PMID- 31844916
OWN - NLM
STAT- MEDLINE
DCOM- 20210611
LR  - 20210611
IS  - 1432-0827 (Electronic)
IS  - 0171-967X (Linking)
VI  - 106
IP  - 4
DP  - 2020 Apr
TI  - Neuropeptide Y1 Receptor Antagonist Alters Gut Microbiota and Alleviates the 
      Ovariectomy-Induced Osteoporosis in Rats.
PG  - 444-454
LID - 10.1007/s00223-019-00647-5 [doi]
AB  - A plethora of evidence has suggested that gut microbiota is involved in the 
      occurrence and development of postmenopausal osteoporosis (PMO). It has been 
      suggested that neuropeptide Y (NPY) modulates the bone metabolism through Y1 
      receptor (Y1R), and might be associated with gut microbiota. The present study 
      aims to evaluate the anti-osteoporotic effects of Y1R antagonist and to 
      investigate the potential mechanism by which Y1R antagonist regulates gut 
      microbiota. In this study, eighteen female rats were randomly divided into three 
      groups: the sham surgery (SHAM) group, the ovariectomized (OVX) group, and 
      OVX+BIBO3304 group. After 6 weeks following surgery, Y1R antagonist BIBO3304 was 
      administered to the rats in OVX+BIBO3304 group for 7 days. The bone 
      microstructure and serum biochemical parameters were measured at 12 weeks after 
      operation. The differences in the gut microbiota were analyzed by 16S rDNA gene 
      sequencing. Heat-map and Spearman's correlation analyses were constructed to 
      investigate the correlations between microbiota and bone metabolism-related 
      parameters. The results indicated that OVX+BIBO3304 group showed significantly 
      higher BMD, BV/TV, Tb.Th, Tb.N, Conn.D, and serum Ca(2+) level than those in OVX 
      group. Additionally, Y1R antagonist changed the gut microbiota composition with 
      lower Firmicutes/Bacteroidetes ratio and higher proportions of some probiotics, 
      including Lactobacillus. The correlation analysis showed that the changes of gut 
      microbiota were closely associated with bone microstructure and serum Ca(2+) 
      levels. Our results suggested that Y1R antagonist played an anti-osteoporotic 
      effect and regulated gut microbiota in OVX rats, indicating the potential to 
      utilize Y1R antagonist as a novel treatment for PMO.
FAU - Xie, Weixin
AU  - Xie W
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Han, Yi
AU  - Han Y
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Li, Fan
AU  - Li F
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Gu, Xiyao
AU  - Gu X
AD  - Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Su, Diansan
AU  - Su D
AD  - Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Yu, Weifeng
AU  - Yu W
AD  - Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China.
FAU - Li, Zhanchun
AU  - Li Z
AD  - Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China. 
      lzctgzyyx@163.com.
FAU - Xiao, Jie
AU  - Xiao J
AUID- ORCID: 0000-0001-5416-4754
AD  - Department of Anesthesiology, Renji Hospital, School of Medicine, Shanghai 
      Jiaotong University, 1630 Dongfang Rd, Shanghai, 200127, China. 
      applexiaomz@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191216
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (Neuropeptide Y)
RN  - 0 (Receptors, Neuropeptide Y)
SB  - IM
MH  - Animals
MH  - Bone Density/drug effects
MH  - Gastrointestinal Microbiome/*physiology
MH  - Humans
MH  - Neuropeptide Y/*metabolism
MH  - Osteoporosis/*metabolism
MH  - Osteoporosis, Postmenopausal/metabolism
MH  - Ovariectomy/*adverse effects/methods
MH  - Receptors, Neuropeptide Y/*antagonists & inhibitors
OTO - NOTNLM
OT  - Calcium absorption
OT  - Gut microbiota
OT  - Neuropeptide Y
OT  - Osteoporosis
OT  - Probiotics
EDAT- 2019/12/18 06:00
MHDA- 2021/06/12 06:00
CRDT- 2019/12/18 06:00
PHST- 2019/09/16 00:00 [received]
PHST- 2019/12/06 00:00 [accepted]
PHST- 2019/12/18 06:00 [pubmed]
PHST- 2021/06/12 06:00 [medline]
PHST- 2019/12/18 06:00 [entrez]
AID - 10.1007/s00223-019-00647-5 [pii]
AID - 10.1007/s00223-019-00647-5 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 2020 Apr;106(4):444-454. doi: 10.1007/s00223-019-00647-5. Epub 
      2019 Dec 16.