PMID- 31846686
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 105
DP  - 2020 Apr
TI  - Functions of TNF-alpha1 and TNF-alpha2 in large yellow croaker (Larimichthys crocea) in 
      monocyte/macrophage activation.
PG  - 103576
LID - S0145-305X(19)30557-9 [pii]
LID - 10.1016/j.dci.2019.103576 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays crucial roles in cell development, 
      proliferation, apoptosis, inflammation, and immunity. TNF-alpha genes have been 
      identified in various fish species, however, their biological functions remain to 
      be further clarified. In this study, we identified a novel TNF-alpha homologue 
      (LcTNF-alpha2) from large yellow croaker (Larimichthys crocea), which shares a low 
      amino acid sequence identity to the previously reported large yellow croaker 
      TNF-alpha (LcTNF-alpha1). The open reading frame of LcTNF-alpha2 is 714 nucleotides long, 
      encoding a peptide of 237 amino acids (aa). The deduced LcTNF-alpha2 protein contains 
      a 23-aa transmembrane region, a TACE restriction site at residues T(71)/L(72), a 
      TNF family signature (I(108)- F(135)), and two conserved cysteine residues (C(39) 
      and C(179)), as found in other known TNF-alpha sequences. Both LcTNF-alpha1 and LcTNF-alpha2 
      genes were constitutively expressed in all examined tissues and significantly 
      up-regulated in the spleen and head kidney by Vibrio alginolyticus. Their 
      transcripts were also detected in primary head kidney monocytes/macrophages 
      (MO/Mvarphis), lymphocytes (PKLs), granulocytes (PKGs), and large yellow croaker head 
      kidney (LYCK) cell line and significantly increased in these cell types by 
      inactivated Vibrio alginolyticus. Recombinant LcTNF-alpha1 and LcTNF-alpha2 proteins 
      (rLcTNF-alpha1 and rLcTNF-alpha2) produced in Pichia pastoris not only significantly 
      increased the production of reactive oxygen species (ROS), but also promoted the 
      expression of proinflammatory cytokines (IL-1beta, IL-6,IL-8, and TNF-alpha1) in MO/Mvarphis 
      from large yellow croaker. Even more, after stimulation with rLcTNF-alpha1 and 
      rLcTNF-alpha2, the production of nitrogen oxide (NO) and the expression of inducible 
      NO synthase (iNOS) gene were significantly up-regulated. However, only rLcTNF-alpha1 
      remarkedly enhanced the phagocytosis of MO/Mvarphis and increased the expression of 
      TNF-alpha2 in MO/Mvarphis. These results therefore indicated that LcTNF-alpha1 and LcTNF-alpha2 
      both play roles in promoting activation of head kidney MO/Mvarphis.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Huang, Mingyue
AU  - Huang M
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Mu, Pengfei
AU  - Mu P
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; 
      College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361102, China.
FAU - Li, Xiaofeng
AU  - Li X
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Ren, Qiulei
AU  - Ren Q
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Zhang, Xiang-Yang
AU  - Zhang XY
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Mu, Yinnan
AU  - Mu Y
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Chen, Xinhua
AU  - Chen X
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; 
      Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for 
      Marine Science and Technology, Qingdao, 266071, China. Electronic address: 
      chenxinhua@tio.org.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191214
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Cytokines)
RN  - 0 (Fish Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Cytokines/metabolism
MH  - Fish Proteins/*genetics
MH  - Immunity, Innate
MH  - Macrophage Activation/genetics
MH  - Macrophages/*immunology
MH  - Monocytes/*immunology
MH  - Perciformes/*immunology
MH  - Phylogeny
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Transcriptome
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Vibrio Infections/*immunology
MH  - Vibrio alginolyticus/*physiology
OTO - NOTNLM
OT  - Inflammatory response
OT  - Large yellow croaker (Larimichthys crocea)
OT  - MO/Mvarphis activation
OT  - TNF-alpha1
OT  - TNF-alpha2
COIS- Declaration of competing interest The authors have no conflicting commercial or 
      financial interest in publishing this paper.
EDAT- 2019/12/18 06:00
MHDA- 2021/06/22 06:00
CRDT- 2019/12/18 06:00
PHST- 2019/11/09 00:00 [received]
PHST- 2019/12/13 00:00 [revised]
PHST- 2019/12/13 00:00 [accepted]
PHST- 2019/12/18 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2019/12/18 06:00 [entrez]
AID - S0145-305X(19)30557-9 [pii]
AID - 10.1016/j.dci.2019.103576 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2020 Apr;105:103576. doi: 10.1016/j.dci.2019.103576. Epub 2019 
      Dec 14.