PMID- 31838218
OWN - NLM
STAT- MEDLINE
DCOM- 20210125
LR  - 20210125
IS  - 1872-9738 (Electronic)
IS  - 0892-0362 (Linking)
VI  - 77
DP  - 2020 Jan-Feb
TI  - Magnetic Resonance Imaging and Micro-Computed Tomography reveal brain 
      morphological abnormalities in a mouse model of early moderate prenatal ethanol 
      exposure.
PG  - 106849
LID - S0892-0362(19)30095-9 [pii]
LID - 10.1016/j.ntt.2019.106849 [doi]
AB  - BACKGROUND: This study investigated the effects of early moderate prenatal 
      ethanol exposure (PEE) on the brain in a mouse model that mimics a scenario in 
      humans, whereby moderate daily drinking ceases after a woman becomes aware of her 
      pregnancy. METHODS: C57BL/6J pregnant mice were given 10% v/v ethanol from 
      gestational day 0-8 in the drinking water. The male offspring were used for 
      imaging. Anatomical and diffusion Magnetic Resonance Imaging were performed in 
      vivo at postnatal day 28 (P28, adolescence) and P80 (adulthood). Micro-Computed 
      Tomography was performed on fixed whole heads at P80. Tensor-based morphometry 
      (TBM) was applied to detect alterations in brain structure and voxel-based 
      morphometry (VBM) for skull morphology. Diffusion tensor and neurite orientation 
      dispersion and density imaging models were used to detect microstructural 
      changes. Neurofilament (NF) immunohistochemistry was used to validate findings by 
      in vivo diffusion MRI. RESULTS: TBM showed that PEE mice exhibited a 
      significantly smaller third ventricle at P28 (family-wise error rate (FWE), p < 
      0.05). All other macro-structural alterations did not survive FWE corrections but 
      when displayed with an uncorrected p < 0.005 showed multiple regional volume 
      reductions and expansions, more prominently in the right hemisphere. PEE-induced 
      gross volume changes included a bigger thalamus, hypothalamus and ventricles at 
      P28, and bigger total brain volumes at both P28 and P80 (2-sample t-tests). 
      Disproportionately smaller olfactory bulbs following PEE were revealed at both 
      time-points. No alterations in diffusion parameters were detected, but PEE 
      animals exhibited reduced NF positive staining in the thalamus and striatum and 
      greater bone density in various skull regions. CONCLUSION: Our results show that 
      early moderate PEE can cause alterations in the brain that are detectable during 
      development and adulthood.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Nguyen, Van T
AU  - Nguyen VT
AD  - Centre for Advanced Imaging, The University of Queensland, Brisbane, Queensland, 
      Australia; Hanoi University of Science and Technology, Hanoi, Viet Nam.
FAU - Tieng, Quang M
AU  - Tieng QM
AD  - Centre for Advanced Imaging, The University of Queensland, Brisbane, Queensland, 
      Australia.
FAU - Mardon, Karine
AU  - Mardon K
AD  - Centre for Advanced Imaging, The University of Queensland, Brisbane, Queensland, 
      Australia; National Imaging Facility, Brisbane, Queensland, Australia.
FAU - Zhang, Christine
AU  - Zhang C
AD  - Mater Research Institute, The University of Queensland, Brisbane, Queensland, 
      Australia.
FAU - Chong, Suyinn
AU  - Chong S
AD  - Mater Research Institute, The University of Queensland, Brisbane, Queensland, 
      Australia; Translational Research Institute, Brisbane, Queensland, Australia.
FAU - Galloway, Graham J
AU  - Galloway GJ
AD  - Centre for Advanced Imaging, The University of Queensland, Brisbane, Queensland, 
      Australia; Translational Research Institute, Brisbane, Queensland, Australia; 
      National Imaging Facility, Brisbane, Queensland, Australia.
FAU - Kurniawan, Nyoman D
AU  - Kurniawan ND
AD  - Centre for Advanced Imaging, The University of Queensland, Brisbane, Queensland, 
      Australia. Electronic address: n.kurniawan@uq.edu.au.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191212
PL  - United States
TA  - Neurotoxicol Teratol
JT  - Neurotoxicology and teratology
JID - 8709538
RN  - 3K9958V90M (Ethanol)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Atrophy/pathology
MH  - Brain/metabolism/*pathology
MH  - Diffusion Magnetic Resonance Imaging
MH  - Ethanol/*adverse effects
MH  - Female
MH  - Image Processing, Computer-Assisted
MH  - Intermediate Filaments/metabolism
MH  - Male
MH  - Mice
MH  - Neurites/pathology
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/chemically induced/metabolism/*pathology
MH  - Skull/*abnormalities
MH  - Third Ventricle/pathology
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Computed tomography
OT  - Fetal alcohol spectrum disorders
OT  - MRI
OT  - Morphometry
OT  - Mouse brain
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2019/12/16 06:00
MHDA- 2021/01/26 06:00
CRDT- 2019/12/16 06:00
PHST- 2019/07/15 00:00 [received]
PHST- 2019/11/01 00:00 [revised]
PHST- 2019/12/04 00:00 [accepted]
PHST- 2019/12/16 06:00 [pubmed]
PHST- 2021/01/26 06:00 [medline]
PHST- 2019/12/16 06:00 [entrez]
AID - S0892-0362(19)30095-9 [pii]
AID - 10.1016/j.ntt.2019.106849 [doi]
PST - ppublish
SO  - Neurotoxicol Teratol. 2020 Jan-Feb;77:106849. doi: 10.1016/j.ntt.2019.106849. 
      Epub 2019 Dec 12.