PMID- 31846798
OWN - NLM
STAT- MEDLINE
DCOM- 20200930
LR  - 20200930
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 220
DP  - 2020 Feb
TI  - Transcriptomic profile of chicken bone marrow-derive dendritic cells in response 
      to H9N2 avian influenza A virus.
PG  - 109992
LID - S0165-2427(19)30126-6 [pii]
LID - 10.1016/j.vetimm.2019.109992 [doi]
AB  - Avian influenza subtype H9N2 infection is a mild but highly contagious disease 
      that is associated with a decrease in the efficacy of vaccine interventions, and 
      an increase in susceptibility to secondary infections in poultry. However, the 
      immune evasion mechanism of H9N2 avian influenza viruses (AIVs) in chickens is 
      poorly understood. Dendritic cells (DCs) are immune cells of major importance, 
      involved in innate immune responses against viruses, but also in the setting of 
      adaptive immune response due to their high ability to present viral antigen. 
      Therefore, in the present study we used high-throughput RNA-sequencing technology 
      at the transcriptome level to identify the differentially expressed genes (DEGs) 
      between chicken DCs infected with H9N2 virus and mock-infected DCs. We identified 
      4151 upregulated DEGs and 2138 downregulated DEGs. Further enrichment analysis 
      showed that the upregulated DEGs were enriched in the biological processes mainly 
      involved in signal transduction, transmembrane transport, and innate 
      immune/inflammatory responses. In contrast, the downregulated DEGs were 
      associated with the biological processes mainly including metabolic process, and 
      MHC class I antigen processing and presentation. In addition, 49 of these 
      immune-related DEGs were validated by reverse transcription quantitative PCR 
      (RT-qPCR). Collectively, these data suggest that H9N2 virus infection may enhance 
      the signal transduction, and innate immune responses in chicken DCs, but impair 
      their metabolic functions and antigen-presenting responses, which provide helpful 
      insight into the pathogenesis of H9N2 AIVs in chickens and managing this 
      infection in poultry farms.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Liu, Qingtao
AU  - Liu Q
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China. Electronic 
      address: taoqingliu2013@163.com.
FAU - Yang, Jing
AU  - Yang J
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Huang, Xinmei
AU  - Huang X
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Liu, Yuzhuo
AU  - Liu Y
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Han, Kaikai
AU  - Han K
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Zhao, Dongmin
AU  - Zhao D
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Zhang, Lijiao
AU  - Zhang L
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China.
FAU - Li, Yin
AU  - Li Y
AD  - Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, and 
      Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Nanjing, Jiangsu, 50 Zhongling Street, 210014, China. Electronic 
      address: muziyin08@163.com.
LA  - eng
PT  - Journal Article
DEP - 20191209
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/immunology/*virology
MH  - Cells, Cultured
MH  - Chickens
MH  - Dendritic Cells/*immunology/*virology
MH  - Down-Regulation
MH  - Gene Expression Profiling
MH  - Immune Evasion/genetics
MH  - Immunity, Innate/*genetics
MH  - Influenza A Virus, H9N2 Subtype/*immunology
MH  - Specific Pathogen-Free Organisms
MH  - Up-Regulation
OTO - NOTNLM
OT  - Antigen presentation
OT  - Dendritic cell
OT  - H9N2 avian influenza A virus
OT  - Innate immune response
OT  - RNA-sequencing
COIS- Declaration of Competing Interest None.
EDAT- 2019/12/18 06:00
MHDA- 2020/10/02 06:00
CRDT- 2019/12/18 06:00
PHST- 2019/04/08 00:00 [received]
PHST- 2019/12/05 00:00 [revised]
PHST- 2019/12/07 00:00 [accepted]
PHST- 2019/12/18 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/12/18 06:00 [entrez]
AID - S0165-2427(19)30126-6 [pii]
AID - 10.1016/j.vetimm.2019.109992 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2020 Feb;220:109992. doi: 10.1016/j.vetimm.2019.109992. 
      Epub 2019 Dec 9.