PMID- 31853379
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1976-8354 (Print)
IS  - 2151-2485 (Electronic)
IS  - 1976-8354 (Linking)
VI  - 23
IP  - 6
DP  - 2019
TI  - Shwachman-Bodian-Diamond syndrome protein desensitizes breast cancer cells to 
      apoptosis in stiff matrices by repressing the caspase 8-mediated pathway.
PG  - 414-421
LID - 10.1080/19768354.2019.1666030 [doi]
AB  - Certain cancer types, including breast cancer, are accompanied with stiffening of 
      the surrounding extracellular matrix (ECM). Previous studies suggest that this 
      stiffened matrix influences cancer cell progression, such as proliferation and 
      invasion, both biochemically and mechanically. However, the contribution of ECM 
      stiffness to cellular response to diverse stresses, which most cancer cells are 
      exposed to, has not been elucidated. In this study, we demonstrate that 
      expression of the Shwachman-Bodian-Diamond syndrome protein (SDBS) in a stiff 
      matrix protects cells from apoptosis induced by environmental stress, including 
      anticancer drugs. Cells cultured on stiff matrices were less apoptotic process 
      induced by serum depletion than those cultured on the soft matrix. Interestingly, 
      knockdown (KD) of SDBS among the apoptosis-related genes significantly increased 
      apoptosis induced by serum depletion in cells cultured in a stiff matrix. 
      Apoptosis of SDBS KD cells in a stiff matrix was significantly inhibited by the 
      caspase 8 inhibitor, indicating that activation of the caspase 8 pathway by SDBS 
      KD is critical for cancer cell apoptosis in stiff matrices. Additionally, we also 
      found that downregulation of SDBS also effectively increased cell death induced 
      by anticancer drugs, including paclitaxel, cisplatin, and eribulin. Taken 
      together, our findings suggest that inhibition of SDBS enhances effective 
      chemotherapy of malignant breast cancer cells in stiff ECM environments.
CI  - (c) 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & 
      Francis Group.
FAU - Lee, Jieun
AU  - Lee J
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Ko, Panseon
AU  - Ko P
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - You, Eunae
AU  - You E
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Jeong, Jangho
AU  - Jeong J
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Keum, Seula
AU  - Keum S
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Kim, Jaegu
AU  - Kim J
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Rahman, Mizanur
AU  - Rahman M
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Lee, Dong Ho
AU  - Lee DH
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
FAU - Rhee, Sangmyung
AU  - Rhee S
AD  - Department of Life Science, College of Natural Sciences, Chung-Ang University, 
      Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20190920
PL  - England
TA  - Anim Cells Syst (Seoul)
JT  - Animal cells and systems
JID - 101478641
PMC - PMC6913620
OTO - NOTNLM
OT  - ECM stiffness
OT  - SBDS
OT  - apoptosis
OT  - breast cancer
OT  - caspase
EDAT- 2019/12/20 06:00
MHDA- 2019/12/20 06:01
PMCR- 2019/09/20
CRDT- 2019/12/20 06:00
PHST- 2019/08/07 00:00 [received]
PHST- 2019/08/25 00:00 [revised]
PHST- 2019/08/26 00:00 [accepted]
PHST- 2019/12/20 06:00 [entrez]
PHST- 2019/12/20 06:00 [pubmed]
PHST- 2019/12/20 06:01 [medline]
PHST- 2019/09/20 00:00 [pmc-release]
AID - 1666030 [pii]
AID - 10.1080/19768354.2019.1666030 [doi]
PST - epublish
SO  - Anim Cells Syst (Seoul). 2019 Sep 20;23(6):414-421. doi: 
      10.1080/19768354.2019.1666030. eCollection 2019.