PMID- 31846435
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20211204
IS  - 1479-6805 (Electronic)
IS  - 0022-0795 (Linking)
VI  - 244
IP  - 3
DP  - 2020 Mar
TI  - Selenoprotein S regulates adipogenesis through IRE1alpha-XBP1 pathway.
PG  - 431-443
LID - JOE-19-0292 [pii]
LID - 10.1530/JOE-19-0292 [doi]
AB  - The induction of endoplasmic reticulum (ER) stress is associated with 
      adipogenesis, during which the inositol-requiring enzyme 1 alpha 
      (IRE1alpha)-X-box-binding protein 1 (XBP1) pathway is involved. Selenoprotein S 
      (SelS), which is an ER resident selenoprotein, is involved in ER homeostasis 
      regulation; however, little is known about the role of SelS in regulating 
      adipogenesis. In vivo studies showed that SelS protein levels in white adipose 
      tissue were increased in obese subjects and high-fat diet (HFD)-fed mice. 
      Moreover, we identified that SelS protein levels increased in the early phase of 
      adipogenesis and then decreased in the late phase during adipogenesis. 
      Overexpression of SelS promoted adipogenesis. Conversely, knockdown (KD) of SelS 
      resulted in the inhibition of adipogenesis, which was related to increasing cell 
      death, decreased mitotic clonal expansion, and cell cycle G1 arrest. In vivo 
      studies also showed that ER stress markers (p-IRE1alpha/IRE1alpha, XBP1s, and Grp78) were 
      significantly increased with upregulating of SelS expression in subcutaneous and 
      visceral adipose tissues in the obese subjects and HFD-fed mice. Furthermore, in 
      SelS KD cells, the levels of Grp78 were increased and the levels of p-IRE1alpha/IRE1alpha 
      were unchanged , but mRNA levels of spliced XBP1 (XBP1s) produced by 
      IRE1alpha-mediated splicing were decreased, suggesting a role of SelS in the 
      modulation of IRE1alpha-XBP1 pathway. Moreover, inhibition of adipogenesis by SelS 
      suppression can be rescued by overexpression of XBP1s. Thus, SelS appears to 
      function as a novel regulator of adipogenesis through the IRE1alpha-XBP1 signaling 
      pathway.
FAU - Men, Lili
AU  - Men L
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Yao, Junjie
AU  - Yao J
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Yu, Shanshan
AU  - Yu S
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Li, Yu
AU  - Li Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Cui, Siyuan
AU  - Cui S
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Jin, Shi
AU  - Jin S
AD  - Department of Laparoscopic Surgery, The First Affiliated Hospital of Dalian 
      Medical University, Dalian, China.
FAU - Zhang, Guixin
AU  - Zhang G
AD  - Department of General Surgery, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
FAU - Ren, Decheng
AU  - Ren D
AD  - Department of Medicine, The University of Chicago, Chicago, Illinois, USA.
FAU - Du, Jianling
AU  - Du J
AD  - Department of Endocrinology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian, China.
LA  - eng
GR  - P30 DK020595/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (HSPA5 protein, human)
RN  - 0 (Hspa5 protein, mouse)
RN  - 0 (Membrane Proteins)
RN  - 0 (SELENOS protein, human)
RN  - 0 (Selenoproteins)
RN  - 0 (Selenos protein, mouse)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (XBP1 protein, human)
RN  - 0 (Xbp1 protein, mouse)
RN  - EC 2.7.11.1 (ERN1 protein, human)
RN  - EC 2.7.11.1 (Ern1 protein, mouse)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.1.- (Endoribonucleases)
SB  - IM
MH  - Adipocytes/cytology/metabolism
MH  - *Adipogenesis
MH  - Animals
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoribonucleases/genetics/metabolism
MH  - Female
MH  - Humans
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Serine-Threonine Kinases/genetics/metabolism
MH  - Selenoproteins/genetics/*metabolism
MH  - *Signal Transduction
MH  - X-Box Binding Protein 1/genetics/metabolism
OTO - NOTNLM
OT  - IRE1alpha
OT  - SelS
OT  - XBP1
OT  - adipogenesis
OT  - endoplasmic reticulum stress
EDAT- 2019/12/18 06:00
MHDA- 2020/09/01 06:00
CRDT- 2019/12/18 06:00
PHST- 2019/12/03 00:00 [received]
PHST- 2019/12/16 00:00 [accepted]
PHST- 2019/12/18 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2019/12/18 06:00 [entrez]
AID - JOE-19-0292 [pii]
AID - 10.1530/JOE-19-0292 [doi]
PST - ppublish
SO  - J Endocrinol. 2020 Mar;244(3):431-443. doi: 10.1530/JOE-19-0292.