PMID- 31841188
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20201109
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 23
DP  - 2019 Dec
TI  - Long noncoding RNA FAM201A mediates the metastasis of lung squamous cell cancer 
      via regulating ABCE1 expression.
PG  - 10343-10353
LID - 19672 [pii]
LID - 10.26355/eurrev_201912_19672 [doi]
AB  - OBJECTIVE: Long noncoding RNA (lncRNA) family with sequence similarity 201-member 
      A (FAM201A) is a novel lncRNA promoting the development of various cancers. 
      However, the biological function of FAM201A on the metastasis of lung squamous 
      cell carcinoma (LSCC) remains unknown. The aim of this study was to explore the 
      molecular mechanism of FAM201A and its target protein in advanced LSCC. PATIENTS 
      AND METHODS: Quantitative Polymerase Chain Reaction (qPCR) was applied to 
      evaluate FAM201A expression in lung cancer tissues. The impact of high FAM201A 
      expression on the overall survival in patients with lung cancer was tested using 
      the log-rank test. The relevance between aberrant FAM201A and clinicopathological 
      characteristics in patients with lung cancer was analyzed using the Chi-square 
      tests. Cell proliferation was assayed using the Cell Counting Kit-8 (CCK-8) and a 
      transwell assay, and the mice xenograft models were applied to determine the 
      promoting effects of FAM201A on LSCC in vitro and in vivo. The underlying 
      regulatory mechanism was explored through RNA transfection, qPCR, and Western 
      blotting. The correlation between ATP-binding cassette transporter E1 (ABCE1) and 
      FAM201A expression was verified using Spearman's correlation coefficient. 
      RESULTS: FAM201A is aberrantly elevated in tissues from patients with non-small 
      cell lung cancer. High levels of FAM201A expression were more likely to present 
      in patients with squamous type, M1 stage, and inferior overall survival. 
      Differential expression was found between non-metastatic and metastatic squamous 
      carcinoma, but not in adenocarcinoma. FAM201A knockdown inhibits cell 
      proliferation, migration, and invasion of LSCC cells in vitro, and represses 
      tumor growth in vivo. Furthermore, ABCE1 in LSCC cells was downregulated by 
      silencing FAM201A. The tissue level of ABCE1 was positively correlated with 
      FAM201A expression in patients with LSCC. CONCLUSIONS: FAM201A may markedly 
      induce migration and invasion of LSCC, resulting in the M1 stage and poor 
      survival. These findings suggest the FAM201A-ABCE1 axis as a novel therapeutic 
      target in LSCC.
FAU - He, W
AU  - He W
AD  - Department of Thoracic Surgery, General Hospital of Ningxia Medical University, 
      Yinchuan, China. heweinx@126.com.
FAU - Qiao, Z-X
AU  - Qiao ZX
FAU - Ma, B
AU  - Ma B
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (ABCE1 protein, human)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - ATP-Binding Cassette Transporters/biosynthesis/*physiology
MH  - Animals
MH  - Carcinoma, Squamous Cell/metabolism/*physiopathology
MH  - Cell Line, Tumor
MH  - Cell Movement/*physiology
MH  - Cell Proliferation/*physiology
MH  - Gene Expression Regulation, Neoplastic/physiology
MH  - Humans
MH  - Lung Neoplasms/metabolism/*physiopathology
MH  - RNA, Long Noncoding/biosynthesis/*physiology
MH  - Survival Analysis
MH  - Transfection
MH  - Xenograft Model Antitumor Assays
EDAT- 2019/12/17 06:00
MHDA- 2020/11/11 06:00
CRDT- 2019/12/17 06:00
PHST- 2019/12/17 06:00 [entrez]
PHST- 2019/12/17 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
AID - 19672 [pii]
AID - 10.26355/eurrev_201912_19672 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Dec;23(23):10343-10353. doi: 
      10.26355/eurrev_201912_19672.