PMID- 31837589
OWN - NLM
STAT- MEDLINE
DCOM- 20200203
LR  - 20200203
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 110
DP  - 2020 Feb
TI  - Transcriptome analysis of human periodontal ligament fibroblasts exposed to 
      Porphyromonas gingivalis LPS.
PG  - 104632
LID - S0003-9969(19)30978-1 [pii]
LID - 10.1016/j.archoralbio.2019.104632 [doi]
AB  - OBJECTIVE: Porphyromonas gingivalis (P. gingivalis)-derived LPS is a major 
      mediator of inflammation and can promote the resorption of alveolar bone in 
      chronic periodontitis. Although the effect of P. gingivalis LPS on human 
      periodontal ligament fibroblasts (hPDLFs) was already investigated by numerous 
      studies, the change of whole transcriptional profile remains undefined. The aim 
      of this study was to investigate P. gingivalis LPS induced whole transcriptional 
      profile in hPDLFs and the expression of the genes associated with the 
      periodontitis. MATERIALS AND METHODS: RNA-seq was performed on hPDLFs treated 
      with or without P. gingivalis LPS. Moreover, the expression of selected 
      inflammatory cytokines, chemokines and matrix metalloproteinases (MMPs), which 
      contribute to periodontitis, were evaluated by quantitative RT-PCR and further 
      measured by ELISA. RESULTS: We found that an average of 12,752 genes were 
      detected among the different groups, and 1374 differentially expressed genes 
      (DEGs) were identified between groups with or without P. gingivalis LPS 
      stimulation for 24 h. However, only 36 DEGs were examined in hPDLFs exposed to P. 
      gingivalis LPS for 24 h or 72 h. Furthermore, the mRNA levels and concentrations 
      of interleukin 8 (IL-8), IL-6, monocyte chemotactic protein 1 (MCP-1), chemokine 
      (CXC motif) ligand 5 (CXCL5), MMP1 and MMP3 were significantly higher in hPDLFs 
      exposed to P. gingivalis LPS for 24 h compared to the untreated hPDLFs. 
      CONCLUSIONS: The entire transcriptional profile of P. gingivalis LPS stimulation 
      of hPDLFs was presented for the first time, which could provide an important 
      basis and experimental direction for further research into the mechanisms of 
      periodontitis.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Wu, Xi
AU  - Wu X
AD  - Department of Stomatology, Xinqiao Hospital, Army Medical University (Third 
      Military Medical University), Chongqing 400037, PR China.
FAU - Zhang, Gang
AU  - Zhang G
AD  - Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Army Medical 
      University (Third Military Medical University), Chongqing 400037, PR China.
FAU - Feng, Xiaoqian
AU  - Feng X
AD  - Department of Stomatology, Xinqiao Hospital, Army Medical University (Third 
      Military Medical University), Chongqing 400037, PR China.
FAU - Li, Pengfei
AU  - Li P
AD  - Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Army Medical 
      University (Third Military Medical University), Chongqing 400037, PR China.
FAU - Tan, Yinghui
AU  - Tan Y
AD  - Department of Oral and Maxillofacial Surgery, Xinqiao Hospital, Army Medical 
      University (Third Military Medical University), Chongqing 400037, PR China. 
      Electronic address: tanyh1962@outlook.com.
LA  - eng
PT  - Journal Article
DEP - 20191205
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Cells, Cultured
MH  - Fibroblasts
MH  - *Gene Expression Profiling
MH  - Humans
MH  - Inflammation
MH  - Lipopolysaccharides
MH  - *Periodontal Ligament/metabolism
MH  - Periodontitis
MH  - *Porphyromonas gingivalis/pathogenicity
OTO - NOTNLM
OT  - Chemokines
OT  - Inflammatory cytokines
OT  - MMPs
OT  - P. gingivalis LPS
OT  - RNA-seq
OT  - hPDLFs
EDAT- 2019/12/15 06:00
MHDA- 2020/02/06 06:00
CRDT- 2019/12/15 06:00
PHST- 2019/09/24 00:00 [received]
PHST- 2019/11/09 00:00 [revised]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2019/12/15 06:00 [pubmed]
PHST- 2020/02/06 06:00 [medline]
PHST- 2019/12/15 06:00 [entrez]
AID - S0003-9969(19)30978-1 [pii]
AID - 10.1016/j.archoralbio.2019.104632 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2020 Feb;110:104632. doi: 10.1016/j.archoralbio.2019.104632. Epub 
      2019 Dec 5.