PMID- 31854883 OWN - NLM STAT- MEDLINE DCOM- 20200106 LR - 20200108 IS - 0250-3301 (Print) IS - 0250-3301 (Linking) VI - 40 IP - 9 DP - 2019 Sep 8 TI - [Effect of Signal Molecule Combined with Thiobacillus denitrificans on Simultaneous Removal of Nitrogen and Sulfur]. PG - 4177-4184 LID - 10.13227/j.hjkx.201903012 [doi] AB - The effects of Thiobacillus denitrificans combined with signal molecules on the removal of sulfide and nitrate was investigated. By adding signal molecules and T. denitrificans at the same, the total number of microorganisms increased, the removal of sulfide and nitrate was accelerated, and an increase in nitrogen gas and more stable accumulation of elemental sulfur was observed. The total number of microorganisms after the reaction was detected using fluorescence in situ hybridization (FISH) technique. In this experiment, the optimal concentration for the stable accumulation of elemental sulfur from six concentrations of signal molecules was revealed. Further, the effects of adding signal molecules, T. denitrificans, and their combination were analyzed at this concentration. The results showed that it was easier to accumulate elemental sulfur after the addition of 1.0 mumol.L(-1) signal molecule. After adding both T. denitrificans and 1.0 mumol.L(-1) signal molecules at a sulfide concentration of 200 mg.L(-1), the removal of sulfide and nitrate increased to 99.8% and 96.9% at 72 h, respectively, and increases in nitrogen gas and sulfur were observed. The amounts of elemental sulfur and nitrogen gas reached to 59.0 mg and 80.0 mL, respectively, after adding 2.5 mumol.L(-1) signal molecules at 72 h when the sulfide concentration was 300 mg.L(-1). Under those conditions, the removal efficiency of sulfide and nitrate reached 99.0% and 93.9%, and the production of elemental sulfur and nitrogen reached 63.1 mg and 79.5 mL, respectively. FAU - Xu, Jin-Lan AU - Xu JL AD - School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. AD - Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China. FAU - Chen, Zi-Wei AU - Chen ZW AD - School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. AD - Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China. FAU - Zhang, Shao-Feng AU - Zhang SF AD - School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. AD - Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China. FAU - Wei, Yi-Ni AU - Wei YN AD - School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China. AD - Shaanxi Key Laboratory of Environmental Engineering, Xi'an 710055, China. LA - chi PT - Journal Article PL - China TA - Huan Jing Ke Xue JT - Huan jing ke xue= Huanjing kexue JID - 8405344 RN - 0 (Nitrates) RN - 0 (Sulfides) RN - 70FD1KFU70 (Sulfur) RN - N762921K75 (Nitrogen) SB - IM MH - Bioreactors MH - In Situ Hybridization, Fluorescence MH - Nitrates MH - *Nitrogen/metabolism MH - Sulfides MH - *Sulfur/metabolism MH - *Thiobacillus OTO - NOTNLM OT - Thiobacillus denitrificans OT - elemental sulfur OT - fluorescence in situ hybridization (FISH) OT - signal molecule OT - simultaneous removal of nitrogen and sulfur EDAT- 2019/12/20 06:00 MHDA- 2020/01/07 06:00 CRDT- 2019/12/20 06:00 PHST- 2019/12/20 06:00 [entrez] PHST- 2019/12/20 06:00 [pubmed] PHST- 2020/01/07 06:00 [medline] AID - 10.13227/j.hjkx.201903012 [doi] PST - ppublish SO - Huan Jing Ke Xue. 2019 Sep 8;40(9):4177-4184. doi: 10.13227/j.hjkx.201903012.