PMID- 31853722
OWN - NLM
STAT- MEDLINE
DCOM- 20200504
LR  - 20220411
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 251
IP  - 1
DP  - 2019 Dec 18
TI  - Regulation of color transition in purple tea (Camellia sinensis).
PG  - 35
LID - 10.1007/s00425-019-03328-7 [doi]
AB  - Comparative proteomics and metabolomics study of juvenile green, light purple and 
      dark purple leaf to identify key proteins and metabolites that putatively govern 
      color transition in Camellia sinensis. Color transition from juvenile green to 
      dark purple leaf in Camellia sinensis is a complex process and thought to be 
      regulated by an intricate balance of genes, proteins and metabolites expression. 
      A molecular-level understanding of proteins and metabolites expression is needed 
      to define metabolic process underpinning color transition in C. sinensis. Here, 
      purple leaf growth of C. sinensis cultivar was divided into three developmental 
      stages viz. juvenile green (JG), light purple (LP) and dark purple (DP) leaf. 
      Scanning electron microscope (SEM) analysis revealed a clear morphological 
      variation such as cell size, shape and texture as tea leaf undergoing color 
      transition. Proteomic and metabolomic analyses displayed the temporal changes in 
      proteins and metabolites that occur in color transition process. In total, 211 
      differentially expressed proteins (DEPs) were identified presumably involved in 
      secondary metabolic processes particularly, flavonoids/anthocyanin biosynthesis, 
      phytohormone regulation, carbon and nitrogen assimilation and photosynthesis, 
      among others. Subcellular localization of three candidate proteins was further 
      evaluated by their transient expression in planta. Interactome study revealed 
      that proteins involved in primary metabolism, precursor metabolite, 
      photosynthesis, phytohormones, transcription factor and anthocyanin biosynthesis 
      were found to be interact directly or indirectly and thus, regulate color 
      transition from JG to DP leaf. The present study not only corroborated earlier 
      findings but also identified novel proteins and metabolites that putatively 
      govern color transition in C. sinensis. These findings provide a platform for 
      future studies that may be utilized for metabolic engineering/molecular breeding 
      in an effort to develop more desirable traits.
FAU - Kumari, Manglesh
AU  - Kumari M
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
AD  - Academy of Scientific and Innovative Research, New Delhi, India.
FAU - Thakur, Shweta
AU  - Thakur S
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
AD  - Academy of Scientific and Innovative Research, New Delhi, India.
FAU - Kumar, Ajay
AU  - Kumar A
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
FAU - Joshi, Robin
AU  - Joshi R
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
FAU - Kumar, Prakash
AU  - Kumar P
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
AD  - Academy of Scientific and Innovative Research, New Delhi, India.
FAU - Shankar, Ravi
AU  - Shankar R
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India.
FAU - Kumar, Rajiv
AU  - Kumar R
AUID- ORCID: 0000-0002-2559-674X
AD  - Department of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology, 
      Palampur, 176061, HP, India. rajiv@ihbt.res.in.
LA  - eng
GR  - MLP-0201/Council of Scientific and Industrial Research, India/
PT  - Journal Article
DEP - 20191218
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 0 (Anthocyanins)
RN  - 0 (Flavonoids)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Plant Proteins)
RN  - 0 (Tea)
RN  - 0 (Transcription Factors)
RN  - 1406-65-1 (Chlorophyll)
RN  - 7440-44-0 (Carbon)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Anthocyanins/biosynthesis
MH  - Camellia sinensis/genetics/*metabolism/*radiation effects
MH  - Carbon/metabolism
MH  - Cell Size
MH  - Chlorophyll/analysis
MH  - Color
MH  - Flavonoids/biosynthesis
MH  - Gene Expression Regulation, Plant
MH  - *Light
MH  - Metabolic Networks and Pathways
MH  - Metabolomics
MH  - Nitrogen/metabolism
MH  - Photosynthesis
MH  - Plant Growth Regulators
MH  - Plant Leaves/cytology/growth & development/*metabolism/*radiation effects
MH  - Plant Proteins/genetics/metabolism
MH  - Protein Interaction Maps
MH  - Proteomics
MH  - Secondary Metabolism
MH  - Tea
MH  - Transcription Factors
MH  - Transcriptome
OTO - NOTNLM
OT  - Leaf
OT  - Metabolomics
OT  - Proteomics
OT  - Secondary metabolism
EDAT- 2019/12/20 06:00
MHDA- 2020/05/06 06:00
CRDT- 2019/12/20 06:00
PHST- 2019/08/22 00:00 [received]
PHST- 2019/12/09 00:00 [accepted]
PHST- 2019/12/20 06:00 [entrez]
PHST- 2019/12/20 06:00 [pubmed]
PHST- 2020/05/06 06:00 [medline]
AID - 10.1007/s00425-019-03328-7 [pii]
AID - 10.1007/s00425-019-03328-7 [doi]
PST - epublish
SO  - Planta. 2019 Dec 18;251(1):35. doi: 10.1007/s00425-019-03328-7.