PMID- 31845571
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20200518
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 11
IP  - 51
DP  - 2019 Dec 26
TI  - Selective Regulation of Neurons, Glial Cells, and Neural Stem/Precursor Cells by 
      Poly(allylguanidine)-Coated Surfaces.
PG  - 48381-48392
LID - 10.1021/acsami.9b17143 [doi]
AB  - Poly(allylguanidine) (PAG) was synthesized and characterized as a polycationic 
      coating material for culturing neurons, glial cells, and neural stem/precursor 
      cells (NSPCs) to apply PAG for neural tissue engineering. For comparison, 
      poly-d-lysine (PDL), the golden benchmark of the neuron cell culture system, was 
      also used in this study. When PAG was subjected to a mixed culture of neurons and 
      glial cells, cell adhesion and neurite extension of neuronal cells were clearly 
      observed but only few glial cells could be found alongside the neurons. Compared 
      to PDL, the significantly lower density of the glial fibrillary acidic 
      protein-positive cells implied that PAG suppressed the glial cell development. 
      Likewise, PAG was demonstrated to dominate the differentiation of NSPCs 
      principally into neurons. To investigate whether the different effects of PAG and 
      PDL on neuron and glial cell behaviors resulted from the difference of 
      guanidinium cations and ammonium cations, poly-l-arginine (PLA) was included and 
      compared in this study. Similar to PDL, PLA supported high neuron and glial cell 
      viability simultaneously. Consequently, glial cell growth and viability 
      restrained on PAG was not only affected by the side-chain guanidino groups but 
      also by the backbone structure property. The absence of the peptide structure in 
      the backbone of PAG and the conformation of coated PAG on tissue culture 
      polystyrene possibly determined the polycationic biomaterial to limit the growth 
      of glial cells.
FAU - Ji, You-Ren
AU  - Ji YR
AUID- ORCID: 0000-0001-6394-041X
AD  - Department of Biomedical Engineering , National Taiwan University , Taipei 100 , 
      Taiwan.
FAU - Homaeigohar, Shahin
AU  - Homaeigohar S
AD  - Nanochemistry and Nanoengineering, School of Chemical Engineering, Department of 
      Chemistry and Materials Science , Aalto University , Kemistintie 1 , 00076 Aalto 
      , Finland.
FAU - Wang, Yu-Hsin
AU  - Wang YH
AD  - Department of Biomedical Engineering , National Taiwan University , Taipei 100 , 
      Taiwan.
FAU - Lin, Chen
AU  - Lin C
AD  - Department of Biomedical Engineering , National Taiwan University , Taipei 100 , 
      Taiwan.
FAU - Su, Tai-Yuan
AU  - Su TY
AD  - Department of Electrical Engineering , Yuan-Ze University , Taoyuan 320 , Taiwan.
FAU - Cheng, Ching-Chia
AU  - Cheng CC
AD  - Department of Biomedical Engineering , National Taiwan University , Taipei 100 , 
      Taiwan.
FAU - Yang, Shih-Hung
AU  - Yang SH
FAU - Young, Tai-Horng
AU  - Young TH
AUID- ORCID: 0000-0001-5338-4747
AD  - Department of Biomedical Engineering , National Taiwan University , Taipei 100 , 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20191217
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Biocompatible Materials)
RN  - 0 (Peptides)
RN  - 0 (Polymers)
RN  - 25191-17-7 (polyalanine)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Neural Stem Cells/*drug effects
MH  - Neuroglia/*drug effects
MH  - Neurons/*drug effects
MH  - Peptides/*chemistry
MH  - Polymers/*chemistry/*pharmacology
MH  - Rats
MH  - Tissue Engineering/methods
OTO - NOTNLM
OT  - glial cells
OT  - neural stem/precursor cells (NSPCs)
OT  - neurons
OT  - poly(allylguanidine) (PAG)
OT  - poly-d-lysine (PDL)
OT  - poly-l-arginine (PLA)
EDAT- 2019/12/18 06:00
MHDA- 2020/05/19 06:00
CRDT- 2019/12/18 06:00
PHST- 2019/12/18 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2019/12/18 06:00 [entrez]
AID - 10.1021/acsami.9b17143 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2019 Dec 26;11(51):48381-48392. doi: 
      10.1021/acsami.9b17143. Epub 2019 Dec 17.