PMID- 31839667
OWN - NLM
STAT- MEDLINE
DCOM- 20200415
LR  - 20200415
IS  - 1880-313X (Electronic)
IS  - 0388-6107 (Linking)
VI  - 40
IP  - 6
DP  - 2019
TI  - T-cell activation-inhibitory assay: a proposed novel method for screening caloric 
      restriction mimetics.
PG  - 235-241
LID - 10.2220/biomedres.40.235 [doi]
AB  - Caloric restriction (CR) is a major contributor to good health and longevity. CR 
      mimetics (CRMs) are a group of plant-derived compounds capable of inducing the 
      benefits of CR. Since a longevity gene, SIRT1, inhibits T-cell activation and 
      SIRT1 loss results in increased T-cell activation, we hypothesized that compounds 
      capable of activating SIRT1 signaling can inhibit T-cell activation and function 
      as CRMs. Thus we propose, in the present study, the application of a T-cell 
      activation-inhibitory assay to screen candidate CRMs. Well-known CRMs, such as 
      resveratrol, butein, and fisetin, suppressed the anti-CD3/CD28 antibody-induced 
      activation of mouse spleen T-cells. We next randomly assessed 68 plant-derived 
      compounds for screening novel candidate CRMs using this bioassay and found that 
      all four compounds showing IC(50) values <5 muM, such as curcumin, alpha-mangostin, 
      nobiletin, and heptamethoxyflavone, have beneficial functions for health such as 
      anti-inflammatory effect. These results suggest that the T-cell 
      activation-inhibitory assay can be used to screen candidate CRMs.
FAU - Iura, Shu
AU  - Iura S
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
FAU - Ojima, Yuusei
AU  - Ojima Y
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
FAU - Amakura, Yoshiaki
AU  - Amakura Y
AD  - Department of Pharmacognosy, College of Pharmaceutical Sciences, Matsuyama 
      University.
FAU - Yoshimura, Morio
AU  - Yoshimura M
AD  - Department of Pharmacognosy, College of Pharmaceutical Sciences, Matsuyama 
      University.
FAU - Sawamoto, Atsushi
AU  - Sawamoto A
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
FAU - Okuyama, Satoshi
AU  - Okuyama S
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
FAU - Furukawa, Yoshiko
AU  - Furukawa Y
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
FAU - Nakajima, Mitsunari
AU  - Nakajima M
AD  - Department of Pharmaceutical Pharmacology, College of Pharmaceutical Sciences, 
      Matsuyama University.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biomed Res
JT  - Biomedical research (Tokyo, Japan)
JID - 8100317
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Caloric Restriction
MH  - Drug Evaluation, Preclinical
MH  - Female
MH  - *Immunoassay/methods
MH  - Leukocytes/immunology/metabolism
MH  - Lymphocyte Activation/*immunology
MH  - Mice
MH  - RAW 264.7 Cells
MH  - Signal Transduction
MH  - T-Lymphocytes/drug effects/*immunology/*metabolism
EDAT- 2019/12/17 06:00
MHDA- 2020/04/16 06:00
CRDT- 2019/12/17 06:00
PHST- 2019/12/17 06:00 [entrez]
PHST- 2019/12/17 06:00 [pubmed]
PHST- 2020/04/16 06:00 [medline]
AID - 10.2220/biomedres.40.235 [doi]
PST - ppublish
SO  - Biomed Res. 2019;40(6):235-241. doi: 10.2220/biomedres.40.235.