PMID- 31853306
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 19
IP  - 1
DP  - 2020 Jan
TI  - Effect of lentiviral vector-packaged interleukin-18 gene on the malignant 
      behavior of lung cancer.
PG  - 319-326
LID - 10.3892/etm.2019.8204 [doi]
AB  - Interleukin-18 (IL-18) is a multifunctional cytokine that exhibits antitumor, 
      anti-infection and immunoregulatory functions. This study aimed to investigate 
      the effects of lentiviral vector-packaged interleukin (IL)-18 gene on the 
      malignant behavior of lung cancer and the potential underlying molecular 
      mechanism of IL-18 anticancer activity. Human lung adenocarcinoma A549 cells 
      transfected with human IL-18 gene-containing lentiviral expression vector were 
      the IL-18 intervention group (group A), cells transfected with the empty 
      lentiviral expression vector were empty vector group (group B), and cells without 
      any intervention were the blank control group (group C). Reverse 
      transcription-quantitative PCR and western blotting were used to determine IL-18 
      mRNA and protein expression levels. Cell Counting Kit-8, colony-formation, flow 
      cytometry, invasion and wound-healing assays were used to evaluate the malignant 
      behavior of A549 cells transfected with the IL-18 lentiviral vector. The 
      expression levels of the T helper (Th)1 cell cytokine interferon-gamma (IFN-gamma) and 
      the Th2 cell cytokine IL-4 were tested by ELISA, and western blotting was used to 
      test the expressing of nuclear factor kappaB (NF-kappaB). The results demonstrated that 
      IL-18 mRNA and protein expression levels in group A were significantly increased 
      compared with groups B and C; the expression levels of IFN-gamma in group A were 
      higher and the expression levels of IL-4 in group A were lower compared with 
      those in groups B and C; and the expression of NF-kappaB was increased in the 
      cytoplasm and decreased in the nucleus in group A compared with groups B and C. 
      The data indicated that, compared with the control groups, the IL-18 gene 
      lentiviral expression vector increased the expression of IL-18, diminished A549 
      cell proliferative ability, enhanced apoptosis, decreased the invasive and 
      metastatic capacities of the cells, promoted the secretion of IFN-gamma, decreased 
      the production of IL-4, reversed the imbalance of Th1/Th2 cell subsets and 
      inhibited the nuclear activation of NF-kappaB, which collectively present an 
      anti-lung cancer mechanism and deserve further study.
CI  - Copyright (c) 2019, Spandidos Publications.
FAU - Chen, Xiangqi
AU  - Chen X
AD  - Teaching and Research Department of Respiratory Medicine, Union Clinical Medical 
      College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
AD  - Department of Respiratory Medicine, Fujian Medical University Union Hospital, 
      Fuzhou, Fujian 350001, P.R. China.
FAU - Feng, Rui
AU  - Feng R
AD  - Department of Oncology Medicine, Fujian Medical University Union Hospital, 
      Fuzhou, Fujian 350001, P.R. China.
FAU - Xiong, Donglan
AU  - Xiong D
AD  - Teaching and Research Department of Respiratory Medicine, Union Clinical Medical 
      College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
FAU - Yang, Sheng
AU  - Yang S
AD  - Department of Oncology Medicine, Fujian Medical University Union Hospital, 
      Fuzhou, Fujian 350001, P.R. China.
AD  - Teaching and Research Department of Oncology Medicine, Union Clinical Medical 
      College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
AD  - Fujian Key Laboratory of Translational Cancer Medicine, Fuzhou, Fujian 350001, 
      P.R. China.
AD  - Fujian Medical University Stem Cell Research Institute, Fuzhou, Fujian 350001, 
      P.R. China.
FAU - Lin, Tingyan
AU  - Lin T
AD  - Teaching and Research Department of Respiratory Medicine, Union Clinical Medical 
      College of Fujian Medical University, Fuzhou, Fujian 350001, P.R. China.
AD  - Department of Respiratory Medicine, Fujian Medical University Union Hospital, 
      Fuzhou, Fujian 350001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20191115
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6909666
OTO - NOTNLM
OT  - biological behavior
OT  - interleukin-18 gene
OT  - lentivirus
OT  - lung cancer
OT  - molecular mechanism
EDAT- 2019/12/20 06:00
MHDA- 2019/12/20 06:01
PMCR- 2019/11/15
CRDT- 2019/12/20 06:00
PHST- 2018/07/13 00:00 [received]
PHST- 2019/06/27 00:00 [accepted]
PHST- 2019/12/20 06:00 [entrez]
PHST- 2019/12/20 06:00 [pubmed]
PHST- 2019/12/20 06:01 [medline]
PHST- 2019/11/15 00:00 [pmc-release]
AID - ETM-0-0-8204 [pii]
AID - 10.3892/etm.2019.8204 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Jan;19(1):319-326. doi: 10.3892/etm.2019.8204. Epub 2019 Nov 
      15.