjl.2019.105358. Epub 2019 Aug 22.