PMID- 31851880
OWN - NLM
STAT- MEDLINE
DCOM- 20200302
LR  - 20220916
IS  - 0894-0282 (Print)
IS  - 0894-0282 (Linking)
VI  - 33
IP  - 2
DP  - 2020 Feb
TI  - Tricarboxylic Acid (TCA) Cycle Enzymes and Intermediates Modulate Intracellular 
      Cyclic di-GMP Levels and the Production of Plant Cell Wall-Degrading Enzymes in 
      Soft Rot Pathogen Dickeya dadantii.
PG  - 296-307
LID - 10.1094/MPMI-07-19-0203-R [doi]
AB  - Dickeya dadantii is a plant-pathogenic bacterium that causes soft-rot in a wide 
      range of plants. Although we have previously demonstrated that cyclic 
      bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP), a bacterial 
      secondary messenger, plays a central role in virulence regulation in D. dadantii, 
      the upstream signals that modulate c-di-GMP remain enigmatic. Using a genome-wide 
      transposon mutagenesis approach of a Deltahfq mutant strain that has high c-di-GMP 
      and reduced motility, we uncovered transposon mutants that recovered the 
      c-di-GMP-mediated repression on swimming motility. A number of these mutants 
      harbored transposon insertions in genes encoding tricarboxylic acid (TCA) cycle 
      enzymes. Two of these TCA transposon mutants were studied further by generating 
      chromosomal deletions of the fumA gene (encoding fumarase) and the sdhCDAB operon 
      (encoding succinate dehydrogenase). Disruption of the TCA cycle in these deletion 
      mutants resulted in reduced intracellular c-di-GMP and enhanced production of 
      pectate lyases (Pels), a major plant cell wall-degrading enzyme (PCWDE) known to 
      be transcriptionally repressed by c-di-GMP. Consistent with this result, addition 
      of TCA cycle intermediates such as citrate also resulted in increased c-di-GMP 
      levels and decreased production of Pels. Additionally, we found that a 
      diguanylate cyclase GcpA was solely responsible for the observed citrate-mediated 
      modulation of c-di-GMP. Finally, we demonstrated that addition of citrate induced 
      not only an overproduction of GcpA protein but also a concomitant repression of 
      the c-di-GMP-degrading phosphodiesterase EGcpB which, together, resulted in an 
      increase in the intracellular concentration of c-di-GMP. In summary, our report 
      demonstrates that bacterial respiration and respiration metabolites serve as 
      signals for the regulation of c-di-GMP signaling.
FAU - Yuan, Xiaochen
AU  - Yuan X
AD  - Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu 
      Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base 
      of Ministry of Science and Technology, Nanjing, 210014, China.
AD  - Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, 
      WI 53211, U.S.A.
FAU - Zeng, Quan
AU  - Zeng Q
AD  - Department of Plant Pathology and Ecology, The Connecticut Agricultural 
      Experiment Station, New Haven, CT 06511, U.S.A.
FAU - Xu, Jingsheng
AU  - Xu J
AD  - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 
      100193, China.
FAU - Severin, Geoffrey B
AU  - Severin GB
AD  - Department of Biochemistry and Molecular Biology, Michigan State University, East 
      Lansing, MI 48824, U.S.A.
FAU - Zhou, Xiang
AU  - Zhou X
AD  - School of Forestry and Biotechnology, Zhejiang Agricultural and Forestry 
      University, Hangzhou, 311300, China.
FAU - Waters, Christopher M
AU  - Waters CM
AD  - Department of Microbiology and Molecular Genetics, Michigan State University.
FAU - Sundin, George W
AU  - Sundin GW
AD  - Department of Plant, Soil, and Microbial Sciences, Michigan State University.
FAU - Ibekwe, Abasiofiok M
AU  - Ibekwe AM
AD  - Agricultural Research Service-US Salinity Laboratory, United States Department of 
      Agriculture, Riverside, CA 92507, U.S.A.
FAU - Liu, Fengquan
AU  - Liu F
AD  - Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu 
      Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base 
      of Ministry of Science and Technology, Nanjing, 210014, China.
FAU - Yang, Ching-Hong
AU  - Yang CH
AUID- ORCID: 0000-0001-5330-7055
AD  - Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, 
      WI 53211, U.S.A.
LA  - eng
GR  - R01 GM109259/GM/NIGMS NIH HHS/United States
GR  - R03 AI130554/AI/NIAID NIH HHS/United States
GR  - R21 AI144395/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20191218
PL  - United States
TA  - Mol Plant Microbe Interact
JT  - Molecular plant-microbe interactions : MPMI
JID - 9107902
RN  - 0 (Bacterial Proteins)
RN  - 61093-23-0 (bis(3',5')-cyclic diguanylic acid)
RN  - H2D2X058MU (Cyclic GMP)
RN  - Dickeya dadantii
SB  - IM
MH  - *Bacterial Proteins/genetics/metabolism
MH  - Cell Wall/microbiology
MH  - Cyclic GMP/*analogs & derivatives/genetics/metabolism
MH  - Dickeya
MH  - *Gammaproteobacteria/enzymology/genetics
MH  - Gene Expression Regulation, Bacterial/genetics
MH  - Mutation
PMC - PMC9354473
MID - NIHMS1822306
OTO - NOTNLM
OT  - Dickeya dadantii
OT  - TCA cycle
OT  - bacterial pathogenesis
OT  - c-di-GMP
OT  - cell wall
OT  - metabolism
OT  - pectate lyase
OT  - soft rot
COIS- The author(s) declare no conflict of interest.
EDAT- 2019/12/19 06:00
MHDA- 2020/03/03 06:00
PMCR- 2022/08/05
CRDT- 2019/12/19 06:00
PHST- 2019/12/19 06:00 [pubmed]
PHST- 2020/03/03 06:00 [medline]
PHST- 2019/12/19 06:00 [entrez]
PHST- 2022/08/05 00:00 [pmc-release]
AID - 10.1094/MPMI-07-19-0203-R [doi]
PST - ppublish
SO  - Mol Plant Microbe Interact. 2020 Feb;33(2):296-307. doi: 
      10.1094/MPMI-07-19-0203-R. Epub 2019 Dec 18.